A5108197624- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Enterobacteriaceae and Cronobacter Research
- Antibiotic Use and Resistance
- Bacterial Identification and Susceptibility Testing
- Escherichia coli research studies
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Influenza Virus Research Studies
- Vibrio bacteria research studies
- Plant Stress Responses and Tolerance
- Respiratory viral infections research
- Infections and bacterial resistance
- Mycobacterium research and diagnosis
- Polysaccharides and Plant Cell Walls
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Plant Pathogenic Bacteria Studies
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Antimicrobial Resistance in Staphylococcus
Yangzhou University
2012-2025
Songjiang District Central Hospital
2025
Shanghai Jiao Tong University
2025
Xinjiang Academy of Agricultural and Reclamation Science
2023-2024
East China University of Science and Technology
2024
Shanxi Medical University
2022-2023
Second Hospital of Shanxi Medical University
2022-2023
Zhongnan Hospital of Wuhan University
2023
Wuhan University
2023
Hua Medicine (China)
2023
The objective of this study was to assess the impact diverse plasmids bearing colistin resistance gene mcr-1 on host fitness. Forty-seven commensal E. coli isolates recovered from pig farm where first identified were screened for mcr-1. mcr-1-bearing characterized by sequencing. fitness evaluated in vitro competition assays. Twenty-seven (57.5%) positive genes mainly located belonging IncI2 (n = 5), IncX4 11), IncHI2/ST3 8), IncFII 2), and IncY 2). InHI2 also carried other (floR, blaCTX-M,...
The purpose of this study was to examine the occurrence fosfomycin-resistant Escherichia coli from chickens and characterize plasmids carrying fosA3. A total 661 E. isolates chicken origin collected 2009 2011 were screened for plasmid-mediated fosfomycin resistance determinants by PCR. Plasmids characterized using PCR-based replicon typing, plasmid multilocus sequence restriction fragment length polymorphisms. Associated addiction systems genes identified PCR-mapping used analysis genetic...
Introduction Salinity critically affects the growth and development of alfalfa (Medicago sativa), making it necessary to understand molecular mechanism alfalfa’s adaptation salt stress. Methods In this study, roots were subjected stress transcriptomics metabolomics analyses performed. Results The results showed that flavonoid synthesis, hormone transduction pathways may be involved in reaction, they are related. Combined analysis differential genes metabolites found dihydroquercetin...
Salmonellosis, caused by non-typhoidal Salmonella , is a common foodborne gastrointestinal infection. Third-generation cephalosporins are recommended as the first-line treatment for infections. Our study aimed to investigate molecular epidemiology, antimicrobial resistance, and transmission of extended-spectrum β-lactamases (ESBL) genes in 96 clinical isolates collected between 2020 2022 at tertiary hospital Shenzhen, China. We performed susceptibility testing whole-genome sequencing...
It is widely accepted that antibiotics provide a critical selective pressure for the horizontal transfer of antibiotic resistance between bacterial species. This study demonstrated combination low doses kanamycin and streptomycin, which inhibited growth recipient donor cells, respectively, had positive effects on transmission conjugation plasmids pRK2013, pSU2007, RP4 from Escherichia coli DH5α to HB101 at their minimum inhibitory concentrations (MICs). Administration either alone as well...
To understand the underlying evolution process of F33:A-:B- plasmids among Enterobacteriaceae isolates various origins in China, complete sequences 17 blaCTX-M-harboring obtained from Escherichia coli and Klebsiella pneumoniae different sources (animals, animal-derived food, human clinics) China were determined. shared similar plasmid backbones comprising replication, leading, conjugative transfer regions differed by numbers repeats yddA traD presence group II intron, except that pHNAH9...
ABSTRACT Background Hepatocellular carcinoma (HCC) and colorectal cancer (CRC) pose a significant threat to human health worldwide, characterized by intricate pathogenesis. A PC‐esterase domain containing 1A ( PCED1A ) is critical number of the GDSL/SGNH superfamily. Aim The aim this study explore diagnostic value in HCC CRC its relationship with immune infiltration. Methods Cancer Genome Atlas (TCGA) database, Gene Expression Omnibus (GEO) Cell Line Encyclopedia database (CCLE), Human...
