A5002749522- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Reproductive Biology and Fertility
- 3D Printing in Biomedical Research
- Mesenchymal stem cell research
- Neurogenesis and neuroplasticity mechanisms
- Genetic Neurodegenerative Diseases
- Epigenetics and DNA Methylation
- Neurological disorders and treatments
- Mitochondrial Function and Pathology
- High Entropy Alloys Studies
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
- Cardiomyopathy and Myosin Studies
- Sperm and Testicular Function
- Educational Research and Pedagogy
- Additive Manufacturing and 3D Printing Technologies
- Demographic Trends and Gender Preferences
- Education and Learning Interventions
- Hematopoietic Stem Cell Transplantation
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Biomedical Ethics and Regulation
- Educational Systems and Policies
- MicroRNA in disease regulation
Harvard University
2024
Massachusetts General Hospital
2024
Konkuk University
2018-2024
Jeju National University
2017
ABSTRACT Huntington’s disease (HD), due to expansion of a CAG repeat in HTT , is representative growing number disorders involving somatically unstable short tandem repeats. We find that overlapping and distinct genetic modifiers clinical landmarks somatic blood DNA reveal an underlying complexity cell-type specificity the mismatch repair-related processes influence timing. Differential capture non-DNA-repair gene by multiple measures cognitive motor dysfunction argues additionally for...
Many Mendelian disorders, such as Huntington’s disease (HD) and spinocerebellar ataxias, arise from expansions of CAG trinucleotide repeats. Despite the clear genetic causes, additional factors may influence rate those monogenic disorders. Notably, genome-wide association studies discovered somewhat expected modifiers, particularly mismatch repair genes involved in repeat instability, impacting age at onset HD. Strikingly, FAN1 , previously unrelated to produced strongest HD modification...
Spermatogonial stem cells (SSCs) derived from mouse testis are unipotent in regard of spermatogenesis. Our previous study demonstrated that SSCs can be fully reprogrammed into pluripotent cells, so called germline-derived (gPS cells), on feeder (mouse embryonic fibroblasts), which supports SSC proliferation and induction pluripotency. Because an uncontrollable microenvironment caused by interactions with feeder-based reprogramming is not suitable for elucidation the self-reprogramming...
In induced pluripotent stem cells (iPSCs), pluripotency is artificially by introducing the transcription factors Oct4, Sox2, Klf4, and c-Myc.When a transgene introduced using viral vector, may be integrated into host genome cause mutation cancer.No integration occurs when an episomal vector used, but this method has limitation in that remnants of virus or remain cell, which limits use such iPSCs therapeutic applications.Chemical reprogramming, relies on treatment with small-molecule...
Oligodendrocyte progenitor cells (OPCs) are attracting attention as the ideal cell therapy for spinal cord injury (SCI). Recently, advanced programming techniques have made it possible to generate patient-specific treating SCI. In present study, we used directly-induced neural stem from fibroblasts establish OPCs (DN-OPCs) capable of proliferation, and confirmed their OPC-specific characteristics. Also, evaluated effect transplanted DN-OPCs on SCI in rats. The exhibited an phenotype...
Spermatogonial stem cells (SSCs) are unipotent adult cells, capable of differentiating into sperm cells. SSCs can be cultured in vitro for a long time. expressing Oct4, pluripotency marker, and the only which induced under defined culture conditions. However, because 2D imposes limitations cell junction formation, shape, metabolism, response to stimuli, interface with medium, mechanistic studies on reprogramming using feeder still have many challenges. Recent shown that system bio-matrix...
Umbilical cord blood (UCB) is an alternative source of allogeneic hematopoietic stem cells (HSCs) for transplantation to treat various hematological disorders. The major limitation the use UCB-derived HSCs (UCB-HSCs) in transplantation, however, low numbers a unit blood. To overcome this limitation, cytokines or small molecules have been used expand UCB-HSCs ex vivo. In study, we investigated synergistic effect combination HIL-6, SR1, and UM171 on UCB-HSC culture found that resulted highest...
Stem cells are an important therapeutic source for recovery and regeneration, as their ability of self-renewal differentiation offers unlimited supply highly specialized transplantation. Growth factors serum essential maintaining the characteristics stem in culture inducing differentiation. Because growth produced mainly bacterial (Escherichia coli) or animal cells, use such raises safety concerns that need to be resolved commercialization cell therapeutics. To overcome this problem, studies...
Recently, efforts have been made to enhance educational competency based on Computational Thinking (CT) in relation informatics education at home and abroad. Although many teachers training programs are steadily operated order cultivate the capacity response changing demands of times, this is done without acceptance an opinion process teachers’ needs. Therefore, study analyzed recognition needs model developed for secondary school. Educational were using Borich’s formula. The results as...
Genomic imprinting modulates growth and development in mammals is associated with genetic disorders. Although uniparental embryonic stem cells have been used to study genomic imprinting, there an ethical issue the destruction of human embryos. In this study, investigate status neurodevelopment, we induced pluripotent (iPSCs) that possessed only maternal alleles differentiated into neural cell lineages.Human somatic iPSCs (hSiPSCs) parthenogenetic (hPgiPSCs) were (NSCs) named hSi-NSCs...
Neural stem cells (NSCs) are defined by their ability to self-renew and generate various cell types within the nervous system. Understanding underlying mechanism which NSCs proliferate differentiate is crucial for efficient modulation of in vivo neurogenesis. MicroRNAs small non-coding RNAs controlling gene expression concerned post-transcriptional control blocking messenger RNA (mRNA) translation or degrading mRNA. play a role as modulators matching target mRNAs. Recent studies have...
Myofibrillar myopathy 6 (MFM6) is a rare childhood-onset characterized by myofibrillar disintegration, muscle weakness, and cardiomyopathy. The genetic cause of MFM6 p.Pro209Leu mutation (rs121918312-T) in the
A human induced pluripotent stem cell (iPSC) line (KKUi002-A) was generated from a skin fibroblast of 57-years-old (at sampling) male patient diagnosed with sporadic Parkinson's disease (PD). non-integration system used to reprogram fibroblasts into iPSCs by an episomal vector (OCT4/p53, SOX2/KLF4, L-MYC/LIN28). The KKUi002-A displayed typical iPSC morphology, expressed pluripotency markers, differentiated derivatives three germ layers, and had normal karyotype. These PD-derived can be...
Spermatogonial stem cells (SSCs) are the most primitive in spermatogenesis and only adult capable of passing on genome a given species to next generation. SSCs known exhibit high Oct4 expression can be induced self-reprogram into pluripotent depending culture conditions. Epigenetic modulation is well involved induction pluripotency somatic cells. However, epigenetic self-reprogramming has not been studied.In this study, we examined involvement by assessing whether enhanced treatment with...
OCT4 and NANOG are core transcription factor genes in self-renewal, differentiation, reprogramming. Here, we generated an OCT4-EGFP, NANOG-tdTomato dual reporter hiPSC line, KKUi001-A, on the basis of human induced pluripotent stem cells using CRISPR/Cas9 technology. EGFP tdTomato were inserted into before stop codon NANOG, respectively. Simultaneous expression was observed when changed during differentiation KKUi001-A line will be a useful tool to find initial time point reprogramming...