A5075826396- Liver Disease Diagnosis and Treatment
- Metabolism and Genetic Disorders
- Genomics and Phylogenetic Studies
- Liver Disease and Transplantation
- Evolution and Genetic Dynamics
- Genetic diversity and population structure
- Organ Transplantation Techniques and Outcomes
- Chromosomal and Genetic Variations
- Metabolomics and Mass Spectrometry Studies
- RNA and protein synthesis mechanisms
- Clinical Nutrition and Gastroenterology
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- CRISPR and Genetic Engineering
- Drug-Induced Hepatotoxicity and Protection
- Bat Biology and Ecology Studies
- Muscle metabolism and nutrition
- Biochemical effects in animals
- Viral Infections and Vectors
- Diet and metabolism studies
- Genetic and Kidney Cyst Diseases
- Electrolyte and hormonal disorders
- Microbial Community Ecology and Physiology
- Genetics, Aging, and Longevity in Model Organisms
- Mitochondrial Function and Pathology
Université Claude Bernard Lyon 1
2012-2025
Centre National de la Recherche Scientifique
2016-2025
Inserm
1994-2025
École Normale Supérieure de Lyon
2016-2025
Centre International de Recherche en Infectiologie
2024-2025
Institut des Sciences de l'Evolution de Montpellier
2020
Laboratoire de Biologie et Modélisation de la Cellule
2018-2019
Laboratoire de Biométrie et Biologie Evolutive
2016-2019
Fédération de recherche en imagerie multi-modalité
2017
Sorbonne Université
1990-2015
Actinopterygian fishes harbor at least eight distinct pigment cell types, leading to a fascinating diversity of colors. Among this diversity, the cellular origin white color appears be linked several types such as iridophores or leucophores. We used clownfish Amphiprion ocellaris, which has pattern consisting bars over darker body, characterize cells that underlie hue. observe by electron microscopy in are similar iridophores. In addition, transcriptomic signature exhibits similarities with...
In the history of life, some phenotypes have been acquired several times independently, through convergent evolution. Recently, lots genome-scale studies devoted to identify nucleotides or amino acids that changed in a manner when evolved. These efforts had mixed results, probably because differences detection methods, and conceptual about definition substitution. Some methods contend substitutions are only if they occur on all branches where phenotype toward exact same state at given...
Bats are asymptomatic reservoirs of several zoonotic viruses. This may result from long-term coevolution between viruses and bats, that have led to host adaptations contributing an effective balance strong antiviral responses with innate immune tolerance. To better understand these virus-host interactions, we combined comparative transcriptomics, phylogenomics functional assays characterize the evolution bat factors. First, stimulated type I interferon pathway in Myotis yumanensis primary...
Some species undergo programmed DNA elimination (PDE), whereby portions of the genome are systematically destroyed in somatic cells. PDE has emerged independently several phyla, but its function is unknown. Although mechanisms partially solved ciliates, remains mysterious metazoans because study were not yet amenable to functional approaches. We fortuitously discovered massive free-living nematode genus Mesorhabditis, from same family as C. elegans. As such, these offer many experimental...
Ribosomal proteins (r-proteins) are increasingly used as an alternative to ribosomal rRNA for prokaryotic systematics. However, their routine use is difficult because r-proteins often not or wrongly annotated in complete genome sequences, and there currently no dedicated exhaustive database of r-proteins. RiboDB aims at fulfilling this gap. This weekly updated comprehensive allows the fast easy retrieval r-protein sequences from publicly available sequences. The current version contains 90...
Abstract The genus Myotis is one of the largest clades bats, and exhibits some most extreme variation in lifespans among mammals alongside unique adaptations to viral tolerance immune defense. To study evolution longevity-associated traits infectious disease, we generated near-complete genome assemblies cell lines for 8 closely related species . Using genome-wide screens positive selection, analyses structural variation, functional experiments primary lines, identify new patterns adaptation...
Understanding the genetic underpinnings of fitness trade-offs across spatially variable environments remains a major challenge in evolutionary biology. In Mediterranean gilthead sea bream, first-year juveniles use various marine and brackish lagoon nursery habitats characterized by trade-off between food availability environmental disturbance. Phenotypic differences among foraging different rapidly appear after larval settlement, but relative role local selection plasticity phenotypic...
