A5034762378- Advanced biosensing and bioanalysis techniques
- Advanced Fluorescence Microscopy Techniques
- Protein Degradation and Inhibitors
- RNA Interference and Gene Delivery
- Multiple Myeloma Research and Treatments
- Analytical Chemistry and Chromatography
- Histone Deacetylase Inhibitors Research
- Advanced Drug Delivery Systems
- Advanced Biosensing Techniques and Applications
- Nanoplatforms for cancer theranostics
- Conducting polymers and applications
- Wireless Sensor Networks and IoT
- Construction Engineering and Safety
- Immunotherapy and Immune Responses
- Analytical Methods in Pharmaceuticals
- Dendrimers and Hyperbranched Polymers
- Geotechnical Engineering and Analysis
- Ubiquitin and proteasome pathways
- Biosensors and Analytical Detection
- Nanoparticle-Based Drug Delivery
- Antibiotic Resistance in Bacteria
- Advanced Electron Microscopy Techniques and Applications
- Electrochemical sensors and biosensors
- Gaze Tracking and Assistive Technology
- Advancements in Photolithography Techniques
The Bristol-Myers Squibb Children's Hospital
2024-2025
Henan University of Traditional Chinese Medicine
2021-2024
First Affiliated Hospital of Henan University
2021-2024
Bristol-Myers Squibb (Germany)
2024
Jinan University
2024
Bristol-Myers Squibb (United States)
2021-2022
Guangdong Province Women and Children Hospital
2018-2022
Guangzhou Medical University
2022
Sun Yat-sen University
2003-2021
Analysis Group (United States)
2014-2020
Synthetic nanoparticles coated with cellular membranes have been increasingly explored to harness natural cell functions toward the development of novel therapeutic strategies. Herein, we report on a unique bacterial membrane-coated nanoparticle system as new and exciting antibacterial vaccine. Using Escherichia coli model pathogen, collect outer membrane vesicles (OMVs) successfully coat them onto small gold (AuNPs) diameter 30 nm. The resulting AuNPs (BM-AuNPs) show markedly enhanced...
Adsorbing small charged nanoparticles onto the outer surfaces of liposomes has become an effective strategy to stabilize against fusion prior "seeing" target bacteria, yet allow them fuse with bacteria upon arrival at infection sites. As a result, nanoparticle-stabilized have emerging drug delivery platform for treatment various bacterial infections. To facilitate translation this clinical tests and uses, herein we integrate hydrogel technology more sustained topical delivery. The...
Adsorbing small charged nanoparticles onto liposome surfaces to stabilize them against fusion and payload leakage has resulted in a new class of liposomes capable environment-responsive drug delivery. Herein, we engineered formulation with lipid composition sensitive bacterium-secreted phospholipase A2 (PLA2) adsorbed chitosan-modified gold (AuChi) the surface. The resulting AuChi-stabilized (AuChi-liposomes) showed prohibited activity negligible leakage. However, upon exposure either...
Abstract Progesterone is often used to protect the endometrium and prevent endometrial cancer. An intensive study on its molecular mechanism in cancer would contribute development of more promising therapies. Relevant lncRNAs mRNAs expression data cell line Ishikawa pretreated post‐treated with progesterone were derived from Gene Expression Omnibus (accession no. GSE29435), then we analyzed long noncoding RNAs differential expressions two different conditions. The Cytoscape software,...
Abstract Traces from single-molecule fluorescence microscopy (SMFM) experiments exhibit photophysical artifacts that typically necessitate human expert screening, which is time-consuming and introduces potential for user-dependent expectation bias. Here, we use deep learning to develop a rapid, automatic SMFM trace selector, termed AutoSiM, improves the sensitivity specificity of an assay DNA point mutation based on recognition through equilibrium Poisson sampling (SiMREPS). The improved...
Single-molecule fluorescence resonance energy transfer (smFRET) has emerged as a pivotal technique for probing biomolecular dynamics over time at nanometer scales. Quantitative analyses of smFRET traces remain challenging due to confounding factors such low signal-to-noise ratios, photophysical effects bleaching and blinking, the complexity modeling underlying states kinetics. The dynamic distance information shaping trace powerfully uncovers even transient conformational changes in single...
