A5103203567- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Tissue Engineering and Regenerative Medicine
- Mesenchymal stem cell research
- Genomics and Chromatin Dynamics
- Muscle Physiology and Disorders
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- DNA Repair Mechanisms
- Peptidase Inhibition and Analysis
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- RNA regulation and disease
- Genetics, Aging, and Longevity in Model Organisms
Max Planck Institute for Molecular Biomedicine
2009-2025
University of Münster
2021
During the first lineage segregation, mammalian embryos generate inner cell mass (ICM) and trophectoderm (TE). ICM gives rise to epiblast (EPI) that forms all types of body, an ability referred as pluripotency. The molecular mechanisms induce pluripotency in remain incompletely elucidated. Using knockout (KO) mouse models conjunction with low-input ATAC-seq RNA-seq, we found Oct4 Sox2 gradually come into play early ICM, coinciding initiation expression. activate pluripotency-related genes...
An engineered SOX17 variant with point mutations within its DNA binding domain termed SOX17FNV is a more potent pluripotency inducer than SOX2, yet the underlying mechanism remains unclear. Although wild-type was incapable of inducing pluripotency, outperformed SOX2 in mouse and human reprogramming. In embryonic stem cells, could replace to maintain despite considerable sequence differences upregulated genes expressed cleavage-stage embryos. Mechanistically, co-bound OCT4 cooperatively...
Abstract tRNA modifications tune translation rates and codon optimality, thereby optimizing co-translational protein folding, but how optimality defects trigger cellular phenotypes remains unclear. Here, we show that ribosomes stall at specific modification-dependent pairs, triggering ribosome collisions inducing a coordinated hierarchical response of quality control pathways. Ribosome profiling reveals an unexpected functional diversity for wobble-uridine (U 34 ) during decoding. The same...
In induced pluripotent stem cells (iPSCs), pluripotency is artificially by introducing the transcription factors Oct4, Sox2, Klf4, and c-Myc.When a transgene introduced using viral vector, may be integrated into host genome cause mutation cancer.No integration occurs when an episomal vector used, but this method has limitation in that remnants of virus or remain cell, which limits use such iPSCs therapeutic applications.Chemical reprogramming, relies on treatment with small-molecule...
Article22 June 2021Open Access Source DataTransparent process Residual pluripotency is required for inductive germ cell segregation Shinya Aramaki Department of Cell and Developmental Biology, Max Planck Institute Molecular Biomedicine, Münster, Germany Search more papers by this author Saya Kagiwada Guangming Wu Guangzhou Regenerative Medicine Health Guangdong Laboratory, Guangzhou, China David Obridge Kenjiro Adachi Eva Kutejova Heiko Lickert Helmholtz Zentrum München, Deutsches...
In vitro culture systems that structurally model human myogenesis and promote PAX7 + myogenic progenitor maturation have not been established. Here we report skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm neuromesodermal progenitors develop into organized structures reassembling neural plate border dermomyotome. Culture conditions instigate lineage arrest fetal hypaxial towards limb axial anatomical identity, with...
Abstract Pioneer transcription factors are proteins that induce cellular identity transitions by binding to inaccessible regions of DNA in nuclear chromatin. They contribute chromatin opening and recruit other regulatory elements. The structural features dynamics modulating their interaction with nucleosomes still unresolved. From a combination experiments molecular simulations, we reveal here how the pioneer factor master regulator pluripotency, Oct4, interprets enhances nucleosome...
In vitro culture systems that structurally model human myogenesis and promote PAX7 + myogenic progenitor maturation have not been established. Here we report skeletal muscle organoids can be differentiated from induced pluripotent stem cell lines to contain paraxial mesoderm neuromesodermal progenitors develop into organized structures reassembling neural plate border dermomyotome. Culture conditions instigate lineage arrest fetal hypaxial towards limb axial anatomical identity, with...