A5000426439- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Marine and coastal ecosystems
- Microbial Community Ecology and Physiology
- Telomeres, Telomerase, and Senescence
- Aquatic Ecosystems and Phytoplankton Dynamics
- Prostate Cancer Treatment and Research
- Cytomegalovirus and herpesvirus research
- Protist diversity and phylogeny
- Diagnosis and Treatment of Venous Diseases
- Immune responses and vaccinations
- Electromagnetic Fields and Biological Effects
- Signaling Pathways in Disease
- Heat shock proteins research
- Cardiac Fibrosis and Remodeling
- Cancer Immunotherapy and Biomarkers
- Lymphatic System and Diseases
- Spaceflight effects on biology
- RNA Research and Splicing
- CAR-T cell therapy research
- Peripheral Artery Disease Management
- Neuroinflammation and Neurodegeneration Mechanisms
- Coronary Interventions and Diagnostics
- RNA modifications and cancer
Hamad bin Khalifa University
2018-2020
Qatar Foundation
2019-2020
Austrian Academy of Sciences
1998-2008
Ludwig Boltzmann Institute for Age Research
2001-2006
Universität Innsbruck
1999-2004
Innsbruck Medical University
2004
University Hospital of Zurich
1990
Summary In this study we analysed the effects of age on T and B lymphocytes in human lymph nodes by comparing lymphocyte subsets paraffin sections from node tissue taken healthy young elderly people. We demonstrate that relative number CD8 + cells decreases with but CD4 does not. There is also a very pronounced age‐dependent loss CD45RA naïve cells. The size follicles CD20 are similar donors. For polymerase chain reaction analysis T‐cell receptor (TCR) repertoire TCR‐γ gene rearrangements...
Smooth muscle cell (SMC) accumulation is a key event in the development of atherosclerosis, including vein bypass graft arteriosclerosis. Because members protein kinase C (PKC) family signal cells to undergo proliferation, differentiation, or apoptosis, we generated PKCδ knockout mice and performed grafts on these animals. PKCδ–/– developed normally were fertile. Vein segments from isografted carotid arteries recipient either genotype led more severe arteriosclerosis than was seen with...
Limitation of lifespan in replicative senescence is related to oxidative stress, which probably both the cause and consequence impaired mitochondrial respiratory function. The respiration senescent human diploid fibroblasts was analysed by highresolution respirometry. To rule out cell-cycle effects, proliferating growth-arrested young were used as controls. Uncoupled respiration, normalized citrate synthase activity, remained unchanged, reflecting a constant capacity chain....
Smooth muscle cell (SMC) accumulation is a key event in the development of atherosclerosis, including vein bypass graft arteriosclerosis. Because members protein kinase C (PKC) family signal cells to undergo proliferation, differentiation, or apoptosis, we generated PKCδ knockout mice and performed grafts on these animals. PKCδ–/– developed normally were fertile. Vein segments from isografted carotid arteries recipient either genotype led more severe arteriosclerosis than was seen with...
The highly conserved and ubiquitous heat shock proteins (HSP) are essential for the cellular homeostasis efficiently trigger responses to stress conditions. Both microbial human HSP act as dominant antigens in numerous infectious autoimmune diseases such atherosclerosis, inducing a strong immune-inflammatory response. In present study, surface localization of HSP60 on stressed unstressed umbilical venous endothelial cells (HUVECs) was investigated using sensitive high resolution microscopy...
Abstract: One of the most striking changes in primary lymphoid organs during human aging is progressive involution thymus. As a consequence, rate naïve T cell output dramatically declines with age and peripheral pool shrinks. These lead to increased incidence severe infections decreased protective effect vaccinations elderly. Little is, however, known composition function residual repertoire elderly persons. To evaluate impact on pool, we investigated quantity, phenotype, function,...
The World Health Organization (WHO) predicts that by 2020 tobacco will become the largest single health problem worldwide and cause an estimated 8.4 million deaths annually (http://www5.who.int/tobacco/). Although impact of smoking on human is well defined from medical point view, surprisingly little known about mechanisms which smoke mediates its disastrous effects. Here, we demonstrate dramatically changes vascular endothelial cell tissue morphology, leading to a loss barrier function...