A novel influenza virus, subtype H7N9, circulated through China in 2013–2014. Its higher rates of human infection a wide range locations within and the associated increased likelihood human-to-human transmission have caused global concern. Recombinant subunit vaccines provide safe targeted protection against viral infections. However, protective efficacy recombinant tends to be less potent than made from whole viruses. Studies shown that bacterial flagellin has strong adjuvant activity...
Bisphenol A (BPA) is one of the world's most widely used plasticizer, and its hazardous impacts have been well studied. However, few studies focused on effects parental long-term BPA exposure bone development offspring. In present study, offspring was studied following zebrafish to environmentally relevant 15 225 µg/L BPA. The results showed that increased mortality deformity rate caused craniofacial deformities characterized by changes in various cartilage angles lengths. alizarin red...
Carbapenem-resistant Klebsiella pneumoniae (CRKP) infection is a worldwide health issue that poses serious threat to public health. This study summarizes the clinical features of four patients with CRKP coproducing NDM and KPC infections further analyses molecular typing, resistance virulence factors strains.Of twenty-two isolates, strains coharbouring blaKPC blaNDM isolated from were screened by Sanger sequencing between October 2019 April 2021. Demographics, pathological data collected...
The purpose of this study was to investigate the occurrence plasmid-mediated colistin resistance gene mcr-1 in Enterobacteriaceae isolates from companion animals Guangzhou, China. isolated 180 samples collected cats and dogs were screened for by PCR sequencing. MCR-1-producing further characterized multilocus sequence typing pulsed-field gel electrophoresis (PFGE). Plasmid characterization performed conjugation, replicon typing, S1-PFGE, Southern blot hybridization. pHN6DS2 as a...
ABSTRACT Salmonella enterica serovar Kentucky ( S. Kentucky) has been regarded as a common serotype causing human nontyphoidal salmonellosis, frequently associated with the consumption of contaminated poultry products. Recently, multidrug-resistant (MDR) S . ST198 strong resistance to cefotaxime, ciprofloxacin, and tigecycline emerged detected in both humans Europe Asia. In this study, whole-genome sequencing (WGS) analysis divided 327 isolates into two clades, which ST198.2 is more...
Objective: Salmonella enterica serovar Kentucky ST198 has emerged as a global threat to humans. In this study, we aimed characterize the prolonged carriage of ciprofloxacin-resistant and extended-spectrum β-lactamase (ESBL)-producing S . in single patient with inflammatory bowel disease (IBD). Methods: Three strains were collected from IBD on 11th January, 23rd 8th February, 2022, respectively. Antimicrobial susceptibility testing, whole-genome sequencing, phylogenetic analysis 38 previously...
Abstract The emergence and quick spread of the plasmid-mediated tigecycline resistance gene tet (X4) colistin mcr-1 have posed a great threat to public health raised global concerns. It is imperative develop rapid accurate detection systems for onsite surveillance (X4). In this study, we developed one-tube recombinase polymerase amplification (RPA) CRISPR-Cas12b integrated systems. We identified mcr-1- (X4)-conserved -specific protospacers through comprehensive BLAST search based on NCBI nt...
The rise of the plasmid-encoded colistin resistance gene <i>mcr-1</i> is a major concern globally. Here, during routine surveillance, an unexpectedly high prevalence <i>Escherichia coli</i> with reduced susceptibility to (69.9%) was observed in Chinese broiler farm. Fifty-three (63.9%)<i> E. isolates were positive for <i>mcr-1</i>. All identified <i>mcr-1</i>-positive <i>E. (MCREC) multidrug resistant and carried other clinically significant genes. Furthermore, genes mainly located on IncI2...
This study aimed to investigate the prevalence and characterization of tet(X4) in Escherichia coli isolates from a pig farm Shanghai, China, elucidate dissemination mechanism this swine farm. Forty-nine (80.33%) E. strains were isolated 61 samples screened for presence tet(X). Among them, six (12.24%) positive exhibited resistance tigecycline (MIC ≥ 16 mg/L). They further sequenced by Illumina Hiseq. Six tet(X4)-positive belonged ST761 with identical genes, profiles, plasmid replicons,...