A5017833546- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- T-cell and B-cell Immunology
- Social Policy and Reform Studies
- Immune Response and Inflammation
- Immunotherapy and Immune Responses
- Employment and Welfare Studies
- Retirement, Disability, and Employment
- Intergenerational Family Dynamics and Caregiving
- Cardiac Health and Mental Health
- SARS-CoV-2 and COVID-19 Research
- Autism Spectrum Disorder Research
- Irish and British Studies
- Heart Rate Variability and Autonomic Control
- Joseph Conrad and Literature
- Global Health Care Issues
- Congenital heart defects research
- Labour Market and Migration
- Lipoproteins and Cardiovascular Health
- Dendrimers and Hyperbranched Polymers
- Blood Pressure and Hypertension Studies
- Soil Carbon and Nitrogen Dynamics
- Blood transfusion and management
- Reproductive Physiology in Livestock
- interferon and immune responses
University of Central Florida
2016-2024
Yale University
2024
College of Central Florida
2016-2022
Florida College
2021
University of Limerick
2019-2020
Tallaght University Hospital
2015-2018
Smith College
2016
Ollscoil na Gaillimhe – University of Galway
2015
National University of Ireland
2012
Tissue-resident memory T cells (T
The ability to rapidly detect and diagnose acute viral infections is crucial for infectious disease control management. Serology testing the presence of virus-elicited antibodies in blood one methods used commonly clinical diagnosis infections. However, standard serology-based tests have a significant limitation: they cannot easily distinguish active from past, historical As result, it difficult determine whether patient currently infected with virus or not, on an optimal course action,...
Although clearance of many intracellular pathogens requires T-bet-dependent CD4 T cell programming, the extent to which T-bet is needed direct protective responses against influenza not known. Here, we characterize wild-type and T-bet-deficient cells during murine infection. Surprisingly, although expression has broad impacts on cytokine production by virus-specific cells, efficacy effector only marginally reduced. This reduction due lower CXCR3 expression, leading suboptimal accumulation...
Optimal transcriptional programming needed for CD4 T cells to protect against influenza A virus (IAV) is unclear. Most IAV-primed fit Th1 criteria. However, deficient the "master regulator," T-bet, although marked by reduced identity, retain robust protective capacity. In this study, we show that T-bet's paralog, Eomesodermin (Eomes), largely redundant in presence of T-bet but essential residual attributes T-bet-deficient cells. Cells lacking both and Eomes instead develop concurrent Th17...
Abstract Memory T cells can often respond against pathogens that have evaded neutralizing Abs and are thus key to vaccine-induced protection, yet the signals needed optimize their responses unclear. In this study, we identify a dramatic selective requirement for IL-6 achieve optimal memory CD4 cell recall following heterosubtypic influenza A virus (IAV) challenge of mice primed previously with wild-type or attenuated IAV strains. Through analysis endogenous adoptive transfer IAV-specific...
A well-developed, functional immune system is paramount to combat harmful attacks from pathogenic organisms and prevent infectious diseases. Newborn animals humans have only limited immunity upon birth, but their functions are expected develop within weeks months eventually reach a maturity that will provide full protection. Despite the importance of activity in animal human health management, there no convenient test available allows for rapid assessment state function nonlaboratory...
Ireland has recently experienced its worst recession in the post-World War II era.The effects on Ireland's labour market have been stark.This article examines more closely gender dimensions of recent Ireland.The evidence suggests that there gendered shifts market.With male-dominated sectors, such as construction and industry, suffering significant decline, men increasingly taking up part-time vulnerable employment, which led to a certain level re-structuring competition within these...
Specialized memory CD4 T cells that reside long-term within tissues are critical components of immunity at portals pathogen entry. In the lung, such tissue-resident (TRM) activated rapidly after infection and promote local inflammation to control levels before circulating can respond. However, optimal clearance Influenza A virus require TRM responses by other virus-specific reach lung only several days their activation in secondary lymphoid organs. Whether sentinel activity impact efficiency...
The immune health of a farm animal can have significant impact on its overall health, welfare and productivity. One the most vulnerable physiological states for both humans animals is pregnancy. Many systemic changes correlate with gravid state, including shifts in system that may ability to respond optimally pathogen challenge. Because this, it would be beneficial able monitor pregnant closely. Recently, we developed new nanoparticle-enabled rapid blood test detect ongoing responses from...
Nanoparticle-mediated cancer drug delivery remains an inefficient process. The protein corona formed on nanoparticles (NPs) controls their biological identity and, if optimized, could enhance cell uptake. In this study, a hyperbranched polyester polymer (HBPE) was synthesized from diethyl malonate and used to generate NPs that were subsequently coated with normal sera (NS) collected mice. Cellular uptake of NS-treated HBPE-NPs compared PEGylated assessed using MDA-MB-231 triple-negative...
Abstract It is generally accepted that influenza A virus (IAV) infection promotes a Th1-like CD4 T cell response and this effector program underlies its protective impact. Canonical Th1 polarization requires cytokine-mediated activation of the transcription factors STAT1 STAT4 synergize to maximize induction “master regulator” factor, T-bet. Here, we determine individual requirements for these in directing imprint primed by mice tracking virus-specific wild-type or T-bet-deficient cells...
Abstract Most CD4 T cells primed by influenza A virus (IAV) express the transcription factor T-bet, Th1 ‘master regulator’, and are marked phenotypic functional hallmarks. The extent to which STAT1 STAT4 activation, both needed for maximal T-bet induction in vitro, required direct IAV-primed cell responses is not clear. Here, we transferred STAT-deficient (STAT −/−) or wildtype recognizing IAV congenic mice, challenged them with IAV, analyzed donor infected lungs 7 days later. −/−cells were...
The aim of this study was to assess the effectiveness systematic identification and sustained follow-up protocol hypertensive patients using ambulatory blood pressure monitoring (ABPM) superimposed on Phase III cardiac rehabilitation (CR). Methods: Patients with elevated (BP) (clinic measurement) entry CR had cycles ABPM, lifestyle education, medication changes clinic BP monitoring, throughout up 6 months until control achieved. Results: Initial assessment measurement identified 30% (129)...
Optimizing the biological identity of nanoparticles (NPs) for efficient tumor uptake remains challenging. The controlled formation a protein corona on NPs through absorption from biofluids could favor that enables accumulation. To increase diversity proteins absorbed by NPs, sera derived Influenza A virus (IAV)-infected mice were used to pre-coat formed using hyperbranched polyester polymer (HBPE-NPs). HBPE-NPs, encapsulating tracking dye or cancer drug, treated with days 3-6 IAV infection...
Abstract Most CD4 T cells responding to influenza A virus (IAV) fit prototypical Th1 criteria including high expression of the transcription factor T-bet, ‘master regulator’, and strong IFNg production. Optimal development in vitro also requires cytokine-dependent activation factors STAT4 STAT1, but how these pathways impact anti-viral responses is not clear. Here, focus on roles cell-intrinsic we transferred STAT-deficient (STAT−/−) or wildtype recognizing congenic hosts, challenged with...