A5022222695- Chromosomal and Genetic Variations
- Plant Genetic and Mutation Studies
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- DNA Repair Mechanisms
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Microtubule and mitosis dynamics
- Wheat and Barley Genetics and Pathology
- CRISPR and Genetic Engineering
- Plant Molecular Biology Research
- Bioenergy crop production and management
- Fungal and yeast genetics research
- Biotin and Related Studies
- Ubiquitin and proteasome pathways
- Advanced Proteomics Techniques and Applications
- Plant Reproductive Biology
- Mitochondrial Function and Pathology
Leibniz Institute of Plant Genetics and Crop Plant Research
2019-2025
University of Birmingham
2014-2017
Universidad Complutense de Madrid
2007-2011
Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, induction via mutation of centromere-specific histone H3 (cenH3) has been shown when mutant outcrossed wild-type, and wild-type genome remains progeny. However, factors that affect are still poorly understood. Here, we report cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as inducer pollinated by wild-type. We discovered short-term temperature stress knl2...
Summary During meiosis, the formation of crossovers ( CO s) generates genetic variation and provides physical links that are essential for accurate chromosome segregation. s occur in context a proteinaceous axis. The transcriptomes proteomes anthers meiocytes comprise several thousand genes proteins, but because level complexity relatively few have been functionally characterized. Our understanding functional interactions between meiotic proteins is also limited. Here we use affinity...
Abstract Meiosis generates genetic variation through homologous recombination (HR) that is harnessed during breeding. HR occurs in the context of meiotic chromosome axes and synaptonemal complex. To study role axis remodelling crossover (CO) formation a crop species, we characterized mutants axis-associated protein ASY1 axis-remodelling PCH2 Brassica rapa. asy1 plants form fail to synapse. CO almost abolished, residual chiasmata are proportionally enriched terminal regions, particularly...
<title>Abstract</title> Double haploid technology is a critical tool for accelerating plant breeding. The manipulation of centromere-specific histone H3 (CENH3) has previously been shown to induce progeny in both the model Arabidopsis and certain crop species. However, universally applicable strategy CENH3-based induction across diverse species still lacking. Here, we demonstrate that extreme asymmetry centromeric CENH3 levels between parental chromosomes causal factor eliminating with...
Meiotic crossovers (COs) shape genetic diversity by mixing homologous chromosomes at each generation. CO distribution is a highly regulated process. assurance forces the occurrence of least one obligatory per chromosome pair, homeostasis smoothes out number COs when faced with variation in precursor and interference keeps multiple away from other along chromosome. In several organisms, it has been shown that cytoskeleton are transduced to meiotic nucleus via KASH- SUN-domain proteins,...
Abstract Wild plants can contribute valuable genes to their domesticated relatives. Fertility barriers and a lack of genomic resources have hindered the effective use crop-wild introgressions. Decades research into barley’s closest wild relative, Hordeum bulbosum , grass native Mediterranean basin Western Asia, yet manifest themselves in release cultivar bearing alien genes. Here, we construct pangenome bulbous barley comprising ten haplotype-resolved genome sequence assemblies....
Abstract Key message In barley ( Hordeum vulgare ), MTOPVIB is critical for meiotic DSB and accompanied SC CO formation while dispensable bipolar spindle formation. Homologous recombination during meiosis assures genetic variation in offspring. Programmed DNA double-strand breaks (DSBs) are repaired as crossover (CO) or non-crossover (NCO) recombination. The topoisomerase VI (TopoVI) B subunit (MTOPVIB) plays an essential role CO-recombination. More recently has been also shown to play a...
We used rye-specific repetitive DNA sequences in fluorescence situ hybridization (FISH) to paint the rye genome and identify a wheat background. A 592 bp fragment from dispersed family R173 (named UCM600) was cloned as FISH probe. UCM600 is over seven chromosomes, being absent pericentromeric subtelomeric regions. similar pattern of distribution also observed on B but with weaker signals. The patterns using probe were comparable those obtained genomic (GISH) procedure. There were, however,...
<title>Abstract</title> The proteinaceous synaptonemal complex (SC) structure forms between meiotic homologous chromosomes. Its central region (CR) consists of transverse filament and element proteins, in Arabidopsis<italic> </italic>ZYP1 SCEP1/SCEP2, respectively. We describe a novel CR protein Arabidopsis. SCEP3 spatiotemporally overlaps with other components is conserved plants. In <italic>scep3</italic>, SC formation, crossover (CO) assurance (minimum one CO per chromosome pair),...
The suppression of meiotic loss when the maize B chromosomes are unpaired is genetically determined. Two genotypes were selected in 1B x 0B crosses: H line where transmission rate Mendelian (50%) and L present only about 40% progeny. Using ZmBs probe located at centromere distal portion chromosome FISH, we found that centromeric telomeric ends univalent co-orient metaphase I. This feature seems to promote proper orientation causing lack B. co-orientation was observed both lines, however...
An interstitial constriction located on the long arm of rye chromosome 5R (5RL) shows neocentromeric activity at meiosis. In some meiocytes this region is strongly stretched orienting with true centromere to opposite poles metaphase I, and keeping sister chromatid cohesion anaphase I. We found previously that frequency neocentric varied dramatically in different generations suggesting effect environmental factors. Here we studied behavior 5RL neocentromere mono- ditelosomic 5RL, mono-,...
Abstract Double haploid production is the most effective way of creating true-breeding lines in a single generation. In Arabidopsis , induction via mutation centromere-specific histone H3 (cenH3) has been shown when outcrossed to wild-type. Here we report that mutant cenH3 assembly factor KNL2 can be used as inducer. We elucidated short temperature stress knl2 increased efficiency from 1 10%. Moreover, have demonstrated point CENPC-k motif sufficient generate inducing lines, suggesting crops...