A5033527033- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Pluripotent Stem Cells Research
- Nuclear Receptors and Signaling
- Vitamin D Research Studies
- Neuropeptides and Animal Physiology
- Xenotransplantation and immune response
- Signaling Pathways in Disease
- Mesenchymal stem cell research
- Tissue Engineering and Regenerative Medicine
- Inflammatory Bowel Disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Stress Responses and Cortisol
- Vitamin C and Antioxidants Research
- Gastrointestinal motility and disorders
- Retinoids in leukemia and cellular processes
- Congenital gastrointestinal and neural anomalies
- Helicobacter pylori-related gastroenterology studies
- Endoplasmic Reticulum Stress and Disease
- Peroxisome Proliferator-Activated Receptors
- Anesthesia and Neurotoxicity Research
- Aldose Reductase and Taurine
- Whipple's Disease and Interleukins
- Animal Genetics and Reproduction
Inserm
2006-2024
Nantes Université
2006-2024
The Enteric Nervous System in Gut and Brain Disorders
2016-2024
Centre Hospitalier Universitaire de Nantes
2001-2019
Centre de Recherche en Nutrition Humaine Rhône-Alpes
2016
PhysioPathologie des Adaptations Nutritionnelles
2016
3M (United States)
2016
Institut de Transplantation Urologie en Nephrologie
2001-2014
Université Nantes Angers Le Mans
2014
Centre d'Investigation Clinique de Nantes
2008
Abstract 1,25‐dihydroxyvitamin D 3 (1,25‐(OH) 2 ), a metabolically active form of vitamin D, is shown to increase in dose‐dependent manner the cellular pool NGF mRNA murine L‐929 fibroblasts cultured serum‐free medium. This effect can be detected as early hours after 1,25‐(OH) addition and persists for at least 28 hours. It accompanied by an enhancement amount NGF‐protein secreted culture Since proto‐oncogene c‐fos appears involved regulation gene (Mocchetti et al.: Proceedings National...
Glial cell line-derived neurotrophic factor (GDNF) has significant therapeutic potentials, in particular for neurodegenerative disorders. To determine factors that would enhance GDNF expression, we analysed the effect of 1,25-(OH)2 D3 C6 glioma cells. Treatment cells with 10−7 M, 48 h elicited an 18.5-fold increase level mRNA. In addition, our results indicate is effective at concentrations as low 10−10 M and retinoic acid additive effects. These data a potent inducer expression suggest may...
The development of cerebellar cortex is strongly impaired by thyroid hormone (T3) deficiency, leading to altered migration, differentiation, synaptogenesis, and survival neurons. To determine whether alteration in the expression neurotrophins and/or their receptors may contribute these impairments, we first analyzed using a sensitive RNAse protection assay situ hybridization; second, administered deficient hypothyroid animals. We found that early hypothyroidism disrupted developmental...
THE effect of 1,25-dihydroxyvitamin D3 on neurotrophin mRNA expression was studied in primary cultures astrocytes. In addition to its known effects NGF expression, 1,25-dihydroxy vitamin shown upregulate NT-3 levels, while NT-4 slightly but significantly downregulated. No observed BDNF expression. These data clearly show a differential regulation the four neurotrophins by astrocytes, and suggest that may participate NGF, NT-3, central nervous system.
It remains unknown what causes inflammatory bowel disease (IBD), including signaling networks perpetuating chronic gastrointestinal inflammation in Crohn's (CD) and ulcerative colitis (UC), humans. According to an analysis of up 500 patients with IBD 100 controls, we report that key transcripts the IL-7 receptor (IL-7R) pathway are accumulated inflamed colon tissues severe CD UC not responding either immunosuppressive/corticosteroid, anti-TNF, or anti-α4β7 therapies. High expression both...
The rev-erbA(alpha) gene, belonging to the steroid receptor superfamily of transcription factors, is highly conserved during evolution but little known so far about its functions in development or adult physiology. Here, we describe genetically altered mice lacking gene. These animals do not show any obvious phenotype either fat tissue skeletal muscle, despite regulation expression adipocyte and myotube differentiation vitro. However, second week life, cerebellum mutants presents several...
Cultured microglial cells were examined for their ability to metabolize 25-hydroxyvitamin D3 (25-(OH) D3). Upon exposure lipopolysaccharide, produced a vitamin D metabolite which comigrated with synthetic 1,25-dihydroxyvitamin (1,25-(OH)2D3) in two different systems of high performance liquid chromatography. This had the same affinity as 1,25-(OH)2D3 chick intestinal receptor. Lipopolysaccharide-stimulated incubated 3 nM 25-(OH) synthesized up 5.76 fmol 1,25-(OH)2D3/8 x 10(5) cells/2 hr....
