A5031330858- Gut microbiota and health
- Trauma and Emergency Care Studies
- Clostridium difficile and Clostridium perfringens research
- Enhanced Recovery After Surgery
- Hernia repair and management
- Diet and metabolism studies
- SARS-CoV-2 and COVID-19 Research
- Injury Epidemiology and Prevention
- Intestinal and Peritoneal Adhesions
- COVID-19 Clinical Research Studies
- Emergency and Acute Care Studies
- MicroRNA in disease regulation
- Intestinal Malrotation and Obstruction Disorders
- Clinical Nutrition and Gastroenterology
- Intensive Care Unit Cognitive Disorders
- Gun Ownership and Violence Research
- Abdominal Trauma and Injuries
- Breast Implant and Reconstruction
- Long-Term Effects of COVID-19
- Gastrointestinal motility and disorders
- BRCA gene mutations in cancer
- Breast Cancer Treatment Studies
- Cardiac Arrest and Resuscitation
- Colorectal Cancer Surgical Treatments
- Lymphoma Diagnosis and Treatment
University of Chicago
2017-2023
United States Congress
2022
University of Chicago Medical Center
2021-2022
Comer Children's Hospital
2021
Florida College
2020
University of Florida
2020
University of Miami
2020
University of Colorado Denver
2020
American Association for the Surgery of Trauma
2020
RELX Group (Netherlands)
2020
Death due to sepsis remains a persistent threat critically ill patients confined the intensive care unit and is characterized by colonization with multi-drug-resistant healthcare-associated pathogens. Here we report that in mice caused defined four-member pathogen community isolated from patient lethal associated systemic suppression of key elements host transcriptome required for clearance decreased butyrate expression. More specifically, these pathogens directly suppress interferon...
Abstract We investigated the role of microRNA-21 in macrophage response to peritonitis; expression increases peritoneal macrophages after lipopolysaccharide stimulation but is delayed until 48 hours cecal ligation and puncture. MicroRNA-21–null mice bone marrow–derived cell lines were exposed puncture or lipopolysaccharide, survival, levels, target messenger RNAs proteins, cytokines assayed. Macrophages also transfected with mimics antagomirs, similar endpoints measured. Survival...
Abstract Background Both obesity and the presence of collagenolytic bacterial strains (Enterococcus faecalis) can increase risk anastomotic leak. The aim this study was to determine whether mice chronically fed a high-fat Western-type diet (WD) develop leak in association with altered microbiota, be mitigated by short course standard chow (SD; low fat/high fibre) before surgery. Methods Male C57BL/6 were assigned either SD or an obesogenic WD for 6 weeks followed preoperative antibiotics...
Convalescent plasma therapy for COVID-19 relies on transfer of anti-viral antibody from donors to recipients via transfusion. The relationship between clinical characteristics and response is not well defined. We investigated predictors convalescent production quantified recipient in a trial.
Obesity remains a prevalent and independent risk factor for life-threatening infection following major surgery. Here, we demonstrate that when mice are fed an obesogenic Western diet (WD), they become susceptible to lethal sepsis with multiple organ damage after exposure antibiotics otherwise-recoverable surgical injury. Analysis of the gut microbiota in this model demonstrates WD alone leads loss Bacteroidetes , bloom Proteobacteria evidence antibiotic resistance development even before...
Aims: To define the number/frequency of organ systems affected by extraintestinal manifestations (EIMs), to identify factors affecting clinical course inflammatory bowel disease (IBD) and EIM development, determine impact smoking, duration location on diagnosis EIMs in Crohn's (CD) ulcerative colitis (UC). Methods: IBD patients were derived from a single university colorectal surgery practice. Smoking data obtained through modified Behavioral Risk Factor Surveillance System survey. The...
Objectives: Determine whether preoperative dietary prehabilitation with a low-fat, high-fiber diet reverses the impact of Western (WD) on intestinal microbiota and improves postoperative survival Background: We have previously demonstrated that WD fed mice subjected to an otherwise recoverable surgical injury (30% hepatectomy), antibiotics, short period starvation demonstrate reduced (29%) compared standard chow (SD) (100%). Methods: Mice were 6 weeks underwent pre-habilitation (3 days vs 7...
