A5080064542- MicroRNA in disease regulation
- Cancer-related Molecular Pathways
- Cancer Cells and Metastasis
- Sesquiterpenes and Asteraceae Studies
- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- Cell death mechanisms and regulation
- Ocular Oncology and Treatments
- Sarcoma Diagnosis and Treatment
- RNA modifications and cancer
- Ovarian cancer diagnosis and treatment
- Bioactive Compounds and Antitumor Agents
- Pluripotent Stem Cells Research
- Ubiquitin and proteasome pathways
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Cervical Cancer and HPV Research
- Cancer Research and Treatments
- Endometrial and Cervical Cancer Treatments
- Carcinogens and Genotoxicity Assessment
- Genomics, phytochemicals, and oxidative stress
- Renal and related cancers
- Gestational Trophoblastic Disease Studies
- Cancer, Hypoxia, and Metabolism
- bioluminescence and chemiluminescence research
University of Malta
2020-2024
Temple University
2018-2024
Azienda Ospedaliero Universitaria Maggiore della Carita
2024
Università degli Studi del Piemonte Orientale “Amedeo Avogadro”
2024
University of Palermo
2009-2019
Clinica Luganese Moncucco
2015
Tecnologie Avanzate (Italy)
2015
Laboratoire de Biochimie
2013
Terra
2005
Triple-negative breast cancers (TNBCs) are clinically aggressive forms associated with a poor prognosis. We evaluated the cytotoxic effect exerted on triple-negative MDA-MB231 cancer cells both by parthenolide and its soluble analogue dimethylamino (DMAPT) explored underlying molecular mechanism. The drugs induced dose- time-dependent decrement in cell viability, which was not prevented caspase inhibitor z-VAD-fmk. In particular first hours of treatment (1–3 h), DMAPT strongly stimulated...
Abstract Triple-negative breast cancers (TNBCs) are aggressive forms of carcinoma associated with a high rate recidivism. In this paper, we report the production mammospheres from three lines TNBC cells and demonstrate that both parthenolide (PN) its soluble analog dimethylaminoparthenolide (DMAPT) suppressed induced cytotoxic effects in cancer stem-like cells, derived dissociation mammospheres. particular, drugs exerted remarkable inhibitory effect on viability cells. Such an was by N...
Abstract A novel cancer stem‐like cell line (3AB‐OS), expressing a number of pluripotent stem markers, was irreversibly selected from human osteosarcoma MG‐63 cells by long‐term treatment (100 days) with 3‐aminobenzamide (3AB). 3AB‐OS are heterogeneous and stable population composed three types fibroblastoid cells, spindle‐shaped, polygonal‐shaped, rounded‐shaped. With respect to extremely smaller, possess much greater capacity form spheres, stronger self‐renewal ability higher levels cycle...
// Rosa Drago-Ferrante 1 , Francesca Pentimalli 2 Daniela Carlisi 3 Anna De Blasio Christian Saliba 4 Shawn Baldacchino 5 James Degaetano 6 Joseph Debono 7 Gordon Caruana-Dingli Godfrey Grech Scerri 6, 8 Giovanni Tesoriere 9, 11 Antonio Giordano 10 Renza Vento 1, Riccardo Di Fiore Laboratory of Biochemistry, Department Biological, Chemical and Pharmaceutical Sciences Technologies, University Palermo, Polyclinic, Italy Oncology Research Center Mercogliano (CROM), Istituto Nazionale per lo...
Triple-negative breast cancer is a group of aggressive cancers with poor prognosis owing to chemoresistance, recurrence and metastasis. New strategies are required that could reduce chemoresistance increases the effectiveness chemotherapy. The results presented in this paper, showing parthenolide (PN) prevents drug resistance MDA-MB231 cells, represent contribution one these possible strategies. most studied line TNBC were submitted selection treatment mitoxantrone (Mitox) doxorubicin (DOX)....
The sesquiterpene lactone Parthenolide (PN) exerted a cytotoxic effect on MDA‐MB231 cells, triple‐negative breast cancer (TNBC) cell line, but its effectiveness was scarce when employed at low doses. This represents an obstacle for therapeutic utilization of PN. In order to overcome this difficulty we associated PN the suberoylanilide hydroxamic acid (SAHA), histone deacetylase inhibitor. Our results show that SAHA synergistically sensitized cells It is noteworthy treatment with alone...
