A5086555388- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Parasites and Host Interactions
- Immune responses and vaccinations
- Parasite Biology and Host Interactions
- Diagnosis and treatment of tuberculosis
- Immune cells in cancer
- Immune Cell Function and Interaction
- Pneumocystis jirovecii pneumonia detection and treatment
- Parasitic Diseases Research and Treatment
- Child Nutrition and Water Access
- Diabetes and associated disorders
- Helminth infection and control
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Iron Metabolism and Disorders
- Vector-Borne Animal Diseases
- Immune Response and Inflammation
- Immunodeficiency and Autoimmune Disorders
- Viral gastroenteritis research and epidemiology
- Cytokine Signaling Pathways and Interactions
- Infectious Diseases and Mycology
- Immunotherapy and Immune Responses
- Pharmacological Effects of Natural Compounds
Kumasi Centre for Collaborative Research in Tropical Medicine
2020-2024
Heinrich Heine University Düsseldorf
2024
Düsseldorf University Hospital
2024
Kwame Nkrumah University of Science and Technology
2022-2024
Kwame Nkrumah University
2024
University of Cape Coast
2022
Mycobacterium (M.) tuberculosis-caused immunopathology is characterized by aberrant expression of plasma cytokines in human tuberculosis. Disease severity and long-term anti-mycobacterial treatment are potentially influenced normalization cytokine levels during therapy may indicate efficacy recovery.In this study, we analyzed the concentrations selected (i.e., IL-6, IP-10, IL-10, IL-22, IFNγ, GM-CSF, IL-8) M. tuberculosis sputum burden patients with (n = 76). Cytokine were compared to...
Abstract Purpose Human tuberculosis is characterized by immunopathology that affects T-cell phenotype and functions. Previous studies found impaired response to phytohemagglutinin (PHA) in patients with acute tuberculosis. However, the influence of disease severity, affected subsets, underlying mechanisms remain elusive. Methods Here we investigated PHA-induced antigen-specific effector cytokines ( n = 55) as well healthy asymptomatic contacts 32) from Ghana. Effects Mycobacterium (M.)...
Altered monocyte differentiation and effector functions characterize immune pathogenesis of tuberculosis. IL-7 is an important factor for proliferation T cells impaired sensitivity due to decreased receptor α-chain (IL-7Rα) expression was found in patients with acute Peripheral blood monocytes have moderate IL-7Rα increased levels were described inflammatory diseases. In this study, we investigated a potential role We analyzed the phenotype from tuberculosis (n = 33), asymptomatic contacts...
Abstract Monocyte‐derived macrophages contribute centrally to immune protection in Mycobacterium tuberculosis infection and changes monocyte phenotype characterize immunopathology patients. Recent studies highlighted an important role of the plasma milieu immunopathology. Here, we investigated pathology patients with acute determined effects on as well cytokine signalling reference monocytes. Patients ( n = 37) asymptomatic contacts (controls 35) were recruited part a hospital‐based study...
Mycobacterium (M.) bovis BCG vaccination is recommended for healthy babies after birth in several countries with a high prevalence of tuberculosis, including Ghana. Previous studies showed that prevents individuals from developing severe clinical manifestations but effects on the induction IFN-γ M. tuberculosis infection have hardly been investigated. Here, we performed IFN-γ-based T-cell assays (i.e., Release Assay, IGRA; activation and maturation marker assay, TAM-TB) children who had...
Bacterial components and cytokines induce IL-7 receptor (IL-7Rα) expression in monocytes. Aberrant low IL-7Rα of monocytes has been identified as a feature tuberculosis immunopathology. Here, we investigated the mechanisms underlying regulation serum effects on expression. Serum samples from patients healthy controls, cytokine candidates, mycobacterial were analyzed for vitro primary monocytes, monocyte-derived macrophages (MDM), monocyte cell lines. during culture role FoxO1 characterized....
Abstract Impaired T-cell responses to mitogens and high activation marker (TAM) expression on Mycobacterium tuberculosis –specific T-cells characterize immunopathology in patients with (TB). In a study of TB ( n = 60) asymptomatic contacts (controls, 37), we found that had higher CD38 + proportions specific for M. protein (PPD Mtb ), yet total PPD -specific were comparable. Notably, both activated (CD38 ) IFN-γ from lower mitogen (phytohemagglutinin, PHA)-induced responses. This impaired...