Surface-enhanced Raman spectroscopy (SERS) benefits from the enhanced electromagnetic field of localized surface plasmon resonance effect metallic (especially coinage metals) nanoparticles or nanostructures. The detection sensitivity and reproducibility SERS measurement appear to be two critical issues in SERS. To solve problem associated with traditional nanoparticle aggregates substrates, we propose this work single particle We prepared uniform gold microspheres controllable size roughness...
Covalently cross-linked, three-dimensional nanodomained networks were prepared from radially layered poly(amidoamine−organosilicon) (PAMAMOS) copolymeric dendrimers containing hydrophilic polyamidoamine (PAMAM) interiors and reactive hydrophobic organosilicon (OS) exteriors. Two different types of curing chemistries used, including (a) a sol−gel-type hydrolysis alkoxysilyl-terminated followed by silanol condensation reaction (b) irradiation or heat-induced, free-radical double-bond opening...
Abstract Purpose Stemming from a myriad of genetic and epigenetic alterations, triple-negative breast cancer (TNBC) is tied to poor clinical outcomes aspires for individualized therapies. Here we investigated the therapeutic potential co-inhibiting integrin-dependent signaling pathway BRD4, transcriptional mediator, TNBC. Methods Two independent patient cohorts were subjected bioinformatic IHC examination association candidate drivers. The efficacy biological bases co-targeting these drivers...
Subcellular organelle-specific reagents for simultaneous tumor targeting, imaging, and treatment are of enormous interest in cancer therapy. Herein, we present a mitochondria targeting micelle (PEG-AIE-TPP) by conjugating triphenylphosphonium (TPP) with fluorogen which can undergo aggregation-induced emission (AIE). At first, the vitro vivo properties PEG-AIE-TPP were characterized detail. It was found that reasonably stable at physiological pH highly sensitive to mildly acidic stimuli....
Abstract Gram staining can classify bacterial species into two large groups based on cell wall differences. Our study revealed that within the same gram group (gram‐positive or gram‐negative), subtle variations alter outcomes, with peptidoglycan layer and lipid content significantly influencing this effect. Thus, bacteria also be differentiated by their spectra. Using hyperspectral microscopy, we identified six of intestinal 98.1% accuracy. demonstrated selecting right spectral band...
The translational and rotational dynamics of anisotropic optical nanoprobes revealed in single particle tracking (SPT) experiments offer molecular-level information about cellular activities. Here, we report an automated high-speed multidimensional SPT system integrated with a deep learning algorithm for the 3D orientation gold nanoparticle probes living cells high localization precision (<10 nm) temporal resolution (0.9 ms), overcoming limitations under low signal-to-noise ratio (S/N)...
Modern data-intensive techniques offer ever deeper insights into biology, but render the process of discovery increasingly complex. For example, exploiting unique ability single-molecule fluorescence microscopy (SMFM)
This study presents a new ac circuit element – the mutual capacitor, being dual of inductor, which is also transformer. characteristic mutual-capacitance coupling multi-capacitance system. A unity-coupled capacitor works as an ideal current or voltage transformer, and incidentally acts waveform separating when inductor employed converting from square-wave to quasi-sine filtering, between ports. As easy design, for heat cooling, more accurate transformation, dissipating less energy well...
Shield tunneling in karst areas poses significant challenges, as vibration caused by the shield machine can disturb stability of caves, ultimately resulting collapse a tunnel. In present study, numerical model involving an iteration process was developed based on Mindlin solution scheme to identify optimal speed for minimizing disturbance cave stability. The then implemented investigate underground tunnel constructed region with different strategies. By using variation energy density...
A new glucometer-based enzyme immunoassay was developed for the sensitive detection of alpha-fetoprotein using biofunctional dendritic polyaniline nanofibers as signal-transduction tag.
The relationships between polymer molecular weight, surface roughness measured by Atomic Force Microscopy (AFM), and EUV line edge (LER), were studied in four separate rounds of experiments. In Round 1, EUV-2D (XP98248B) was prepared with seven levels added base. These resists patterned using lithography; the LER determined 100 nm dense lines. resist dramatically decreases increasing level results compared these after development for unexposed DUV (248 nm) exposed surfaces. Rounds 2-4, we...