Abstract FOXP3 functions not only as the master regulator in regulatory T cells, but also an X-linked tumor suppressor. The tumor-suppressive activity of has been observed initiation, its role during progression remains controversial. Moreover, mechanism FOXP3-mediated largely unknown. Using chromatin immunoprecipitation (ChIP) sequencing, we identified a series potential FOXP3-targeted miRNAs MCF7 cells. Notably, significantly induced expression miR-146a/b. In vitro, FOXP3-induced...

Background: MicroRNAs (miRNAs) are small, single-stranded, noncoding RNAs, which usually bind to the 3'-untranslated region of target mRNAs and capable inducing posttranscriptional gene regulation by blocking translation or degrading mRNA. However, expression level miR-23 in radiation induced carcinogenesis is largely unknown. Methods: Radiation thymic lymphoma model BALB/c mice was set up. miR-23a & miR-23b miRNA levels different tissues cells were detected real-time qPCR. miR-23a/b...

Bone marrow mesenchymal stem cells (BMSCs)-derived exosomes are rich in a variety of active substances, including microRNA (miR) and have shown powerful therapeutic effects to ameliorate cell injury diseases. However, the role BMSCs-derived on chronic obstructive pulmonary disease (COPD) has been poorly studied. In addition, microvascular endothelial (PMVECs) apoptosis contributes onset COPD. Inhibition PMVECs can reverse COPD changes. Therefore, aim this study was explore investigate...

Abstract Radiation protection on male testis is an important task for ionizing radiation‐related workers or people who receive radiotherapy tumours near the testicle. In recent years, Toll‐like receptors (TLRs), especially TLR4, have been widely studied as a radiation target. this study, we detected that low‐toxicity TLR4 agonist monophosphoryl lipid A (MPLA) produced obvious effects mice testis. We found MPLA effectively alleviated structure damage and cell apoptosis induced by (IR)....

Abstract Background and Aim: N‐cadherin (N‐cad), one of the classic cadherins, has been reported to be involved in tumor metastasis some types tumors. This study aims investigate expression status N‐cad hepatocellular carcinoma (HCC) correlation between metastatic potential, as well surgical outcomes HCC. Methods: HCC adjacent liver tissues, normal was studied by immunohistochemistry Western blot, relationship clinicopathological features evaluated. By using RNA interference technique,...

The miR-200c has recently been implicated in the epithelial to mesenchymal transition (EMT) process by directly target EMT related transcriptional factors ZEB1 and ZEB2. expression of this miRNA is inversely correlated with tumorgenecity invasiveness several human cancers. However, little known about targets radiation carcinogenesis. Here study, using a split induced thymic lymphoma (RITL) model BALB/c mice, we found that down-regulated RITL samples. Cell death apoptosis cells was mimic...

Endometrial cancer (EC) ranks as the most prevalent malignancy occurring in female genital tract. Non-SMC condensin I complex subunit G (NCAPG), a mitotic associated chromosomal condensing protein, is reported to be frequently abnormally expressed several tumors and plays vital role carcinogenesis. Our study aimed explore effect of NCAPG on cell proliferation apoptosis EC cells determine underlying mechanism. Expression survival data tissues were analyzed by bioinformatics methods. Cell was...

Dysregulated follicular development may lead to atresia, and this is associated with oxidative stress in granulosa cells. Kurarinone a natural compound possessing multiple activities, including antioxidative ability. However, the role of kurarinone cell damage during atresia remains unknown. Human ovarian KGN cells were treated hydrogen peroxide (H2 O2 ) induce cellular damage. Cytotoxicity was investigated by lactate dehydrogenase (LDH) release assay. Oxidative evaluated detection reactive...

BACKGROUND Carbon ion radiotherapy has been shown to be more effective in cancer than photon irradiation. Influence of carbon radiation on microenvironment is very important for the outcomes radiotherapy. Tumor-infiltrating dendritic cells (DCs) play critical roles antigen processing and antitumor immunity. However, there scant literature covering effects DCs. In this study, we aimed uncover impact irradiation bone marrow derived MATERIAL AND METHODS Bone were co-cultured with GM-CSF IL-4...

RUNX1-IT1 suppresses colorectal cancer and liver cancer, while its role in other cancers is unknown. This study was performed to investigate the of endometrial (EC).EC paired non-tumor tissues were collected from 62 EC patients, expression RUNX1-IT1, mature miR-21 precursor these tissue samples determined by RT-qPCR. Correlations analyzed linear regression. Overexpression achieved cells CCK-8 assay used for cell proliferation analysis.We found that downregulated inversely correlated with but...

Oxidative stress injury and cell death in alveolar epithelial cells may lead to abnormal repair, further resulting acute chronic pulmonary diseases. Substance P (SP) has multiple biological activities. The Sonic hedgehog (SHH) pathway is important lung development decreasing injury. To investigate the effects of SP on type II (AEC IIs), AEC IIs were exposed 95% oxygen SHH signaling was examined. Primary isolated purified from premature rats. divided into four groups: air (21% oxygen) group,...

1. Wang C, Xu J, Yang L, et al. Prevalence and risk factors of chronic obstructive pulmonary disease in China (the Pulmonary Health [CPH] study): a national cross-sectional study. Lancet. 2018;391(10131):1706-1717. doi:10.1016/S0140-6736(18)30841-9 CrossRef Google Scholar

Although many pathological mechanisms and etiological hypotheses of acute unilateral vestibulopathy (AUVP) have been reported, but the actual etiology remains to be elucidated.This study was based on comprehensive bioinformatics identify critical genes AUVP explore its mechanism.Gene expression profiles normal samples were collected from GSE146230 datasets Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) constructed, WGCNA R-package extracted...

Ionizing radiation is one of the common environmental carcinogens. miRNAs play critical roles in processes tumor occurrence, development, metastasis. However, relationship between radiation-induced carcinogenesis and miRNA rarely reported. This study aimed to investigate effect on carcinogenesis. In this we established thymic lymphoma mice model. By using array RTL tissue predicting for target genes, a miRNA-mRNA crosstalk network was established. Based network, identified miRNA, miR-486,...

OBJECTIVE To investigate the effect of arsenic disulfide (AS2S2) combined with itraconazole on proliferation, apoptosis and hedgehog pathway diffuse large B-cell lymphoma (DLBCL) cells. METHODS The human DLBCL cell OCI-LY3 was treated different concentrations AS2S2 itraconazole. Cell proliferation inhibition detected by CCK-8, rate determined flow cytometry. expression levels BCL-2, BAX, SMO GLi1 were Western blot. RESULTS viability decreased significantly at 24, 48 or 72 h as cultured Along...

Abstract Background: Hematopoiesis and the differentiation of HSC have been proved to not only play important roles in cancer progression but also be changed or reprogrammed by tumor microenvironment itself. In this study, we investigated changes HSCs advanced tumor-bearing mice. Methods: The mice model was established subcutaneously inoculating with xenografts B16-F10 mouse melanoma cells into right back male wild-type C57BL/6 Hematopoietic stem multilineage were evaluated using blood...

Abstract Background: Radiation induced-intestinal injury (RIII) is a common complication after radiation therapy in patients with pelvic, abdominal or retroperitoneal tumors. The mechanism of RIII includes radiation-induced death intestinal epithelial cells (IECs) and damage stem (ISCs), among which ISCs main cause. Most recently, hypoxia Inducible factor (HIF) has been found to have effect on maintaining stemness promoting proliferation ISCs, suggests protective role HIF the RIII. In this...