A5043867797- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- HIV/AIDS Research and Interventions
- Biosensors and Analytical Detection
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Microfluidic and Bio-sensing Technologies
- Streptococcal Infections and Treatments
- Additive Manufacturing and 3D Printing Technologies
- Neonatal and Maternal Infections
- CRISPR and Genetic Engineering
- Biosimilars and Bioanalytical Methods
- Electrohydrodynamics and Fluid Dynamics
- Cytomegalovirus and herpesvirus research
- Computational Drug Discovery Methods
- Cancer, Lipids, and Metabolism
- Photochromic and Fluorescence Chemistry
- Photopolymerization techniques and applications
- Fluid Dynamics and Mixing
- Communication in Education and Healthcare
- Prostate Cancer Diagnosis and Treatment
- Infective Endocarditis Diagnosis and Management
- Prostate Cancer Treatment and Research
University of Washington
2019-2025
Seattle University
2019-2024
McGill University
2016-2022
McGill University and Génome Québec Innovation Centre
2016-2018
FX Palo Alto Laboratory
2016
Chain reactions, characterized by initiation, propagation and termination, are stochastic at microscopic scales underlie vital chemical (for example, combustion engines), nuclear biotechnological polymerase chain reaction) applications1–5. At macroscopic scales, reactions deterministic limited to applications for entertainment art such as falling dominoes Rube Goldberg machines. On the other hand, microfluidic lab-on-a-chip (also called a micro-total analysis system)6,7 was visualized an...
We recently developed capillaric circuits (CCs) – advanced capillary microfluidic devices assembled from fluidic elements in a modular manner similar to the design of electric (Safavieh & Juncker, <italic>Lab Chip</italic>, 2013, <bold>13</bold>, 4180–4189).
Urinary tract infections (UTI) are one of the most common bacterial and would greatly benefit from a rapid point-of-care diagnostic test. Although significant progress has been made in developing microfluidic systems for nucleic acid whole bacteria immunoassay tests, their practical application is limited by complex protocols, bulky peripherals, slow operation. Here we present capillaric circuit (CC) optimized automated detection urine. Molds CCs were constructed using previously established...
Microfluidics offer user-friendly liquid handling for a range of biochemical applications. 3D printing microfluidics is rapid and cost-effective compared to conventional cleanroom fabrication. Typically, are printed using digital light projection (DLP) stereolithography (SLA), but many models in use expensive (≥$10,000 USD), limiting widespread use. Recent crystal display (LCD) technology advancements have provided inexpensive (<$500 USD) SLA printers with sufficient pixel resolution...
Maintaining the efficacy of human immunodeficiency virus (HIV) medications is challenging among children because dosing difficulties, limited number approved drugs, and low rates medication adherence. Drug level feedback (DLF) can support dose optimization timely interventions to prevent treatment failure, but current tests are heavily instrumented centralized. We developed REverse transcriptase ACTivity crispR (REACTR) for rapid measurement HIV drugs based on extent DNA synthesis by reverse...
We created CandyCollect, a lollipop-inspired, child-friendly saliva sampling device that provides enhanced comfort compared to common throat and mouth swabs. The has an open microchannel functionalized surface effectively captures stores the pathogens. recently demonstrated ability combine CandyCollect with rapid antigen detection tests (RADTs) for fast accessible of group A s treptococcus (GAS). However, current procedure necessitates manual steps elute transfer captured antigens from...
Abstract Background Tenofovir diphosphate concentration in red blood cells is an objective measure of long-term oral pre-exposure prophylaxis (PrEP) or antiretroviral therapy (ART) adherence. However, current methods for measuring tenofovir are equipment and capital intensive, limiting widespread adoption. Objectives Low cost, rapid diagnostics may drive clinical adoption routine drug level measurement as a tool adherence monitoring disoproxil fumarate-based PrEP ART. We validate simple...
The CandyCollect is a lollipop-inspired open-fluidic oral sampling device designed to provide comfortable user experience. We demonstrate that the can be coupled rapid antigen detection test (RADT) kit for Group A Streptococcus (GAS). Through in vitro experiments with pooled saliva spiked pyogenes, we tested various reagents and elution volumes optimize RADT readout from samples. resulting optimized protocol uses kit-provided lateral flow assay (LFA) while replacing kit's pharyngeal swab...
