A5046705745- Receptor Mechanisms and Signaling
- Pharmacological Effects and Assays
- Cardiac electrophysiology and arrhythmias
- Neuropeptides and Animal Physiology
- Asthma and respiratory diseases
- Neuroscience and Neuropharmacology Research
- Cardiac Ischemia and Reperfusion
- Heart Failure Treatment and Management
- Neurotransmitter Receptor Influence on Behavior
- Inhalation and Respiratory Drug Delivery
- Signaling Pathways in Disease
- Nitric Oxide and Endothelin Effects
- Ion channel regulation and function
- Protein Kinase Regulation and GTPase Signaling
- Analytical Chemistry and Chromatography
- Pharmacological Receptor Mechanisms and Effects
- Renin-Angiotensin System Studies
- Phosphodiesterase function and regulation
- Andrographolide Research and Applications
- Cardiovascular Function and Risk Factors
- Hippo pathway signaling and YAP/TAZ
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Medicinal Plants and Neuroprotection
- Moringa oleifera research and applications
- Nuts composition and effects
Cornell University
2023
Shenyang Pharmaceutical University
2016-2022
Peking University
2011-2020
Center for Life Sciences
2014-2018
The University of Tokyo
2017
National Institutes of Health
2007-2014
Chinese University of Hong Kong
2001-2011
National Institute on Aging
2007-2011
Institute on Aging
2007-2010
SRI International
2007-2010
Ca(2+)-calmodulin-dependent protein kinase II (CaMKII) is expressed in many mammalian cells, with the delta isoform predominantly cardiomyocytes. Previous studies have shown that inhibition of CaMKII protects cardiomyocytes against beta(1)-adrenergic receptor-mediated apoptosis. However, it unclear whether activation sufficient to cause cardiomyocyte apoptosis and signaling important heart muscle cell mediated by other stimuli. Here, we specifically enhanced or suppressed activity using...
Recent studies revealed that the herb <i>Andrographis paniculata</i> possesses cardioprotective activities. Using neonatal rat cardiomyocytes, actions of several diterpene lactones derived from <i>A. including andrographolide, 14-deoxyandrographolide, 14-deoxy-11,12-didehydroandrographolide, and sodium 14-deoxyandrographolide-12-sulfonate were investigated. Pretreatment with andrographolide but not other compounds protected cardiomyocytes against hypoxia/ reoxygenation injury up-regulated...
Baicalin and its aglycone baicalein are the major flavonoid components of root Scutellaria baicalensis. Recent studies have shown that they can attenuate oxidative stress in various vitro models as possess potent antioxidant activities. This study investigated alternative protective mechanisms a cardiomyocyte model.Neonatal rat cardiomyocytes pretreated with test compound were subjected to hypoxia/reoxygenation. The extent cellular damage was accessed by amount released lactate...
A fundamental question regarding receptor-G protein interaction is whether different agonists can lead a receptor to intracellular signaling pathways. Our previous studies have demonstrated that although most β<sub>2</sub>-adrenoceptor activate both G<sub>s</sub> and G<sub>i</sub> proteins, fenoterol, full agonist of β<sub>2</sub>-adrenoceptor, selectively activates protein. Fenoterol contains two chiral centers may exist as four stereoisomers. We synthesized series stereoisomers fenoterol...
We have reported therapeutic effectiveness of pharmacological stimulation β<sub>2</sub> adrenoreceptors (ARs) to attenuate the cardiac remodeling and myocardial infarction (MI) expansion in a rat model dilated cardiomyopathy (DCM) post-MI. Furthermore, combination AR with β<sub>1</sub> blockade exceeded blockade. However, these studies were relatively short (6 weeks). In this study, same experimental model, we compared different effects, including survival benefit, combined therapy blocker,...
Stereoisomers of fenoterol and six derivatives have been synthesized their binding affinities for the β2 adrenergic receptor (Kiβ2-AR), subtype selectivity relative to β1-AR (Kiβ1-AR/Kiβ2-AR) functional activities were determined. Of 26 compounds in study, submicromolar observed (R,R)-fenoterol, (R,R)-isomer p-methoxy, (R,R)- (R,S)-isomers 1-naphthyl all these active at concentrations cardiomyocyte contractility tests. The Kiβ1-AR/Kiβ2-AR ratios >40 (R,R)-fenoterol (R,R)-p-methoxy...