Neural transplantation is a promising therapeutic strategy for neurodegenerative diseases and other disorders of the central nervous system (CNS) such as Parkinson Huntington diseases, multiple sclerosis or stroke. Although cell replacement therapy already went through clinical trials some these using fetal human neuroblasts, several significant limitations led to search alternative sources that would be more suitable intracerebral transplantation.Taking into account logistical ethical...
The presence of myenteric plexitis in the proximal resection margins is a predictive factor early postoperative recurrence Crohn's disease. To decipher mechanisms leading to their formation, T-cell interactions with enteric neural cells were studied vitro and vivo. T close retrospectively quantified ileocolonic resections from 9 control subjects cancer 20 patients involved adhesion then investigated co-cultures lymphocytes glial (glia). Finally, implication molecules development colitis was...
The vitamin D receptor (VDR) is a nuclear that mediates the effect of active metabolite D3, 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3). To investigate potential role this hormone in peripheral nervous system, we have studied VDR expression Schwann cells. mRNA was detected by Northern blot analysis rat primary cultures cells, and its levels were strongly increased presence 1,25-(OH)2D3. Using mouse cell line, MSC80, showed concentrations as low 10(-10) M stimulated gene amounts activated VDR,...
Receptor serine-threonine kinases (RSTK) mediate inhibitory as well stimulatory signals for growth and differentiation by binding to members of the transforming factor-β (TGF-β) superfamily. Over 12 different RSTKs have been isolated so far, displaying wide expression in peripheral tissues nervous system. Here we report isolation characterization a novel type I RSTK termed activin receptor-like kinase-7 (ALK-7) that, unlike other this receptor family, is predominantly expressed adult central...
A transgenic Sprague Dawley rat bearing the A30P and A53T α-synuclein (α-syn) human mutations under control of tyrosine hydroxylase promoter was generated in order to get a better understanding role α-syn on neuropathological events involved progression Parkinson's disease (PD). This displayed olfactory deficits absence motor impairments as observed most early PD cases. In investigate mutated cell proliferation, we focused subventricular zone (SVZ) bulbs (OB) change proliferation could...
The postnatal period is crucial for the development of gastrointestinal (GI) functions. enteric nervous system a key regulator GI functions, and increasing evidences indicate that 1) maturation neurons affect 2) microbiota-derived short-chain fatty acids can be involved in this maturation. Although glial cells (EGC) are central regulators evolution their phenotype remains poorly defined. We thus characterized EGC colon rat pups studied effect on showed an increased expression markers GFAP...
Abstract: Newborn rat brain astrocytes cultured in vitro a chemically defined medium are shown to secrete enhanced levels of nerve growth factor (NGF) when they exposed various types proteases. Proteolytic enzymes such as α‐thrombin or collagenase induce continuous, dose‐dependent enhancement the cell‐secreted NGF. Incubation for 24‐h period with 300 ng/ml (∼9 n M , 1 U/ml) results an increase NGF by three‐ fourfold, and at doses 10 times higher, stimulation up four‐to fivefold was observed....
The expression of the 25(OH) vitamin D3 24-hydroxylase gene was studied in C6 glioma and rat primary glial cell culture. 25(OH)D3 not detected or cells cultured a serum-free medium. However, mRNA induced dose-dependent manner treated with 1,25(OH)2D3. These findings provide further evidence for an involvement metabolites brain function.
Abstract Nestin, a currently used marker of neural stem cells, is transiently coexpressed with glial fibrillary acidic protein (GFAP) during development and induced in reactive astrocytes following brain injury. Nestin expression has also been found cultures astroglial but little known about the fate mitotic activity nestin‐expressing cells this vitro model. The present study reveals long‐lasting nestin primary derived from rat brain. Over 70% were + at 12 weeks, large majority coexpressing...
Neural transplantation is a promising therapeutic approach for neurodegenerative diseases; however, many patients receiving intracerebral fetal allografts exhibit signs of immunization to donor antigens that could compromise the graft. In this context, we intracerebrally transplanted mesencephalic pig xenografts into primates identify suitable strategy enable long-term cell survival, maturation, and differentiation. Parkinsonian received WT or CTLA4-Ig transgenic porcine different durations...