Abstract Pathogens encapsulate or encode their own suite of enzymes to facilitate replication in the host. The pathogen-derived possess specialized activities that are essential for pathogen and have naturally been candidates drug targets. Phenotypic assays detecting characterizing inhibition under drugs offer an opportunity detection, resistance testing individual patients, as a research tool new development. Here, we used HIV example develop targeting reverse transcriptase (RT) enzyme...
Maintaining adequate levels of antiretroviral (ARV) medications is crucial for the efficacy HIV treatment and prevention regimens. Monitoring ARV can predict or prevent adverse health outcomes like failure drug resistance. However, conventional measurement using liquid chromatography-tandem mass spectrometry (LC-MS/MS) slow, expensive, centralized delaying clinical behavioral interventions. We previously developed a rapid enzymatic assay measuring nucleotide reverse transcriptase inhibitors...
Poor adherence to pre-exposure prophylaxis (PrEP) and antiretroviral therapy (ART) can lead human immunodeficiency virus (HIV) acquisition emergence of drug-resistant infections, respectively. Measurement antiviral drug levels provides objective information that may help prevent adverse health outcomes. Gold-standard drug-level measurement by liquid chromatography/mass spectrometry is centralized, heavily instrumented, expensive thus unsuitable unavailable for routine use in clinical...
Abstract Objective Maintaining adequate drug adherence is crucial to ensure the HIV prevention benefits of pre-exposure prophylaxis (PrEP). We developed an enzymatic assay for rapidly measuring tenofovir-diphosphate (TFV-DP) concentrations—a metabolite that indicates long-term PrEP adherence. Setting The study was conducted at Madison Clinic Harborview Medical Center in Seattle. Methods enrolled adults receiving standard oral PrEP, and individuals not any antiretrovirals. measured TFV-DP...
Abstract The CandyCollect device is a lollipop-inspired open fluidic oral sampling designed to provide comfortable user experience. We demonstrate that the can be coupled with rapid antigen detection test (RADT) kit for Group A Streptococcus (GAS). Through in vitro experiments pooled saliva spiked pyogenes we tested various reagents and elution volumes optimize RADT readout from samples. resulting optimized protocol uses kit-provided lateral flow assay (LFA) while replacing kit’s pharyngeal...
At the same time that COVID-19 cases in United States first began to increase, fellows a mentored teaching apprenticeship for postdoctoral scientists teach undergraduate seminars. The suddenly needed support students emotionally and switch online instruction.
Sufficient drug concentrations are required for efficacy of antiretroviral drugs used in HIV care and prevention. Measurement nucleotide analogs, included most medication regimens, enables monitoring short- long-term adherence the risk treatment failure. The REverSe TRanscrIptase Chain Termination (RESTRICT) assay rapidly infers concentration intracellular analogs based on inhibition DNA synthesis by reverse transcriptase enzyme. Here, we introduce a probabilistic model RESTRICT demonstrate...
Abstract Trigger valves are fundamental features in capillary-driven microfluidic systems that stop fluid at an abrupt geometric expansion and release when there is flow orthogonal channel connected to the valve. The concept was originally demonstrated closed-channel capillary circuits. We show here trigger can be successfully implemented open channels. also a series of open-channel placed alongside or opposite main resulting layered flow. developed closed form model for dynamics based on...
Engineered living materials (ELMs) are an emerging class of biohybrid that have shown great promise with advanced capabilities unachievable by conventional materials. However, application ELMs outside the laboratory has been limited due to need for periodic media replenishment or complete immersion. We herein demonstrated integration capillary microfluidics autonomous and pump-free hydration ELM hydrogels. optimized 3D printing parameters, including exposure time build plate lift retract...
Abstract Maintaining efficacy of human immunodeficiency virus (HIV) medications is challenging among children because dosing difficulties, the limited number approved drugs, and low rates medication adherence. Drug level feedback (DLF) can support dose optimization timely interventions to prevent treatment failure, but current tests are heavily instrumented centralized. We developed REverse-transcriptase ACTivity-crispR (REACTR) assay for rapid measurement HIV drugs based on extent DNA...
Ionic liquids solvate photobase generators to facilitate vat photopolymerization of thiol–ene ionogels. The base-catalyzed ionogels were more ductile than radical-mediated