A5100668609- Respiratory viral infections research
- Reproductive Biology and Fertility
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Birth, Development, and Health
- Hepatitis B Virus Studies
- Bacteriophages and microbial interactions
- Virology and Viral Diseases
- Sarcoidosis and Beryllium Toxicity Research
- Fashion and Cultural Textiles
- Epigenetics and DNA Methylation
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Viral Infections and Vectors
- Antimicrobial Resistance in Staphylococcus
- Influenza Virus Research Studies
- Gestational Diabetes Research and Management
- Renal Diseases and Glomerulopathies
- Animal Disease Management and Epidemiology
- Sirtuins and Resveratrol in Medicine
- Bacterial Identification and Susceptibility Testing
- Plant Molecular Biology Research
- Congenital Diaphragmatic Hernia Studies
- Reproductive System and Pregnancy
- Physical Education and Training Studies
- vaccines and immunoinformatics approaches
Lishui University
2024
Academy of Military Medical Sciences
2024
First Affiliated Hospital of Chengdu Medical College
2022
Chengdu Medical College
2008-2022
Second Xiangya Hospital of Central South University
2021
Central South University
2021
Tianjin Centers for Disease Control and Prevention
2010-2020
Capital Medical University
2020
Nihon University
2020
Nanjing Agricultural University
2007-2018
Abstract Maternal obesity in humans is associated with poor outcomes across the reproductive spectrum. Emerging evidence indicates that these defects are likely attributed to factors within oocyte. Although various molecules and pathways may contribute impaired oocyte quality, prevention of fertility issues maternal a challenge. Using mice fed high‐fat diet ( HFD ) as an model, we document spindle disorganization, chromosome misalignment, elevated reactive oxygen species ROS levels oocytes...
Human respiratory adenovirus (ADV) is a highly infectious virus with potential for pandemics. There are currently no specific drugs to treat ADV worldwide, so early rapid detection of infection essential. In this study, we developed an innovative magnetic-optical triple-mode lateral flow immunoassay (LFIA) using magnetic quantum dots as immunomarkers. This novel approach addresses the need and accurate detection, allowing multimodal quantitative/semiquantitative analysis magnetic,...
Maternal diabetes has been demonstrated to adversely affect oocyte quality in mouse oocytes. However, the potential molecular mechanisms are poorly understood. Here, we established a type I diabetic model and detected increased reactive oxygen species (ROS) levels decreased Sirt3 expression oocytes from mice. Furthermore, found that forced of significantly attenuates such an excessive production ROS. The acetylation status lysine 68 superoxide dismutase (SOD2K68) is dependent on In line with...
Objective: Ferroptosis has an important role in developing pulmonary fibrosis. The present project aimed to identify and validate the potential ferroptosis-related genes fibrosis by bioinformatics analyses experiments. Methods: First, tissue sequencing data were obtained from Gene Expression Omnibus (GEO) FerrDb databases. Bioinformatics methods used analyze differentially expressed (DEGs) between normal control group extract DEGs. Hub screened enrichment analysis, protein-protein...
No licensed vaccines or therapeutic agents for human metapneumovirus (hMPV) infection exist to date. We aimed construct a multi-epitope peptide (MEP) of hMPV show promising results epitope-based vaccine development.Six independent algorithms were screened predict B-cell epitopes hMPV, and three used cytotoxic T lymphocyte helper (Th) epitopes. Predicted assembled in series with the spacers GPGPG KK introduced, termed MEP. Recombinant mep genes inserted into pET32a(+) plasmid expressed...
Aging plays a significant role in the occurrence and development of idiopathic pulmonary fibrosis (IPF). In this study, we aimed to identify verify potential aging-associated genes involved IPF using bioinformatic analysis. The mRNA expression profile dataset GSE150910 available Gene Expression Omnibus (GEO) database R software were used differentially expressed aging-related IPF. Hub gene was validated by other GEO datasets. ontology (GO) enrichment analysis Kyoto Encyclopedia Genes Genomes...
Summary The level of Sirt2 protein is reduced in oocytes from aged mice, while exogenous expression could ameliorate the maternal age‐associated meiotic defects. To date, underlying mechanism remains unclear. Here, we confirmed that specific depletion disrupts maturational progression and spindle/chromosome organization mouse oocytes, with compromised kinetochore–microtubule attachments. Candidate screening revealed acetylation state lysine 243 on BubR1 (BubR1‐K243, an integral part spindle...