We evaluated the rat cirrhosis model obtained by repeated intraperitoneal administration of CCl4 (group C) with regard to biological and nutritional conditions in comparison ad libitum AL) pair-fed control rats. Cirrhotic rats were divided into two groups according their clinical condition: group C1 (n = 4) represented those good physical condition C2 10) poor condition. Autopsy indicated that suffered from severe malnutrition as judged body weight, carcass weight carcass/body ratio....
The effects of norepinephrine on portal blood flow hepatic extraction and intrinsic clearance taurocholate were studied in the isolated perfused rat liver. Livers at a constant pressure. [14C]Taurocholate was infused continuously rate 0.6 mumol/min. Then, three increasing doses added to perfusate. Norepinephrine induced significant dose-dependent reduction flow. In spite this reduction, did not increase decreased significantly. When mechanically, was, as expected, flow-independent....
To determine the hepatic fate of alpha-ketoisocaproate (KIC) in cirrhosis, six groups isolated rat livers were perfused with 0, 0.5, 1 (with or without alpha-[1-14C]KIC), 2, and 5 mM KIC; control from healthy rats studied parallel under similar conditions. KIC was rapidly removed by normal livers, whereas uptake lower cirrhotic at all concentrations tested (at 2 mM, 4.04 +/- 0.33 vs. 6.32 0.58 mumol/min; P < = 0.05). The transamination pathway, evaluated leucine exchanges, more important...
We report the first case in medical literature (to our knowledge) of a patient with human immunodeficiency virus infection who was being treated ritonavir and developed signs severe vascular involvement irreversible coma after administration 3 mg ergotamine tartrate.
RNA sequencing (RNA-Seq) is a widely used approach to obtain transcript sequences in non-model organisms, notably for performing comparative analyses. However, current bioinformatic pipelines do not take full advantage of pre-existing reference data related species improving RNA-Seq assembly, annotation and gene family reconstruction.We built an automated pipeline named CAARS combine novel from experiments with existing multi-species alignments. reads are assembled into transcripts by both...
Abstract All genomes contain repeated sequences that are known as transposable elements (TEs). Among these endogenous retroviruses (ERVs), which similar to and transmitted across generations from parent progeny. These controlled in through epigenetic mechanisms. At the center of control TEs small interfering RNAs piRNA class, trigger heterochromatinization TE sequences. The tirant ERV Drosophila simulans displays intra-specific variability copy numbers, insertion sites, transcription levels,...
Abstract In most species, elaborate programs exist to protect chromatin and maintain its integrity over cell cycles generations. However some species systematically undergo excision elimination of portions their genome in somatic cells a process called programmed-DNA (PDE). PDE involves the mainly repeated elements but also protein-coding genes. has been described approximately 100 from very distinct phyla, more extensively parasitic nematodes Ascaris unicellular Ciliates. Ciliates, where is...
ABSTRACT Repeated adaptations rely in part on convergent genetic changes. The extent of changes at the genomic scale is debated and may depend interplay between different factors. Rodents have repeatedly adapted to life arid conditions, notably with altered renal morphology physiology. This occurred time periods, allowing us test importance evolution. We analyzed kidney transcriptomes from 34 species quantify characterize evolution level gene expression, tissue composition, coding sequences....
Abstract In the history of life, some phenotypes have been acquired several times independently, through convergent evolution. Recently, lots genome-scale studies devoted to identify nucleotides or amino acids that changed in a manner when evolved. These efforts had mixed results, probably because differences detection methods, and conceptual about definition substitution. Some methods contend substitutions are only if they occur on all branches where phenotype towards exact same state at...
We examined the possible contribution of liver to alterations in branched-chain amino acid (BCAA) metabolism cirrhosis. The livers male Sprague-Dawley rats with CCl4-induced cirrhosis were removed and placed a recirculating perfusion system. Net uptake release determined over 55 min. Results compared those obtained control animals, which either pair-fed or fed ad libitum. Intrahepatic concentrations at end perfusion. isoleucine leucine was significantly lower cirrhotic than controls There no...