A5087640050- Neuroscience and Neuropharmacology Research
- Cellular transport and secretion
- Cannabis and Cannabinoid Research
- Receptor Mechanisms and Signaling
- Neurotransmitter Receptor Influence on Behavior
- Retinal Development and Disorders
- Sleep and Wakefulness Research
- Neurogenesis and neuroplasticity mechanisms
- Lipid Membrane Structure and Behavior
- Memory and Neural Mechanisms
- Pancreatic function and diabetes
- Neurobiology and Insect Physiology Research
- Congenital heart defects research
- Neurological disorders and treatments
- Microtubule and mitosis dynamics
- Advanced Proteomics Techniques and Applications
- Hippo pathway signaling and YAP/TAZ
- Vestibular and auditory disorders
- RNA Interference and Gene Delivery
- MicroRNA in disease regulation
- Nerve injury and regeneration
- Autophagy in Disease and Therapy
- Mass Spectrometry Techniques and Applications
- Nicotinic Acetylcholine Receptors Study
- Amino Acid Enzymes and Metabolism
Université Paris Cité
2014-2022
SPPIN - Saints-Pères Paris Institute for Neurosciences
2021-2022
Laboratoire Plasticité du Cerveau
2022
Institut de Psychiatrie et Neurosciences de Paris
2021
Délégation Paris 6
2021
Centre National de la Recherche Scientifique
2011-2018
Délégation Paris 5
2017-2018
Sorbonne Paris Cité
2017-2018
Neurophotonics Laboratory
2014-2018
Inserm
2004-2016
The CB1 cannabinoid receptor (CB1R) displays a significant level of ligand-independent (i.e. constitutive) activity, either when heterologously expressed in nonneuronal cells or neurons where CB1Rs are endogenous. present study investigates the consequences constitutive activity on intracellular trafficking CB1R. When transfected HEK-293 cells, CB1R is at plasma membrane, but substantial proportion (∼85%) receptors localized vesicles. Detailed analysis CB1-EGFP shows that population mostly...
Endocannabinoids are recently recognized regulators of brain development, but molecular effectors downstream type-1 cannabinoid receptor (CB1R)-activation remain incompletely understood. We report atypical coupling neuronal CB1Rs, after activation by endo- or exocannabinoids such as the marijuana component ∆(9)-tetrahydrocannabinol, to heterotrimeric G12/G13 proteins that triggers rapid and reversible non-muscle myosin II (NM II) dependent contraction actomyosin cytoskeleton, through a...
Numerous studies have shown that cerebellar function is related to the plasticity at synapses between parallel fibers and Purkinje cells. How specific input patterns determine outcomes, as well biophysics underlying of these synapses, remain unclear. Here, we characterize activity lead postsynaptically expressed LTP using both in vivo vitro experiments. Similar requirements LTD, find high-frequency bursts are necessary trigger this burst-dependent depends on presynaptic NMDA receptors nitric...
Hippocampal interneurons release the inhibitory transmitter GABA to regulate excitation, rhythm generation and synaptic plasticity. A subpopulation of GABAergic basket cells co-expresses GABA/glycine vesicular transporters (VIAAT) atypical type III glutamate transporter (VGLUT3); therefore, these have ability signal with both glutamate. transmission by has been extensively characterized but nothing is known about functional implications VGLUT3-dependent released cells. Here, using...
During neuronal development, neurons form elaborate dendritic arbors that receive signals from axons. Additional studies are needed to elucidate the factors regulating establishment of patterns. Our work explored possible roles played by nitric oxide synthase 1 adaptor protein (NOS1AP; also known as C-terminal PDZ ligand or CAPON) in patterning cultured hippocampal neurons. Here we report long isoform NOS1AP (NOS1AP-L) plays a novel role dendrite outgrowth and branching. NOS1AP-L decreases...
The 5-HT 1A receptor (5-HT R) is the most extensively characterized serotonin (5-HT) mainly because of its involvement in mode action antidepressants. R confined to somatodendritic domain central neurons, where it mediates serotonin-evoked hyperpolarization. Our previous studies underlined role short C-terminal targeting dendrites. We used this 17 aa region as bait a yeast two-hybrid screen, and identified, for first time, an intracellular protein interacting with R. This homologous Yif1p,...
Chronic cannabinoid exposure results in tolerance due to region-specific desensitization and down-regulation of CB1 receptors (CB1Rs). For most G-protein-coupled receptors, internalization closely follows rapid as an important component long-term down-regulation. However, vivo patterns CB1R are not known. Here we investigate the subcellular redistribution CB1Rs rat forebrain following activation by agonist CP55 940 or inhibition antagonist/inverse AM251. At steady state, mainly localized...
The 5-HT1A and 5-HT1B serotonin receptors exhibit different subcellular localizations in neurons. Evidence has been reported that the C-terminal domain is involved somato-dendritic axonal targeting of 5-HT1AR 5-HT1BR, respectively. Here we analyzed consequences mutation a di-leucine motif palmitoylated cysteines within this domain. Replacement I414-I415 by di-alanine led to endoplasmic reticulum (ER) sequestration corresponding mutant expressed cell lines as well hippocampal neurons culture....
Directionality of information flow through neuronal networks is sustained at cellular level by polarized neurons. However, specific targeting or anchoring motifs responsible for distribution on the surface have only been identified a few G-protein-coupled receptors (GPCRs). Here, mutational and pharmacological modifications conformational state two model GPCRs, axonal CB1R cannabinoid somatodendritic SSTR2 somatostatin receptors, we show important conformation-dependent variations in...
By analogy to other axonal proteins, transcytotic delivery following spontaneous endocytosis from the somatodendritic membrane is expected be essential for polarized distribution of G-protein coupled receptors (GPCRs). However, possible contribution constitutive activation, which may also result in GPCR endocytosis, poorly known. Using two closely related but differentially distributed serotonin receptors, here we demonstrate higher activation and 5-HT(1B) R, as compared 5-HT(1A) both...
Higher brain function relies on proper development of the cerebral cortex, including correct positioning neurons and dendrite morphology. Disruptions in these processes may result various neurocognitive disorders. Mutations CPE gene, which encodes carboxypeptidase E (CPE), have been linked to depression intellectual disability. However, it remains unclear whether is involved early turn contributes pathophysiology Here, we investigate effects knockdown explore functional significance...
Neuronal migration and dendritogenesis are dependent on dynamic changes to the microtubule (MT) network. Among various factors that regulate MT dynamics stability, post-translational modifications (PTMs) of MTs play a critical role in conferring specificity regulatory protein binding MTs. Thus, it is important understand regulation PTMs during brain development as multiple developmental processes In this study, we identified carboxypeptidase E (CPE) tubulin polyglutamylation, major PTM...
Memory and long term potentiation require de novo protein synthesis. A key regulator of this process is mTORC1, a complex comprising the mTOR kinase. Growth factors activate mTORC1 via pathway involving PI3-kinase, Akt, TSC GTPase Rheb. In non-neuronal cells, translocation to late endocytic compartments (LEs), where Rheb enriched, triggered by amino acids. However, regulation in neurons remains unclear. mouse hippocampal neurons, we observed that BDNF treatments activating NMDA receptors...
Membrane proteins are crucial for diverse biological processes. Optoproteomics technique enables the capture of proteome with unprecedented spatiotemporal resolution.