Histone deacetylases (HDACs) have been shown to deacetylate numerous cellular substrates that govern a wide array of biological processes. HDAC3, member the Class I HDACs, is highly conserved and ubiquitously expressed protein. However, its roles in meiotic oocytes are not known. In present study, we find mouse depleted HDAC3 unable completely progress through meiosis, blocked at metaphase I. These knockdown show spindle/chromosome organization failure, with severely impaired...
The identification of antigenic variants and the selection influenza viruses for vaccine production are based largely on characterisation haemagglutinin (HA) circulating using haemagglutination inhibition (HI) assay. However, in addition to evolution related escape from host immunity, emerging as a result propagation different cell substrates can complicate interpretation HI results. objective was develop further micro-neutralisation (MN) assay complement assess emergence new reinforce...
SIRT7, a member of the sirtuin family, with coenzyme NAD catalyzes protein deacetylation and has been implicated in multiple biologic processes; however, its function mammalian oocytes remains to be explored. Here, we report disrupted meiotic maturation upon specific knockdown SIRT7 mouse oocytes. In particular, disorganized spindle/chromosomes loss cortical actin cap are readily observed SIRT7-depleted oocytes, generating aneuploid eggs. Furthermore, found that depletion markedly elevated...
Complicated skin and soft tissue infections (cSSTI) are some of the most commonly treated in hospitals, place heavy economic burdens on patients society. Here we report findings from an analysis cSSTI based a retrospective study which was conducted within Chinese inpatient population. We focused our research patient population, antibiotic treatment, clinical outcome burden. The population comprised 527 selected hospitalized between 2008 2013. Among hospitalizations with microbiological...
Older adults diagnosed with schizophrenia face unique public health challenges, specific treatment needs, complex care demands, accelerated aging, and increased susceptibility to issues. This phenomenological study explored the existential realities needs of older schizophrenia. Fifteen participants, a mean age 69.47 (SD ± 9.47) years, ranging from 62 79 years old hailing rural regions, participated in study. Four main themes eight sub-themes emerge: compounding burden (challenges symptom...
Sarcoidosis (SA) is an immune disorder disease featuring granulomas formation with a controversial etiopathogenesis. We aimed to uncover potential markers for SA diagnosis and explore how cell infiltration contributes the pathogenesis of SA.Sarcoidosis GSE83456 samples GSE42834 from Gene Expression Omnibus (GEO) were analyzed as training external validation sets, respectively. Firstly, R statistical software was employed differentially expressed genes (DEGs) GSE83456. Weighted gene...
Assessment of virus aerosol characteristics is important in designing methods for controlling infectious aerosols. The factors relevant to include particle size, concentration, infectivity and virulence. To investigate these their changes different environmental conditions, a laboratory model required. In this study, replication-deficient recombinant adenovirus (RDRADS) encoding green fluorescent protein (GFP) was used as virus. Model aerosols were generated using TK-3 generator attached...
Summary Persistent chronic infection with hepatitis B virus ( HBV ) is a major risk factor for the development of ‐related diseases. The molecular mechanisms that underlie and associated carcinogenesis are not fully understood. aim this study was to explore role ENO 1 in replication processes. Here, we examined expression levels ‐infected non‐ liver tissues cells by Western blot analysis, real‐time PCR immunohistochemistry. In addition, HB sAg eAg media transfected HepG2.2.15 were detected...
Intersectins (ITSNs) have been shown to act as adaptor proteins that govern multiple cellular events via regulating Cdc42 activity. However, it remains be determined whether the ITSN-Cdc42 pathway is functional in porcine oocytes. To address this question, we used a small molecule, ZCL278, selectively disrupt ITSN2-Cdc42 interaction. In present study, find oocytes exposed ZCL278 are unable completely progress through meiosis. Meanwhile, spindle defects and chromosomal congression failure...
Purpose: The factor associated with IgA nephropathy (IgAN) is an abnormality of known as galactose-deficient IgA1 (Gd-IgA1). purpose this study was to determine the molecular role played by miRNAs in formation Gd-IgA1 IgAN and investigate regulatory Astragaloside IV (AS-IV) miRNAs. Patients methods: Bioinformatics analysis, along functional mechanistic experiments, were used relationship function miRNA, β-1, 3-galactosyltransferase (C1GALT1), Gd-IgA1, AS-IV. Analyses involved a series tools,...