A5066704514- Tissue Engineering and Regenerative Medicine
- Cardiac Imaging and Diagnostics
- Cardiac electrophysiology and arrhythmias
- Advanced MRI Techniques and Applications
- Cardiac Ischemia and Reperfusion
- Electrospun Nanofibers in Biomedical Applications
- Cardiovascular Function and Risk Factors
- Mesenchymal stem cell research
- Cardiac Fibrosis and Remodeling
- Eicosanoids and Hypertension Pharmacology
- Cardiovascular Disease and Adiposity
- Cardiomyopathy and Myosin Studies
- Adipose Tissue and Metabolism
- Cardiac pacing and defibrillation studies
- Cardiac Arrhythmias and Treatments
- Heart Rate Variability and Autonomic Control
- Cardiovascular Effects of Exercise
- Nitric Oxide and Endothelin Effects
- Fatty Acid Research and Health
- Air Quality and Health Impacts
- Heat shock proteins research
- Electron Spin Resonance Studies
- Cholesterol and Lipid Metabolism
- Ion channel regulation and function
- Angiogenesis and VEGF in Cancer
University of California, San Francisco
2011-2020
California Institute for Regenerative Medicine
2019-2020
Third Hospital of Hebei Medical University
2015
Hebei Medical University
2015
Broad Center
2010
Ludwig Cancer Research
2008
University of Helsinki
2008
Ludwig Cancer Research
2008
Goethe University Frankfurt
2008
KU Leuven
2008
Current efforts in cardiac tissue engineering center around the use of scaffolds that deliver cells to epicardial surface. In this study, we examined effects fibrin glue as an injectable scaffold and wall support ischemic myocardium. The left coronary artery rats was occluded for 17 min, followed by reperfusion. Echocardiography performed 8 days after infarction. One 2 later, either 0.5% bovine serum albumin (BSA) phosphate-buffered saline, alone, skeletal myoblasts or were injected into...
Efficacy of potential treatments for myocardial infarction (MI) is commonly assessed by histological measurement infarct size in rodent models. In experiments involving an acute MI setting, the infarcted area tissue sections left ventricle a standard approach to determine size. This has also been used chronic setting measure several weeks post-MI. We tested hypothesis that, because wall thinning known occur would be less appropriate. compared measurements based on 1) area, 2) epicardial and...
BACKGROUND Previous studies have demonstrated that heat-shock treatment results in the induction of 72-kD protein (HSP72) and a reduction infarct size after subsequent ischemia reperfusion. METHODS AND RESULTS To test hypothesis degree protection from ischemic injury heat-shocked rats correlates with prior HSP72 induction, pretreated 40 degrees C, 41 or 42 C whole-body hyperthermia followed by 24 hours recovery control (n = 6 each group) were quantitatively assessed for presence myocardial...
BACKGROUND To test the hypothesis that heat shock response is associated with improved myocardial salvage after ischemia and reperfusion, rats treated prior whole-body hyperthermia 24 hours of recovery (n = 26) or 20 minutes ischemic pretreatment 8 24) control 27, n 24, for hyperthermic pretreatment, respectively) were subjected to 35 left coronary artery (LCA) occlusion 120 reperfusion. METHODS AND RESULTS Although ventricular samples from either 7) 6) all showed induction HSP72 (heat...
Background Previous studies have demonstrated that induction of heat shock protein (HSP) 72 by whole-body hyperthermia reduces infarct size in an vivo model ischemia and reperfusion. Furthermore, hearts obtained from transgenic mice overexpress HSP72 demonstrate improved functional recovery decreased vitro after global Methods Results To test the hypothesis overexpression vivo, were heterozygous for a rat HSP70i gene ([+]HSP72) transgene-negative littermate controls ([−]HSP72) subjected to...
Although previous investigators have demonstrated that myocardial preconditioning reduces infarct size, the mechanisms of cardioprotection associated with are not completely understood.To test hypothesis (four 5-minute episodes ischemia each followed by 5 minutes reperfusion) size depleting cardiac glycogen stores and attenuating degree intracellular acidosis during subsequent prolonged left coronary artery occlusion, preconditioned control rats were subjected to 45 occlusion 120 reflow...
Previous studies have demonstrated that ischemic preconditioning prevents lethal cell injury and, as a consequence, limits infarct size in rat heart. Although both apoptosis and necrosis been shown to contribute myocardial death after ischemia reperfusion, the ability of prevent programmed remains unknown.To test hypothesis reduces irreversible part by decreasing apoptosis, rats underwent controls were subjected 30 minutes left coronary artery occlusion followed 180 reperfusion. Ischemic was...
Novel strategies by which to repair ischemic myocardium after myocardial infarction include the use of three-dimensional polymer scaffolds. A comparative study was carried out assess therapeutic potential fibrin, collagen I, and Matrigel as injectable biopolymers for infarction. Using a rat model left coronary artery occlusion followed reperfusion, local injection into infarct zone yielded significantly higher levels capillary formation, when compared with saline control group, at 5 weeks...
Acute myocardial infarctions were produced in 11 dogs by ligation of the left anterior descending coronary artery. Twenty-four hours after ligation, 0.35 millimoles per kilogram Gd-DTPA was injected intravenously, followed cardiectomy either 90 seconds (3 dogs) or 5 minutes (5 later. The remaining 3 had without injection at 24 occlusion. that did not receive longer T1 and T2 relaxation times infarcted myocardium than normal myocardium, as measured a 10.7-MHz magnetic resonance (MR)...
Despite public awareness that tobacco secondhand smoke (SHS) is harmful, many people still assume marijuana SHS benign. Debates about whether smoke-free laws should include are becoming increasingly widespread as legalized and the cannabis industry grows. Lack of evidence for causing acute cardiovascular harm frequently mistaken it harmless, despite chemical physical similarity between smoke. We investigated brief exposure to causes vascular endothelial dysfunction.
Abstract Intramyocardial injection of hydrogels offers great potential for treating myocardial infarction (MI) in a minimally invasive manner. However, traditional bulk generally lack microporous structures to support rapid tissue ingrowth and biochemical signals prevent fibrotic remodeling toward heart failure. To address such challenges, novel drug‐releasing annealed particle (drugMAP) system is developed by encapsulating hydrophobic drug‐loaded nanoparticles into microgel building blocks...
Gadolinium-DTPA (Gd-DTPA) was used to improve the diagnostic utility of magnetic resonance (MR) in detecting early ischemia, before onset infarction. Following one minute left anterior descending coronary artery occlusion, 9 dogs were intravenously injected with either 0.5 mM/kg Gd-DTPA (6 dogs) or normal saline (3 dogs). One more allowed for perfusion injectate prior cardiectomy and MR imaging ex situ heart. There no visible difference intensity alterations relaxation times between ischemic...
The influence of atrial systole on the left ventricular function curve (stroke volume vs end-diastolic pressure or diameter) and diameter relation was studied in nine anesthetized, open-chest dogs whose atrioventricular (AV) node had been completely blocked. Measurements were made during loading with pericardium closed opened alternate AV sequential pacing (to permit contribution to filling) simultaneous prevent contribution). When closed, withdrawal shifted stroke-volume downward, but did...
Objective— The endogenous role of the VEGF family member vascular endothelial growth factor-B (VEGF-B) in pathological angiogenesis remains unclear. Methods and Results— We studied VEGF-B various models using mice lacking (VEGF-B −/− ) or overexpressing 167 . After occlusion left coronary artery, deficiency impaired vessel ischemic myocardium whereas, wild-type mice, overexpression enhanced revascularization infarct border zone. By contrast, did not affect wounded skin, hypoxic lung, retina,...