A5073479800- Monoclonal and Polyclonal Antibodies Research
- HER2/EGFR in Cancer Research
- Glycosylation and Glycoproteins Research
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Nanoparticle-Based Drug Delivery
- Chemical Synthesis and Analysis
- Advanced Polymer Synthesis and Characterization
- Viral gastroenteritis research and epidemiology
- Protein Degradation and Inhibitors
- Cell Adhesion Molecules Research
- Biosimilars and Bioanalytical Methods
- Advanced Proteomics Techniques and Applications
- Pesticide Residue Analysis and Safety
- Peptidase Inhibition and Analysis
- Animal Virus Infections Studies
Sanofi (United States)
2018-2022
Transitional Learning Center
2013
Antibody-drug conjugates (ADCs) have been proven clinically to be more effective anti-cancer agents than native antibodies. However, the classical conjugation chemistries prepare ADCs by targeting primary amines or hinge disulfides a number of shortcomings including heterogeneous product profiles and linkage instability. We developed novel site-specific method glycosylation site on antibodies as an approach address these limitations. The glycans Asn-297 were enzymatically remodeled in vitro...
The Multi-Attribute Method (MAM) Consortium was initially formed as a venue to harmonize best practices, share experiences, and generate innovative methodologies facilitate widespread integration of the MAM platform, which is an emerging ultra-high-performance liquid chromatography–mass spectrometry application. Successful implementation purity-indicating assay requires new peak detection (NPD) potential process- and/or product-related impurities. NPD interlaboratory study described herein...
The prevention of adventitious agent contamination is a top priority throughout the entire biopharmaceutical production process. For example, although viral cell banks or cultures rare, it can result in serious consequences (e.g., shutdown and decontamination manufacturing facilities). To ensure virus free production, numerous vivo vitro assays biophysical characterizations such as electron microscopy are conducted on banks, raw materials, process drug substances Molecular PCR other...
We report a modular approach to synthesize maleimido group containing hydrophilic dolastatin 10 (Dol10) derivatives as drug-linkers for the syntheses of antibody-drug conjugates (ADCs). Discrete polyethylene glycol (PEG) moieties different chain lengths were introduced part linker impart hydrophilicity these drug linkers. The synthesis process involved construction PEG tetrapeptide intermediate (N-methylvaline-valine-dolaisoleucine-dolaproine), which subsequently coupled with dolaphenine...
The multi-attribute method (MAM) was conceived as a single assay to potentially replace multiple single-attribute assays that have long been used in process development and quality control (QC) for protein therapeutics. MAM is rooted traditional peptide mapping methods; it leverages mass spectrometry (MS) detection confident identification quantitation of many types attributes may be targeted monitoring. While has widely explored across the industry, yet gain strong foothold within QC...
Syntheses and characterization of aminooxy terminated polymers N-(2-hydroxyproyl) methacrylamide (HPMA) controlled molecular weight narrow distribution are presented here. Design a chain transfer agent (CTA) containing N-tert-butoxycarbonyl (t-Boc) protected group enabled us to use reversible addition-fragmentation (RAFT) polymerization technique polymerize the HPMA monomer. An amide bond was utilized link CTA through triethylene glycol spacer. As result, is linked poly(HPMA) backbone...
Abstract A series of novel multivalent drug linkers (MDLs) containing cytotoxic agents were synthesized and conjugated to antibodies yield highly potent antibody–drug conjugates (ADCs) with drug/antibody ratios (DARs) higher than those typically reported in the literature (10 vs. ≈4). These MDLs contain two copies a agent attached biocompatible scaffolds composed branched peptide core discrete polyethylene glycol (PEG) chains enhance solubility decrease aggregation. produced well‐defined...
ADVERTISEMENT RETURN TO ISSUEPREVCorrectionNEXTORIGINAL ARTICLEThis notice is a correctionCorrection to Well-Defined Aminooxy Terminated N-(2-Hydroxypropyl) Methacrylamide Macromers for Site Specific Bioconjugation of GlycoproteinsPradeep K. Dhal*, Steven C. Polomoscanik, Diego A. Gianolio, Patrick G. Starremans, Michelle Busch, Kim Alving, Bo Chen, and Robert J. MillerCite this: Bioconjugate Chem. 2013, 24, 11, 1967–1968Publication Date (Web):October 30, 2013Publication History Published...