Abstract We have recently described an IL-2/IL-4-producing CD8+CD25+ nonregulatory memory T cell population that occurs in a subgroup of healthy elderly persons who characteristically still good humoral response after vaccination. The present study addresses this specific subset and investigates its origin, clonal composition, Ag specificity, replicative history. demonstrate cells frequently exhibit CD4+CD8+ double-positive phenotype. expression the CD8 αβ molecule occurrence signal-joint...
Based on the combined expression of CD27 and CD28, a putative model T cell differentiation has been previously proposed. We used CD28 in order to comparatively study size, cytokine production capacity proliferative response CD4+ sub-populations from healthy young elderly volunteers. Elderly persons had lower percentage CD27+CD28+ but higher CD27−CD28+ CD27−CD28−CD4+ cells than persons. were present, although at relatively low numbers, vast majority donors only sporadically detected Each...
ABSTRACT High‐risk human papillomaviruses (HPVs) are major etiological agents of cervical cancer. Despite excellent epidemiological evidence for a direct role HPV‐16 in carcinogenesis, molecular pathways underlying carcinogenesis vivo remain obscure. The E7 gene is required immortalization and maintenance the transformed phenotype vitro; however, little known about its tumorigenesis vivo. codes an unstable protein abundance which biopsies unknown. We show here that levels strongly increase...
Abstract Already at initial phases of infection, HIV is coated with complement fragments. During the chronic phase, when HIV-specific IgGs appear, virus circulates immune complexed IgG and complement. Thus, we studied interaction dendritic cells (DCs) DC-T cell cocultures (C)-opsonized C-IgG-opsonized HIV. infection monocyte-derived DCs circulating BDCA-1-positive was significantly reduced upon presence virus-specific but non-neutralizing IgGs. exposed to C-Ig-HIV or IgG-opsonized showed an...
Flow cytometry and fluorescence-activated cell sorting have become invaluable tools to analyze isolate specific populations in a wide range of biomedical research clinical applications. In countless approaches worldwide, scientists are using single analyses better understand the significance variation within different cellular populations, has major technique for isolation both basic research. However, majority available sorters pressurized, droplet-based systems, which apply significant...
AME Aquatic Microbial Ecology Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 18:95-103 (1999) - doi:10.3354/ame018095 Comparison of a direct live count and an improved quantitative protargol stain (QPS) in determining abundance cell volumes pelagic freshwater protozoa Gerald Pfister1,2,*, Bettina Sonntag2, Thomas Posch2 1Institute Limnology, Austrian Academy Sciences, Gaisberg 116, A-5310...
Drug Prescribing for Patients with Chronic Kidney Disease in General Practice: a Cross-Sectional Study
Abstract BACKGROUND Dispersed prostatic neuroendocrine cells are involved in growth regulation of the prostate and considered to play a role pathogenesis carcinoma benign hyperplasia (BPH). They meant either be derived from neural crest during embryogenesis or by direct differentiation locally present precursor cells. METHODS An vitro model was developed for human epithelial cell differentiation. Minced explants radical prostatectomies were seeded on collagen I–coated plates. RESULTS The...
Abstract Programmed cell death 1 (PD-1) is critical for T regulatory cells (Tregs) to maintain peripheral tolerance self-antigens. In the tumor microenvironment, interaction between PD-1 and its ligands supports immune evasion. Pembrolizumab blocks interactions of with ligands, enhancing antitumor clinical responses. We others have reported that pembrolizumab does not affect function or phenotype thymic-derived Tregs; however, little known about effect on extrathymic differentiation Tregs....
The effects of experimentally reduced total phosphorus concentrations (TP) from 2.4 to 0.6 µmol L −1 on plankton community in a shallow lake were evaluated the proportion between dissolved reactive P (DRP), nonreactive (DOP) and particulate organic (POP), stoichiometry among carbon (POC), nitrogen (PON), POP. In both triple ratios, DRP:DOP:POP POC:PON:POP, we used POP as key component indicate shifts fractions nutrients matter. enhanced accumulation by planktonic assemblage 14% at supply was...