Objective: There is increasing interest in the study of antioxidant actions plant phenolic compounds as evidence shows that consumption products rich these contributes to protection from a number ailments including cardiovascular diseases. In present study, effects selected dietary sources, namely barbaloin, 6-gingerol and rhapontin, were investigated.Methods: Low-density lipoprotein (LDL), erythrocytes erythrocyte membranes subjected several vitro oxidative systems. The assessed by their...
Interaction of a given G protein-coupled receptor to multiple different proteins is widespread phenomenon. For instance, β2-adrenoceptor (β2-AR) couples dually Gs and Gi proteins. Previous studies have shown that cAMP-dependent protein kinase (PKA)-mediated phosphorylation β2-AR causes switch in coupling from Gi. More recent demonstrated by kinases, particularly GRK2, markedly enhances the coupling. We previously although most agonists cause both activation, (R,R′)-fenoterol preferentially...
Rationale: The β 2 -adrenoceptor (β -AR), a prototypical GPCR (G protein-coupled receptor), couples to both G s and i proteins. Stimulation of the -AR is beneficial humans animals with heart failure presumably because it activates downstream -PI3K-Akt cell survival pathway. Cardiac signaling can be regulated by crosstalk or heterodimerization other GPCRs, but physiological pathophysiological significance this type regulation has not been sufficiently demonstrated. Objective: Here, we aim...
Astrocytomas and glioblastomas have been particularly difficult to treat refractory chemotherapy. However, significant evidence has presented that demonstrates a decrease in astrocytoma cell proliferation subsequent an increase cAMP levels. The 1321N1 line, as well other astrocytomas glioblastomas, expresses β(2)-adrenergic receptors (β(2)-ARs) are coupled G(s) activation consequent production. Experiments were conducted determine whether the β(2)-AR agonist (R,R')-fenoterol agonists could...
We sought to investigate the association of single nucleotide polymorphisms (SNPs) genes involved in βAR signaling with response patients blockers. A total 2403 hospitalized chronic heart failure (HF) were enrolled a multicenter observational study as first cohort and followed up for mean period 20 months. Genes β1AR, β2AR, major cardiac G-protein-coupled receptor kinases (GRKs) GRK2 GRK5 analyzed identify SNPs, stratified according genotypes. second independent enrolling 919 HF was applied...
Psoralen and isopsoralen, furocoumarins isolated from the plant Psoralea corylifolia L., were demonstrated to exhibit in vitro inhibitory actions on monoamine oxidase (MAO) activities rat brain mitochondria, preferentially inhibiting MAO-A activity over MAO-B activity. This inhibition of enzyme was found be dose-dependent reversible. For MAO-A, IC50 values are 15.2 +/- 1.3 microM psoralen 9.0 0.6 isopsoralen. MAO-B, 61.8 4.3 12.8 0.5 Lineweaver-Burk transformation data indicates that by both...
Abstract During cardiac muscle development, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcyclin‐binding protein/Siah‐interacting protein (CacyBP/SIP) as one of candidates being upregulated in hyperplastic to hypertrophic switch, suggesting an important role CacyBP/SIP development. To show importance during myoblast differentiation, report here that is developmentally regulated postnatal rat hearts. The...
Stimulation of β1-adrenergic receptor (β1AR), a GPCR, and the for advanced glycation end-products (RAGE), pattern recognition (PRR), have been independently implicated in pathogenesis cardiomyopathy caused by various etiologies, including myocardial infarction, ischemia/reperfusion injury, metabolic stress. Here, we show that two distinctly different receptors, β1AR RAGE, are mutually dependent mediating injury sequelae cardiomyopathy. Deficiency or inhibition RAGE blocks β1AR- RAGE-mediated...
Cardiac fibrosis is a pathological feature commonly found in hearts exposed to haemodynamic orneurohormonal stress. Elevated levels of arginine vasopressin (AVP) are closely associated with the progression heart failure and could be an underlying cause cardiac fibrosis. The aim this study characterize effect AVP on neonatal rat fibroblasts (NRCFs) illustrate its signalling mechanism. proliferative was assessed by methylthiazolyldiphenyl-tetrazolium assay 5-bromo-2'-deoxyuridine (BrdU)...