Osteosarcoma (OS) is the most common type of bone cancer, with a peak incidence in early childhood. Emerging evidence suggests that treatments targeting cancer stem cells (CSCs) within tumor can halt and improve patient survival. MicroRNAs (miRNAs) have been implicated maintenance CSC phenotype, thus, identification CSC-related miRNAs would provide information for better understanding CSCs. Downregulation miRNA-29 family members (miR-29a/b/c; miR‑29s) was observed human OS, however, little...
Abstract Finding new treatments targeting cancer stem cells (CSCs) within a tumor seems to be critical halt and improve patient survival. Osteosarcoma is an aggressive affecting adolescents, for which there no second‐line chemotherapy. Uncovering molecular mechanisms underlying the development of osteosarcoma origin CSCs crucial identify possible therapeutic strategies. Here, we aimed characterize genetically molecularly human 3AB‐OS CSC line, previously selected from MG63 proved have both...
Osteosarcoma (OS), an aggressive highly invasive and metastatic bone-malignancy, shows therapy resistance recurrence, two features that likely depend on cancer stem cells (CSCs), which hold both self-renewing malignant potential. So, effective anticancer therapies against OS should specifically target destroy CSCs. We previously found the let-7d microRNA was downregulated in 3AB-OS-CSCs, derived from human OS-MG63 cells. Here, we aimed to assess whether modulation affected tumorigenic...
Abstract The present study shows that nuclear factor erythroid 2‐related 2 (NRF2) and miR‐29b‐1‐5p are two opposite forces which could regulate the fate of MDA‐MB‐231 cells, most studied triple‐negative breast cancer (TNBC) cell line. We show NRF2 activation stimulates growth markedly reduces reactive oxygen species (ROS) generation, whereas overexpression increases ROS generation proliferation. Moreover, downregulates expression, decreases p‐AKT p‐NRF2. Furthermore, induces both inhibition...
Abstract The mechanism of the cytotoxic effect exerted by parthenolide on tumor cells is not clearly defined today. This article shows that stimulates in human osteosarcoma MG63 and melanoma SK‐MEL‐28 a cell death, which prevented z‐VAD‐fmk other caspase inhibitors. In particular treatment with rapidly stimulated (1–2 h) reactive oxygen species (ROS) generation inducing activation extracellular signal‐regulated kinase 1/2 (ERK 1/2) NADPH oxidase. event caused depletion thiol groups...
The synthetic cannabinoid WIN 55,212-2 is a potent receptor agonist with anticancer potential.Experiments were performed to determine the effects of on proliferation, cell cycle distribution, and programmed death in human osteosarcoma MG63 Saos-2 cells.Results show that induced G2/M arrest, which was associated induction main markers ER stress (GRP78, CHOP TRB3).In treated cells we also observed conversion cytosolic form autophagosome marker LC3-I into LC3-II (the lipidated located membrane)...
More than 50% of all gynecologic tumors can be classified as rare (defined an incidence ≤6 per 100,000 women) and usually have a poor prognosis owing to delayed diagnosis treatment. In contrast almost other common solid tumors, the treatment (RGT) is often based on expert opinion, retrospective studies, or extrapolation from tumor sites with similar histology, leading difficulty in developing guidelines for clinical practice. Currently, cancer research, due distinct scientific technological...
Abstract Osteosarcoma is the second leading cause of cancer‐related death for children and young adults. In this study, we have subcutaneously injected—with without matrigel—athymic mice (Fox1nu/nu) with human osteosarcoma 3AB‐OS pluripotent cancer stem cells (CSCs), which previously isolated from MG63 cells. Engrafted were highly tumorigenic matrigel greatly accelerated both tumor engraftment growth rate. CSC xenografts lacked crucial regulators beta‐catenin levels (E‐cadherin, APC,...
Abstract Paclitaxel (PTX) and beta‐lapachone (LPC) are naturally occurring compounds that have shown a large spectrum of anticancer activity. In this article we show for the first time PTX/LPC combination induces potent synergistic apoptotic effects in human retinoblastoma Y79 cells. Combination suboptimal doses PTX (0.3 nM) LPC (1.5 µM) caused biochemical morphological signs apoptosis at 48 h treatment. These were accompanied by lowering inhibitor proteins activation Bid caspases 3 6 with...
Triple-negative breast cancer (TNBC) is a clinically aggressive form of that unresponsive to endocrine agents or trastuzumab. TNBC accounts for ~10-20% all cases and represents the with poorest prognosis. Patients are at higher risk early recurrence, mainly in lungs, brain soft tissue, therefore, there an urgent need new therapies. The present study was carried out MDA-MB-231 cells, where we assessed role caspase-8 (casp-8), critical effector death receptors, also involved non‑apoptotic...