Abstract Background Culicoides , also known as biting midges, carry pathogens which include Mansonella perstans . is a nematode parasite implicated in number of disease outcomes. Even though high prevalence about 75% M. infection has been recorded some communities the middle belt Ghana, and wide diversity species identified, exact transmitting Ghana not yet deciphered. This study therefore aimed at assessing their role transmission Ghana. Methods were sampled from 11 Asante-Akim North Sene...
Background: According to the WHO, neglected tropical diseases (NTDs) affect over 2 billion people worldwide. While links between nutrition and many have become clear recent decade, NTDs lagged behind linkage with is largely unknown. We conducted this systematic review meta-analysis determine current status of research on role diet in pathogenesis NTDs. Methodology: PubMed, Embase, Scopus African Journal online databases were searched using predefined search terms. included all original...
BCG vaccination affects other diseases beyond tuberculosis by unknown—potentially immunomodulatory—mechanisms. Recent studies have shown that administered during overt type 1 diabetes (T1D) improved glycemic control and affected immune metabolic parameters. Here, we comprehensively characterized Ghanaian T1D patients with or without routine neonatal to identify vaccine-associated alterations. long-term (n = 108) matched healthy controls 214) were evaluated for disease-related clinical,...
Immunopathology of human tuberculosis (TB) in a subgroup patients is characterized by aberrantly high concentrations inflammatory cytokines, for example Interleukin (IL)-6. Concomitant (co-)infections parasites can affect host immunity, but the impact on immunopathology TB poorly defined. Here we group with ( n = 76) from Ghana different protozoan and helminth co-infections. Plasma cytokines were measured at onset disease anti-mycobacterial treatment efficacy was monitored during course. A...
Abstract Background and Aim Human hookworm disease caused by Ancylostoma duodenale Necator americanus is a serious public health problem. Hookworm infection activates eosinophil‐mediated tissue inflammatory responses, involving the production of eosinophil‐specific chemokine (eotaxin), recruitment eosinophils, secretion cationic protein, antiparasite immunoglobulin E (IgE). We investigated immune response as markers (CCL11, eosinophil protein [ECP], IgE) for detecting infection. Methods This...
Abstract Background Doxycycline is used for treatment of Mansonella perstans infection. Immune modulatory effects both M. and doxycycline have been described but long-term implications on host immune response are not defined. Here we determined multiple parameters perstans–infected individuals before after to characterize T-cell immunity. Methods characterization doxycycline-treated was performed as part an open-label randomized clinical trial. cell population phenotyping by flow cytometry...
Abstract Background Plasmodium falciparum and Hookworm infections are prevalent in West Africa they cause iron deficiency anemia protein malnutrition Children. Immune response of these parasites interact their interactions could have repercussions on vaccine development efficacy. The current goal hookworm eradication lies vaccination. We evaluated the effect P. coinfection albendazole treatment naturally acquired antibody profile against L3 stage larvae antigen. Methods In a longitudinal...
Abstract Background Buruli ulcer disease (BUD) caused by Mycobacterium (M.) ulcerans is characterized necrotic skin lesions. As for other mycobacterial infections, e.g., tuberculosis, the immune response important host protection. B-cells may play a role in antimycobacterial immunity but studies characterizing B-cell repertoire and memory generation BUD during course of treatment are scarce. Methods We investigated adaptive cell children with healthy matched controls flow cytometry. Analyses...
Background: Altered monocyte differentiation and effector functions characterize tuberculosis pathogenesis. Interleukin-7 (IL-7) mediated T-cell are impaired in acute patients IL-7 effects on monocytes hardly defined. Here we investigated receptor (IL-7Ra) expression of from charcterized the anti-mycobacterial immune response.Methods: We analyzed phenotype distribution peripheral blood subpopulations (n=33), latently Mycobacterium (M.) infected contacts (LTBI; n=30) healthy controls (n=20)...
Immune-based diagnosis of Buruli ulcer disease (BUD) in children is difficult due to cross-reactivity between mycobacteria. We found that T-cell IFNγ/TNFα responses against Mycobacterium (M.) ulcerans and M. tuberculosis (PPDMulc, PPDMtub) were different with BUD (n = 27) TB 20) but only ratios (PPDMtub/PPDMulc) discriminated the study groups efficiently.