A5102818140- Biochemical and Structural Characterization
- Protein Hydrolysis and Bioactive Peptides
- Antimicrobial Peptides and Activities
- Adsorption and biosorption for pollutant removal
- Geochemistry and Elemental Analysis
- Food Allergy and Anaphylaxis Research
- Immune Response and Inflammation
- Land Use and Ecosystem Services
- Urban Green Space and Health
- Insect Utilization and Effects
- Probiotics and Fermented Foods
- RNA and protein synthesis mechanisms
- Chromium effects and bioremediation
- Fibroblast Growth Factor Research
- Marine Bivalve and Aquaculture Studies
- Calcium Carbonate Crystallization and Inhibition
- Identification and Quantification in Food
- Wastewater Treatment and Nitrogen Removal
- Urban Agriculture and Sustainability
- Biochemical Analysis and Sensing Techniques
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Physiological and biochemical adaptations
- Occupational and environmental lung diseases
- Analytical chemistry methods development
- Aquaculture disease management and microbiota
Henan Agricultural University
2015-2025
Ocean University of China
2023-2024
Wangjing Hospital of China Academy of Chinese Medical Sciences
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2023
Xi'an University of Architecture and Technology
2019-2020
Under the fast-paced urban lifestyle, many residents suffer from sub-health. Forest-based health and wellness tourism is an effective way to relieve psychological physiological stress. Urban forest parks play a key role in construction. However, management of still unreasonable, so economic, social, ecological benefits these cannot be continuously utilized. In order manage perspective wellness, three categories indicators, namely visual sensitivity, characteristics, soil properties, were...
Antimicrobial peptides (AMPs) show high antibacterial activity against pathogens, which makes them potential new therapeutics to prevent and cure diseases. Porcine beta defensin 2 (pBD2) is a newly discovered AMP has shown different bacterial species including multi-resistant bacteria. In this study, the functional mechanism of pBD2 Staphylococcus aureus was investigated. After S. cells were incubated with concentrations pBD2, morphological changes in locations detected by electron...
Porcine beta defensin 2 (pBD2) is a cationic antimicrobial peptide with broad spectrum antibacterial activity, which makes it potential alternative to antibiotics prevent and cure diseases of pigs. However, development pBD2 as an effective antibiotic agent requires molecular understanding its functional mechanism against pathogens. In this study, we investigated the activity. Escherichia coli was incubated different concentrations for times. Electron microscopy used analyze locations induced...
Porcine β-defensin-2 (pBD2) is a cationic antimicrobial peptide that has therapeutic potential. The amount of pBD2 in nature limited, and the expression Escherichia coli low, probably because different gene codon used by prokaryotic organisms to eukaryotes. Codon preference optimization one ways increase heterologous pBD2. To achieve high pBD2, was redesigned according preferred E. without altering amino acid sequence. optimized inserted into vector pET-30a transformed BL21 (DE3) plysS. Our...
Fibrosis disrupts tissue balance and links to severe illnesses, impairing organ function and, in some cases, even fatality. The interaction between M2 macrophages fibroblasts is vital for equilibrium. Transforming growth factor β1 (TGF-β1) released by plays a central role fibrosis, regulating fibroblast activity extracellular matrix metabolism. Targeting TGF-β1 key fibrosis treatment. In our study using three cell lines, we reveal that the macrophage transcription SP1 enhances binding...
Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad activities against bacteria plays an important role in host defense. In order to enhance its activity better understand the effect of positively charged residues on activity, we substituted eight amino acid with arginine or lysine respectively. All mutants were cloned expressed BL21 (DE3) plysS mutant proteins then purified. These versions had higher positive charges but similar structural...
Crustacean shellfish are major allergens in East Asia. In the present study, a allergic protein crustaceans, tropomyosin, was detected accurately using multiple reaction monitoring mode-based mass spectrometry, with shared signature peptides identified through proteomic analysis. The were deliberately screened thermal stability and enzymatic digestion efficiency to improve suitability accuracy of developed method. Finally, proposed method demonstrated linear range 0.15 30 mg
The aim of this study is to explore differences in the peptidomics Saccharomyces pastorianus protein hydrolysates treated with different enzymes. Briefly, peptide fingerprints and active peptides neutral protease/papain-hydrolyzed S. were analyzed using ultra-high performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS) combined PEAKS Online 1.7 analysis software, Peptide Ranker, BIOPEP database. Compared traditional databases, uses de novo sequencing for obtain...