A5006487554- Neuroscience and Neuropharmacology Research
- Neurotransmitter Receptor Influence on Behavior
- Receptor Mechanisms and Signaling
- Nicotinic Acetylcholine Receptors Study
- Ion channel regulation and function
- Cellular transport and secretion
- Chemical synthesis and alkaloids
- Pharmacological Receptor Mechanisms and Effects
- Adenosine and Purinergic Signaling
- Neuroendocrine regulation and behavior
- Neuroscience of respiration and sleep
- Plant and fungal interactions
- Genetics and Neurodevelopmental Disorders
- Photoreceptor and optogenetics research
- Vehicle emissions and performance
- bioluminescence and chemiluminescence research
- Cardiovascular Syncope and Autonomic Disorders
- Analytical Chemistry and Chromatography
- Plant-based Medicinal Research
- Heart Rate Variability and Autonomic Control
- GABA and Rice Research
- Retinal Development and Disorders
- Circadian rhythm and melatonin
- Pain Mechanisms and Treatments
- Reproductive Biology and Fertility
Institut de Psychiatrie et Neurosciences de Paris
2004-2021
Inserm
2003-2020
Sorbonne Paris Cité
2012-2020
Université Paris Cité
2012-2020
Délégation Paris 5
2012-2020
Sorbonne Université
1992-2013
Centre National de la Recherche Scientifique
1985-2002
Pitié-Salpêtrière Hospital
1990
Laboratoire de Neurobiologie Cellulaire et Moléculaire
1990
Georgetown University
1989
Abstract: The specific binding of [ 3 H]8‐hydroxy‐2‐(di‐ n ‐propylamino)tetralin ([ H]8‐OH‐DPAT) to 5‐hydroxytryptamine (5‐HT)‐related sites was investigated in several regions the rat brain. Marked differences were observed characteristics membranes from hippocampus, striatum, and cerebral cortex. Hippocampal exhibited highest affinity ( K D > 2 M ) followed by cortex 6 striatum 10 ). Ascorbic acid inhibited H]8‐OH‐DPAT all three but millimolar concentrations Ca 2+ , Mg Mn enhanced...
Abstract Serotonin (5‐HT) is a neurotransmitter which exerts both central and peripheral control on numerous physiological functions such as sleep/wake cycle, thermoregulation, food intake, nociception, locomotion, sexual behavior, gastrointestinal motility, blood coagulation, cardiovascular homeostasis. These multiple effects are mediated by family of serotonin receptors comprises at least 13 distinct G‐protein‐coupled (GPCRs) the A type combination ligand‐gated cation channel...
Following the cloning and sequencing of A subunit 5-HT3 receptor, two alternatively spliced isoforms, 5-HT3-AS 5-HT3-AL, have been identified. In order to analyse distribution a polyclonal antibody has produced against short form which is most abundant in central nervous system [Doucet et al. (2000) Neuroscience 95, 881-892]. As expected from recognition functional receptors, immunostaining by this anti-5-HT3-R-AS matched high-affinity binding sites rat brain spinal cord. 5-HT3-AS-like...
Fast chemical communications in the nervous system are mediated by several classes of receptor channels believed to be independent functionally and physically. We show here that concurrent activation P2X2 ATP-gated 5-HT3 serotonin-gated leads functional interaction nonadditive currents (47-73% predicted sum) mammalian myenteric neurons as well Xenopus oocytes or transfected human embryonic kidney (HEK) 293 cell heterologous systems. also these two cation coimmunoprecipitate constitutively...
ATP and γ-aminobutyric acid (GABA) are two fast neurotransmitters co-released at central synapses, where they co-activate excitatory P2X inhibitory GABAA (GABA type A) receptors. We report here that co-activation of P2X2 various receptors, co-expressed in Xenopus oocytes, leads to a functional cross-inhibition dependent on subunit composition. Sequential applications GABA revealed αβ- or αβγ-containing receptors inhibited channels, whereas channels failed inhibit γ-containing This cross-talk...
The 5-HT 1A receptor (5-HT R) is the most extensively characterized serotonin (5-HT) mainly because of its involvement in mode action antidepressants. R confined to somatodendritic domain central neurons, where it mediates serotonin-evoked hyperpolarization. Our previous studies underlined role short C-terminal targeting dendrites. We used this 17 aa region as bait a yeast two-hybrid screen, and identified, for first time, an intracellular protein interacting with R. This homologous Yif1p,...
Abstract In mammals, the hormone melatonin is mainly produced by pineal gland with nocturnal peak levels. Its peripheral and central actions rely either on its intrinsic antioxidant properties or binding to MT 1 2 receptors, belonging G protein‐coupled receptor (GPCR) super‐family. Melatonin has been reported be involved in many functions of nervous system such as circadian rhythm regulation, neurotransmission, synaptic plasticity, memory, sleep, also Alzheimer's disease depression. However,...
Selective serotonin reuptake inhibitors are among the most prescribed antidepressants. Fluoxetine is lead molecule which exerts its therapeutic effects, at least in part, by promoting neuroplasticity through increased brain-derived neurotrophic factor (BDNF)/tropomyosin-related receptor kinase B (TrkB) signalling. It unclear however, to extent neuroplastic effects of fluoxetine solely mediated inhibition transporter (5-HTT). To answer this question, on were analysed both wild type (WT) and...
Abstract: In the presence of substance P (SP; 10 μ M ), serotonin (5‐HT; 1 ) triggered a cation permeability in cells hybridoma (mouse neuroblastoma X rat glioma) clone NG 108‐15 that could be assessed by measuring cell capacity to accumulate [ 14 C]guanidinium for 10‐15 min at 37°C. addition 5‐HT (EC 50 , 0.33 potent 3 receptor agonists 2‐methyl‐serotonin, phenylbiguanide, and m ‐chlorophenylbiguanide, quipazine, markedly increased uptake exposed SP. contrast, antagonists prevented effect...
Abstract: PCR was used to isolate identical partial cDNA clones encoding a serotonin 5‐HT 3 receptor subunit from rat nodose and superior cervical ganglia. The amino acid sequence predicted these clones, extending the putative transmembrane domain I stop codon, demonstrated 93% homology with A (R‐A) cloned NCB 20 hybridoma mouse neuroblastoma/Chinese hamster embryonic brain cells. Comparison of sequences gene this revealed five deletion, GSLLP, located within second intracellular loop...
Regulation of ligand-gated ion channel (LGIC) function and trafficking by cytoskeleton proteins has been the topic recent research. Here, we report that light chain (LC1) microtubule-associated protein 1B (MAP1B) specifically interacted with 5-HT(3A) receptor, a predominant serotonin-gated in brain. LC1 receptors were colocalized central neurons HEK 293 cells expressing receptors. reduced steady-state density at membrane surface significantly accelerated receptor desensitization time...
Although essential for their neuronal function, the molecular mechanisms underlying dendritic targeting of serotonin G-protein-coupled receptors are poorly understood. Here, we characterized a Yif1B-dependent vesicular scaffolding complex mediating intracellular traffic rat 5-HT 1A receptor (5-HT R) toward dendrites. By combining directed mutagenesis, GST-pull down, and surface plasmon resonance, identified tribasic motif in C-tail R on which Yif1B binds directly with high affinity ( K D ≈...