A5051615888- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Genomics and Chromatin Dynamics
Leibniz Institute of Plant Genetics and Crop Plant Research
2021-2024
Double haploid production is the most effective way to create true-breeding lines in a single generation. In Arabidopsis, induction via mutation of centromere-specific histone H3 (cenH3) has been shown when mutant outcrossed wild-type, and wild-type genome remains progeny. However, factors that affect are still poorly understood. Here, we report cenH3 assembly factor Kinetochore Null2 (KNL2) can be used as inducer pollinated by wild-type. We discovered short-term temperature stress knl2...
KINETOCHORE NULL2 (KNL2) plays key role in the recognition of centromeres and new CENH3 deposition. To gain insight into origin diversification KNL2 gene, we reconstructed its evolutionary history plant kingdom. Our results indicate that gene plants underwent three independent ancient duplications ferns, grasses eudicots. Additionally, demonstrated previously unclassified genes could be divided two clades αKNL2 βKNL2 eudicots γKNL2 δKNL2 grasses, respectively. KNL2s all encode conserved...
Abstract In eukaryotic organisms, proper chromosome segregation during cell division depends on the centromeric histone H3 (CENH3) variant. Our previous studies identified a plant CENH3 assembly factor, Kinetochore Null2 (αKNL2), that possesses centromere-targeting motif, CENPC-k, similar to CENPC motif in CENP-C. Additionally, we have demonstrated αKNL2 can bind DNA vitro, independent of its CENPC-k motif. Thus, mechanism underlying binding remains elusive. study shows and motifs alone are...
Abstract In eukaryotes, accurate chromosome segregation during cell division relies on the centromeric histone H3 variant, CENH3. Our previous work identified KINETOCHORE NULL2 (αKNL2) as a plant CENH3 assembly factor, which contains centromere-targeting motif, CENPC-k, analogous to CENPC motif found in CENP-C. We also demonstrated that αKNL2 can bind DNA vitro sequence-independent manner, without involvement of its CENPC-k motif. this study, we show and motifs alone are insufficient for...
Abstract Double haploid production is the most effective way of creating true-breeding lines in a single generation. In Arabidopsis , induction via mutation centromere-specific histone H3 (cenH3) has been shown when outcrossed to wild-type. Here we report that mutant cenH3 assembly factor KNL2 can be used as inducer. We elucidated short temperature stress knl2 increased efficiency from 1 10%. Moreover, have demonstrated point CENPC-k motif sufficient generate inducing lines, suggesting crops...