A5064838965- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Antibiotic Use and Resistance
- Antifungal resistance and susceptibility
- Antimicrobial Resistance in Staphylococcus
- Antibiotics Pharmacokinetics and Efficacy
- Pneumocystis jirovecii pneumonia detection and treatment
- Mycobacterium research and diagnosis
- Respiratory viral infections research
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Enterobacteriaceae and Cronobacter Research
- Cystic Fibrosis Research Advances
- Infective Endocarditis Diagnosis and Management
- Diphtheria, Corynebacterium, and Tetanus
- Fungal Infections and Studies
- Urinary Tract Infections Management
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
- HIV/AIDS Research and Interventions
- Biosensors and Analytical Detection
- Pneumonia and Respiratory Infections
- Viral gastroenteritis research and epidemiology
- Advanced Chemical Sensor Technologies
- Plant Pathogenic Bacteria Studies
University of North Carolina at Chapel Hill
2014-2025
University of North Carolina Health Care
2012-2024
University of Pennsylvania
2014-2020
Community Link
2020
University of North Carolina Hospitals
2020
Children's Hospital of Philadelphia
2020
Engineering Associates (United States)
2017-2018
Hospital of the University of Pennsylvania
2015-2018
Tel Aviv Sourasky Medical Center
2017
Brown University
2008-2011
Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and alpha strains. The product of is tetraploid a/alpha cell must undergo reductional division to return the state. Despite presence several "meiosis-specific" genes in C. genome, meiotic program not been observed. Instead, products can be induced random chromosome loss, often producing strains are diploid, or close ploidy. Using SNP comparative genome hybridization arrays we have now...
Influenza vaccines must be updated regularly because influenza viruses continuously acquire mutations in antibody binding sites of hemagglutinin (HA). The majority H3N2 strains circulating the Northern Hemisphere during 2014–2015 season are antigenically mismatched to A/Texas/50/2012 vaccine strain. Recent possess several new HA mutations, and it is unknown which these contribute mismatch. Here, we use reverse genetics demonstrate that antigenic site B primarily responsible for current Sera...
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is an emerging technology for rapid identification bacterial and fungal isolates. In comparison to conventional methods, this much less labor intensive can provide accurate reliable results in minutes from a single isolated colony. We compared the cost performing bioMérieux Vitek MALDI-TOF MS with microbiological methods determine amount saved by laboratory converting new technology. Identification...
Sexual reproduction can promote genetic diversity in eukaryotes, and yet many pathogenic fungi have been labeled as obligate asexual species. It is becoming increasingly clear, however, that cryptic sexual programs may exist some species, efficient mating requires the necessary developmental switch to be triggered. In this study we investigate Candida tropicalis , an important human fungal pathogen has reported asexual. Significantly, demonstrate C. uses a phenotypic regulate program of...
Candida albicans is both a common commensal and an opportunistic pathogen, being prevalent cause of mucosal systemic infections in humans. Phenotypic switching between white opaque forms reversible transition that influences virulence, mating behavior, biofilm formation. In this work, we show wide range factors induces high rates from to opaque. These include different environmental stimuli such as genotoxic oxidative stress, well intrinsic mutations DNA repair genes. We propose these...
ABSTRACT We evaluated the performance of Bruker Biotyper and bioMérieux Vitek MS with both SARAMIS v4.09 Knowledge Base v2.0 databases for identification 203 non-glucose-fermenting Gram-negative rods that had previously been identified by 16S rRNA gene sequencing. Including those underwent repeat testing, 96.6%, 90.1%, 93.6% isolates, respectively, identifications agreed previous identification.
INTRODUCTIONSyndromic panels were first FDA cleared for detection of respiratory pathogens in 2008. Since then, other have been approved by the FDA, and most recently, FilmArray meningitis/encephalitis panel (BioFire, Salt Lake City, UT) has become available. This assay detects 14 targets within 1 h includes that typically cause different manifestations infection, although they infect same organ system. Several studies reported both false-positive false-negative results with this test, all...
Abstract Background Patient room sinks are frequently identified as potential sources for hospital-associated infections (HAIs), but there no clear recommendations interventions to reduce sink-related pathogenic bioburden. We conducted one of the first longitudinal crossover trials assess impact practical sink in an academic hospital.Figure 1.Contamination levels patient p-trap and tap water between units over time.Black lines represent samples with CFU/mL above limit detection. Methods a...
