A5101713381- Pharmacological Effects of Natural Compounds
- Blood Coagulation and Thrombosis Mechanisms
- Nigella sativa pharmacological applications
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Hemophilia Treatment and Research
- Protein Interaction Studies and Fluorescence Analysis
- Natural product bioactivities and synthesis
- Natural Antidiabetic Agents Studies
- Advanced Glycation End Products research
- Environmental Toxicology and Ecotoxicology
- Heavy Metal Exposure and Toxicity
- Platelet Disorders and Treatments
- Rheumatoid Arthritis Research and Therapies
- Blood properties and coagulation
- Bioactive Compounds and Antitumor Agents
- TGF-β signaling in diseases
- Musculoskeletal synovial abnormalities and treatments
- Saffron Plant Research Studies
- Protease and Inhibitor Mechanisms
- Phytochemicals and Medicinal Plants
- Advanced Drug Delivery Systems
- Pomegranate: compositions and health benefits
- Phytoestrogen effects and research
- Phytochemistry and biological activity of medicinal plants
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Noida International University
2023
Institute of Medical Sciences
2023
Jawaharlal Nehru Medical College Hospital
2012-2022
Aligarh Muslim University
2012-2022
Sarojini Naidu Medical College
2013
University of Lucknow
2013
Integral University
2013
Narsee Monjee Institute of Management Studies
2012
Temple University
1989-2011
Thrombosis Research Institute
2003
Nigella sativa belonging to the Ranunculaceae family has been reported use for thousands of years as protective and curative traditional medicine against a number diseases. GC-MS analysis methanolic extract (ME) volatile oil (VO) extracted from seed was performed by two different mass spectrometry libraries, WIlEY8 NIST05s. The cholesterol lowering antioxidant actions VO ME fractions were investigated in atherogenic suspension fed rats. In this study, four groups male Wistar rats used:...
When complex biological materials are analyzed without an adequate sample preparation technique, MS signal and response undergo significant alteration result in poor quantification assay. This problem generally takes place due to the presence of several endogenous component samples. One major causes ion suppression bioanalysis is phospholipids during LC-MS analysis. The phospholipid-based matrix effect was investigated with a commercially available electro spray ionization (ESI) source...
Abstract To investigate the activated platelet surface as a locus for factor X activation, functional consequences of IXa binding to platelets were studied. The concentration required half-maximal rates activation in presence VIIIa and thrombin-activated was 0.53 nM, which is close Kd (0.56 nM) under identical conditions, determined from equilibrium studies. In direct comparative experiments, there correspondence between kinetic determinations rates. Analysis by polyacrylamide gel...
This study aims to describe the therapeutic potential of C. nocturnum leaf extracts against diabetes and neurological disorders via targeting α-amylase acetylcholinesterase (AChE) activities, followed by computational molecular docking studies establish a strong rationale behind AChE inhibitory leaves-derived secondary metabolites. In our study, antioxidant activity sequentially extracted leaves extract was also investigated, in which methanolic fraction exhibited strongest DPPH (IC50 39.12...
Glycation is vital in terms of its damaging effect on macromolecules resulting the formation end products, which are highly reactive and cross-linked irreversible structures, known as advanced glycation products (AGEs). The continuous accumulation AGEs associated with severe diabetes ailments. Saccharides their reducing ends can glycate amino acid side chains proteins, among them glucose well-known for potent glycating capability. However, other sugars be more agents than glucose. D-ribose a...
In the present study, antioxidant efficacies of thymoquinone (TQ) and limonene (LMN), two main constituents Nigella sativa seeds, were investigated in relation to plasma, erythrocyte liver oxidative abnormalities hyperlipidemic Wistar albino rats. Pretreatment with 10 mg TQ or 200 LMN atherogenic suspension fed rats, effectively reduced plasma lipid peroxidation markers, conjugated diene, hydroperoxide, malondialdehyde, replenished capacity by increasing its ferric reducing ability...
Therapeutic role of Nigella sativa (NS) seed oil fractions, methanolic extract (ME) and volatile (VO) their constituents, thymoquinone (TQ) limonene (LMN) in relation to lipidemic-oxidative stress Wistar rats was determined. The total phenolic contents NS ME VO extracts were 320.00 ± 3.00, 300.12 0.04 288.41 0.01 mg gallic acid equivalents per 100 g oil, respectively. Their Fe+2 chelating activities 870.00 2.00, 222.31 5.80 38.59 1.43 EDTA These fractions compounds exhibited strong...
Rheumatoid arthritis (RA), a chronic inflammatory disease, and its exact aetiology is not defined clearly. The free radicals produced in large amount RA are associated with alteration molecular structure resulting glycation of proteins. As result glycation, advanced end products (AGEs) produced. In this study, collagen type II suspension was injected into Wistar rats to make model rats. Simultaneously, hesperidin 50 mg kg-1 body weight orally administrated the for 21 days. X-rays rat hind...
Nanotechnology is the study, design, creation, synthesis, manipulation, and application of functional materials, devices, delivery systems through control matter at nanometer scale. These include nanovectors, nanotubes, nanosensors, nanowires nanocantilever arrays, nanopores nanoformulations. advances will lead to significant applications relevant diagnosis, management, in treatment many diseases. The nanotechnology has attracted much interest as a new approach deliver poorly soluble drugs...
To study the structural requirements for factor IXa binding to platelets, we have carried out equilibrium studies with human after replacing second epidermal growth (EGF) domain by corresponding polypeptide region of X. The chimeric protein, IX(Xegf2), and wild-type, IXwt, produced in embryonic kidney cells 293 were radiolabelled 125I activated XIa. Direct thrombin-activated platelets showed normal stoichiometry affinity IXawt presence VIIIa (2 units/ml) X (1.5 microM). However, under...
In this study, human serum albumin (HSA), the most abundant protein of blood plasma, was modified with varying concentrations peroxynitrite. The peroxynitrite-induced changes in HSA monitored by spectroscopy, SDS-PAGE, 1-anilinonaphthalene-8-sulfonic acid (ANS), thermal denaturation studies, and matrix-assisted laser desorption/inonization-time flight mass spectrometry (MALDI-TOF MS). Aggregate formation studied thioflavin T binding scanning electron microscopy (SEM). results indicated...