A5106730773- Advanced Proteomics Techniques and Applications
- Protein purification and stability
- Mass Spectrometry Techniques and Applications
- Echinoderm biology and ecology
- Viral Infectious Diseases and Gene Expression in Insects
- Monoclonal and Polyclonal Antibodies Research
- Calcium Carbonate Crystallization and Inhibition
- Aquaculture disease management and microbiota
- Environmental Toxicology and Ecotoxicology
- Immune Response and Inflammation
- Metabolomics and Mass Spectrometry Studies
- Trace Elements in Health
- Invertebrate Immune Response Mechanisms
- Genomics and Chromatin Dynamics
- Receptor Mechanisms and Signaling
- Marine Biology and Environmental Chemistry
- Heat shock proteins research
- Pharmacological Effects and Assays
- Physiological and biochemical adaptations
- Marine Sponges and Natural Products
- Marine Bivalve and Aquaculture Studies
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Ion-surface interactions and analysis
- Microplastics and Plastic Pollution
AstraZeneca (Australia)
2023-2024
AstraZeneca (United States)
2024
Novavax (United States)
2021-2022
Shenyang Pharmaceutical University
2018
Ningbo University
2012-2014
Michigan State University
2008-2011
The Multi-Attribute Method (MAM) Consortium was initially formed as a venue to harmonize best practices, share experiences, and generate innovative methodologies facilitate widespread integration of the MAM platform, which is an emerging ultra-high-performance liquid chromatography–mass spectrometry application. Successful implementation purity-indicating assay requires new peak detection (NPD) potential process- and/or product-related impurities. NPD interlaboratory study described herein...
Skin ulceration syndrome (SUS) is the main limitation in development of Apostichopus japonicus culture industries, which Vibrio splendidus has been well documented as one major pathogens. However, intrinsic mechanisms toward pathogen challenge and disease outbreak remain largely unknown at metabolic level. In this work, responses were investigated muscles sea cucumber among natural SUS-diseased V. splendidus-challenged samples. The did not induce obvious biological effects A. samples after...
Chemical cross-linking combined with proteolytic digestion and mass spectrometry (MS) is a promising approach to provide inter- intramolecular distance constraints for the structural characterization of protein topologies functional multiprotein complexes. Despite relative straightforwardness these methodologies, identification cross-linked proteins presents significant analytical challenge, due complexity resultant peptide mixtures, as well array inter-, intra-, or "dead-end"-cross-linked...
Host cell proteins (HCPs), which are process-related impurities typically present at low levels in recombinant biopharmaceutical products, often measured using an immunological technique, such as enzyme-linked immunosorbent assay (ELISA). In contrast to ELISA only provides the total amount of HCP, liquid chromatography/mass spectrometry (LC/MS) can provide both qualitative and quantitative information about major HCP species. this study, HCP-enrichment step was optimized combined with LC/MS...
An amine specific peptide derivatization strategy involving the use of novel isobaric stable isotope encoded 'fixed charge' sulfonium ion reagents, coupled with an analysis employing capillary HPLC, ESI-MS, and automated data dependent trap CID-MS/MS, -MS3, and/or ETD-MS/MS, has been developed for improved quantitative protein phosphorylation, identification characterization their site(s) modification. Derivatization 50 synthetic phosphopeptides S,S′-dimethylthiobutanoylhydroxysuccinimide...
Recombinant therapeutic proteins have become the major class of drugs to treat various human diseases in recent years. Low levels protein sequence variants (SVs) been reported be present recombinant proteins. The consequences potential unwanted immune response from SVs increasingly drawn attention regulatory authorities and biopharmaceutical industry. It is highly desirable detect low-level during clone selection early process development as part control strategy. Peptide mapping with...
Protein surface accessible residues play an important role in protein folding, protein-protein interactions and protein-ligand binding. However, a common problem associated with the use of selective chemical labeling methods for mapping solvent is that when complicated peptide mixture resulting from large or complex analyzed, modified peptides may be difficult to identify characterize amongst largely unmodified population (i.e., 'needle haystack' problem). To address this challenge, we...
The multi-attribute method (MAM) was conceived as a single assay to potentially replace multiple single-attribute assays that have long been used in process development and quality control (QC) for protein therapeutics. MAM is rooted traditional peptide mapping methods; it leverages mass spectrometry (MS) detection confident identification quantitation of many types attributes may be targeted monitoring. While has widely explored across the industry, yet gain strong foothold within QC...
Mass spectrometry (MS) is inherently an information-rich technique. In this era of big data, label-free MS quantification for nontargeted studies has gained increasing popularity, especially complex systems. One the cornerstones successful predictive modeling ionization efficiency (IE) based on solutes' physicochemical properties. While many have studied IE small molecules, there are limited reports peptide IEs. study, we leverage stoichiometric relationship in trypsin digests...
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Ku is a heterodimer composed of p70 and p80, the regulatory subunit DNA-dependent protein kinase. As multifunctional DNA-binding complex, plays important roles in DNA damage repair through non-homologous end joining V(D)J recombination. In addition, has also been implicated various biological functions including growth control, cell proliferation, cycle, chromosome maintenance, transcriptional regulation, apoptosis, viral infection. particular, using our Inverse Genomics (Immusol, Inc., San...