The cecum is a unique region in the mammalian intestinal tract which microbiome localized to two compartments, lumen and crypts. within crypts particularly important as it direct contact with lining epithelial cells including stem cells. Here, we analyzed of mice using multiple techniques metagenomics. comprised Firmicutes Bacteroidetes whereas were dominated by Proteobacteria Deferribacteres, mucus mixture these 4 phyla. microbial functional potential mainly carbon metabolism, while crypt...
Shock index, pediatric age adjusted (SIPA), has been widely applied in trauma but limited precision because of the reference ranges used its derivation. We hypothesized that a shock index (PSI) equation based on age-based vital signs would outperform SIPA.A retrospective cohort patients aged 1 to 18 years from Trauma Quality Programs - Participant Use File 2010 2018 was performed. A random 70% training subset derive Youden index-optimizing (SI) cutoffs by for blood transfusion within 4...
Small bowel obstruction is a common clinical occurrence, primarily caused by adhesions. The diagnosis usually made on the findings and presence of dilated loops plain abdominal radiograph. Computed tomography (CT) increasingly used to diagnose cause location aid in timing surgical intervention. We retrospective chart review identify patients with small between 2009 2012. compared CT at operative Sixty had subsequent Eighty-three per cent CTs were correct for intestine involvement 80 colon...
Background: Anastomotic leak is among the most dreaded complications in patients undergoing colorectal surgery. We have discovered that rodents, collagenase-producing bacteria, particularly Enterococcus faecalis, promotes anastomotic by degrading healing tissue. Yet, it unclear if these organisms play a role humans. Patients and Methods: resection at University of Chicago from July 2014 through June 2019 who developed post-operative infection were stratified into infections resulted an leak,...
ABSTRACT Background Convalescent plasma therapy for COVID-19 relies on the transfer of anti-viral antibody from donors to recipients via transfusion. The relationship between clinical characteristics and response is not well defined. We investigated predictors convalescent production quantified recipient in a trial. Methods Multivariable analysis serological parameters 103 confirmed 28 days or more following symptom resolution was performed. Mixed effects regression models with piecewise...
Objective: To determine the efficacy of an orally delivered phosphate-rich polymer, Pi-PEG, to prevent surgical site infection (SSI) in a mouse model spontaneous wound involving gut-derived pathogens. Background: Evidence suggests that pathogens originating from gut microbiota can cause postoperative via process by which they silently travel inside immune cell and contaminate remote operative (Trojan Horse Hypothesis). Here, we hypothesize Pi-PEG SSIs novel mice. Methods: Mice were fed...
ABSTRACT Background Convalescent plasma therapy for COVID-19 relies on the transfer of anti-viral antibody from donors to recipients via transfusion. The relationship between clinical characteristics and response is not well defined. We investigated predictors convalescent production quantified recipient in a trial. Methods Multivariable analysis serological parameters 103 confirmed 28 days or more following symptom resolution was performed. Mixed effects regression models with piecewise...
Background: Convalescent plasma is a potential therapy for COVID-19. Using existing resources at single academic medical center, we established an integrated convalescent treatment and antibody research program to treat patients with severe COVID-19 advance vaccine design.Methods: We redeployed hospital infrastructure personnel conduct open label clinical trial hospitalized patients. Confirmed donors were recruited 28 days following symptom resolution. Whole blood donation occurred our...
The equivalent Injury Severity Score (ISS) cutoffs for severe trauma vary between adult (ISS, >16) and pediatric >25) trauma. We hypothesized that a novel injury severity prediction model incorporating age mechanism of would outperform standard ISS cutoffs.The 2010 to 2016 National Trauma Data Bank was queried patients. Cut point analysis used determine the optimal predicting mortality injury. Linear discriminant implemented accuracy, based on area under curve (AUC), cutoff 25 25), shock...
The United States has the highest per-capita incarceration rate and largest prison population in world. More than two thirds of recently incarcerated individuals will be arrested again within 3 years release may commit crimes as serious homicide soon after discharge. pattern homicidal violence currently remains unknown for suspects (RIHS) their victims.A retrospective analysis 36 states included 2003 to 2017 National Violent Death Reporting System was performed with a focus on RIHS victims....