A5062004158- 3D Printing in Biomedical Research
- Electrospun Nanofibers in Biomedical Applications
- Renal and related cancers
- RNA Interference and Gene Delivery
- Cellular Mechanics and Interactions
- DNA and Nucleic Acid Chemistry
- Ultrasound and Hyperthermia Applications
- Renal Diseases and Glomerulopathies
- Pluripotent Stem Cells Research
- Cancer Cells and Metastasis
- Adipokines, Inflammation, and Metabolic Diseases
- Elasticity and Material Modeling
- COVID-19 and healthcare impacts
- Gastrointestinal Tumor Research and Treatment
- Chronic Kidney Disease and Diabetes
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Proteoglycans and glycosaminoglycans research
- SARS-CoV-2 and COVID-19 Research
- Angiogenesis and VEGF in Cancer
- Microfluidic and Capillary Electrophoresis Applications
- Nanofabrication and Lithography Techniques
- Bacteriophages and microbial interactions
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Diabetic Foot Ulcer Assessment and Management
University of Washington
2021-2024
University of Michigan
2011-2021
Institute for Stem Cell Biology and Regenerative Medicine
2021
Kidney Research UK
2021
Michigan Medicine
2016
Michigan United
2011
Wayne State University
1962
Sinai Hospital
1962
Human intestinal organoids (HIOs) represent a powerful system to study human development and are promising candidates for clinical translation as drug-screening tools or engineered tissue. Experimental control use of HIOs is limited by growth in expensive poorly defined tumor-cell-derived extracellular matrices, prompting investigation synthetic ECM-mimetics HIO culture. Since possess an inner epithelium outer mesenchyme, we hypothesized that adhesive cues provided the matrix may be...
Kidneys are critical target organs of COVID-19, but susceptibility and responses to infection remain poorly understood. Here, we combine SARS-CoV-2 variants with genome-edited kidney organoids clinical data investigate tropism, mechanism, therapeutics. specifically infects organoid proximal tubules among diverse cell types. Infections produce replicating virus, apoptosis, disrupted morphology, features which revealed in the context polycystic disease. Cross-validation gene expression...
Elevated interferon (IFN) signaling is associated with kidney diseases including COVID-19, HIV, and apolipoprotein-L1 (APOL1) nephropathy, but whether IFNs directly contribute to nephrotoxicity remains unclear. Using human organoids, primary endothelial cells, patient samples, we demonstrate that IFN-γ induces pyroptotic angiopathy in combination APOL1 expression. Single-cell RNA sequencing, immunoblotting, quantitative fluorescence-based assays reveal IFN-γ-mediated expression of...
Obesity-induced chronic inflammation and fibrosis in adipose tissue contributes to the progression of type 2 diabetes mellitus (DM). While is known induce mechanical stiffening numerous types, it unknown whether DM associated with alterations properties.The purpose this study was investigate differences bulk viscoelastic properties from diabetic (DM) non-diabetic (NDM) obese subjects.Bulk shear rheology performed on visceral (VAT) subcutaneous (SAT) tissue, collected subjects undergoing...
Extracellular matrix (ECM) remodeling is essential for the process of capillary morphogenesis. Here we employed synthetic poly(ethylene glycol) (PEG) hydrogels engineered with proteolytic specificity to either metalloproteinases (MMPs), plasmin, or both investigate relative contributions MMP- and plasmin-mediated ECM vessel formation in a 3D-model self-assembly analogous vasculogenesis. We first demonstrated role mechanisms an endothelial-fibroblast co-culture model vasculogenesis fibrin...
Forming functional blood vessel networks is a major clinical challenge in the fields of tissue engineering and therapeutic angiogenesis. Cell-based strategies to promote neovascularization have been widely explored, but cell sourcing remains significant limitation. Induced-pluripotent stem cell-derived endothelial cells (iPSC-ECs) are promising, potentially autologous, alternative source. However, it unclear whether iPSC-ECs form same robust microvasculature vivo documented for other EC...
Localized delivery of nucleic acids to target sites (e.g., diseased tissue) is critical for safe and efficacious gene therapy. An ultrasound‐based technique termed acoustic droplet vaporization (ADV) has been used spatiotemporally control the release therapeutic small molecules proteins contained within sonosensitive emulsions. Here, ADV lipoplex—containing plasmid DNA encoding an enhanced green fluorescent protein reporter—from a emulsion. Focused ultrasound (3.5 MHz, mechanical index (MI)...
Summary Human intestinal organoids (HIOs) represent a powerful system to study human development and are promising candidates for clinical translation as drug-screening tools or engineered tissue. Experimental control use of HIOs is limited by growth in expensive poorly defined tumor-cell-derived extracellular matrices, prompting investigation synthetic ECM-mimetics HIO culture. Since possess an inner epithelium outer mesenchyme, we hypothesized that adhesive cues provided the matrix may be...
We have used constant force axial optical tweezers to understand the subtle eects of sequence variations on mechanical properties DNA. Using designed sequences DNA with nearly identical curvatures, but varied AT content, we shown persistence length be highly dependent elasticity The varies by almost thirty percent between containing 61% and 45% AT. biological implications this can substantial, as need bend is involved in a host regulatory schemes, ranging from nucleosome positioning...
Helms, Louisa; Juliar, Benjamin A.; Himmelfarb, Jonathan; Freedman, S. Author Information