Abstract Background Extended-spectrum cephalosporin-resistant Enterobacterales (ESCrE) are a major global threat, and there is significant gap in research on the burden associated risk factors for ESCrE low-and middle income countries(LMICs). This particularly true people with HIV (PWH), who make up proportion of population sub-Saharan Africa. In Botswana, 20% individuals aged 15-49 PWH. The colonization, typically precursor to infection, important understand as such infections can result...
Abstract Background The epidemiology of colonization with ESCrE in the community low- and middle-income countries (LMICs) is largely uncharacterized. In community, household particular importance as it setting which individuals spend a majority their time. Identifying risk factors for critical to inform antibiotic resistance reduction strategies LMICs. Methods Participants were enrolled from 1/1/20-9/4/20, pause 4/2/20-5/21/20 due country-wide COVID lockdown. This study was conducted 6...
The opportunistic pathogen Candida albicans undergoes a parasexual mating cycle in which cells must switch from the conventional “white” form to alternative “opaque” become competent. Pheromones secreted by opaque induce formation of polarized projections and result cell–cell conjugation. In contrast, white are unable undergo mating, but can still respond pheromone expression adhesion genes that promote biofilm formation. this study, we have analyzed dual ability pheromones activate cells....
Using retrospective (n = 200) and prospective 150) nasopharyngeal specimens, we evaluated the Nanosphere Verigene RV+ Focus Diagnostics Simplexa Flu A/B & RSV tests. Overall, demonstrated sensitivities specificities of 96.6% 100% for influenza A virus, 99.7% B respiratory syncytial virus (RSV), while test were 82.8 99.7%, 76.2 100%, 94.6 respectively.
OBJECTIVE An improved understanding of carbapenem-resistant Klebsiella pneumoniae (CRKP) in long-term acute care hospitals (LTACHs) is needed. The objective this study was to assess risk factors for colonization or infection with CRKP LTACH residents. METHODS A case-control performed at a university-affiliated from 2008 2013. Cases were defined as all patients clinical cultures positive and controls those carbapenem-susceptible K. (CSKP). multivariate model developed identify colonization....
Treatment options for Achromobacter xylosoxidans are limited. Eight cystic fibrosis patients with A. were treated 12 cefiderocol courses. Pretreatment in vitro resistance was seen 3 of 8 cases. Clinical response occurred after 11 treatment However, microbiologic relapse observed courses, notably without emergence resistance.
Abstract Background Approximately 40% of all Enterobacterales (EB) bloodstream infections (BSIs) among solid organ transplant recipients (SOTRs) are due to extended-spectrum β-lactamase (ESBL)–producing organisms, but risk factors for such remain ill defined in this population. We sought determine the ESBL-EB BSIs SOTRs. Methods A multicenter case-control study was performed. All SOTRs with an EB BSI at Hospital University Pennsylvania and Maryland Medical Center between 1 January 2007 30...
Although extended-spectrum cephalosporin-resistant Enterobacterales (ESCrE) and carbapenem-resistant (CRE) are a global challenge, data on these organisms in low- middle-income countries limited. In this study, we sought to characterize colonization critical for greater antibiotic resistance surveillance efforts.This study was conducted three hospitals six clinics Botswana. We ongoing of adult patients adults children the community. All participants underwent rectal swab sampling identify...
In 2022, the Clinical and Laboratory Standards Institute (CLSI) updated piperacillin-tazobactam (TZP) breakpoints for
ABSTRACT Implementation of the Verigene Gram-positive blood culture test led to reductions in time acceptable antibiotic overall (1.9 versus 13.2 h, respectively; P = 0.04) and appropriate for patients with vancomycin-resistant Enterococcus (4.2 43.7 h; 0.006) viridans group Streptococcus (0.2 7.1 0.02).
Seventy-eight blood cultures with a Gram stain result of Gram-positive cocci in pairs and/or chains were evaluated the Nanosphere Verigene culture (BC-GP) assay. The overall concordance assay was 89.7% (70/78 cultures), allowing for development targeted treatment algorithm.