A5100407153- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- CRISPR and Genetic Engineering
- Bone Metabolism and Diseases
- Viral Infections and Immunology Research
- Marine Sponges and Natural Products
- Microtubule and mitosis dynamics
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- TGF-β signaling in diseases
- Prostate Cancer Treatment and Research
- Bone health and treatments
- Molecular Biology Techniques and Applications
Inner Mongolia Medical University
2018-2023
University of Science and Technology of China
2021
Second Military Medical University
2019-2020
Changhai Hospital
2019-2020
Liaocheng University
2019
Abstract Background Aberrant alternative splicing plays critical role in aging and age-related diseases. Heterogeneous nuclear ribonucleoproteins (hnRNPs) reportedly regulate RNA process. Whether how hnRNPs contribute to neurodegenerative diseases, especially Alzheimer’s disease (AD), remain elusive. Methods Immunoblotting immunostaining were performed determine expression patterns cellular/subcellular localization of the long isoform hnRNP D-like (L-DL), which is a family member, mouse...
Study Design. An in vitro biological study. Objective. The aim of this study was to explore the role bone morphogenetic protein-2 (BMP-2) regulation osteoclast-mediated osteolysis, and possible mechanism involving BMP-2 nuclear factor-kappa B (NF-κB) signaling pathways. Summary Background Data. Recombinant human (rhBMP-2) has been approved as a therapeutic agent spinal fusion defect repair. However, its efficacy clinical application are limited by associated complications including...
The post‐transcriptional events regulating expression of the adipogenesis associated Mth938 domain containing (AAMDC) protein are poorly understood. Here, we find that AAMDC expresses three isoforms due to alternative polyadenylation (APA) and splicing (AS). Luciferase assay revealed two with short long 3′UTRs is differentially controlled. Further analyses showed isoform displays higher translation efficiency miR‐2428/664a reduces isoform, indicating APA‐mediated shortening 3′UTR renders...
Ghrelin (GHRL) is a polypeptide that can specifically bind to the growth hormone secretagogue receptor (GHSR). The expression of GHSR significantly different in normal and prostate cancer (PC) tissues humans. It important find an effective diagnostic method for diagnosis prognosis invasive PC/neuroendocrine (NEPC).GHRL mRNA levels were determined by quantitative real-time polymerase chain reaction PC tissues. GHRL proteins was assessed Western blot assay immunohistochemistry. A probe...
Introns, as important vectors of biological functions, can influence many stages mRNA metabolism. However, in recent research, post-spliced introns are rarely considered. In this study, the optimal matched regions between and their mRNAs nine model organism genomes were investigated with improved Smith-Waterman local alignment software. Our results showed that distributions frequencies highly consistent or universal. There frequency peaks UTR regions, which obvious, especially 3'-UTR. The...
<p>Supplementary Figure 3, Carmil3 KO in tumor cells reduces metastasis. Related to Fig. 3. A. Analysis of migration with scratch assay. B. Result transwell assay measur-ing cell migratory capacity. Cells which migrated down through the membrane lower chamber plate were determined MTS C. WT or 4T1 tu-mor injected subcutaneously mammary fat pad BALB/c mice. The mice sacrificed at day 28 and lung metastasis was by 6-thioguanine Representative images metastatic colonies culture shown....
<p>Supplementary Figure 3, Carmil3 KO in tumor cells reduces metastasis. Related to Fig. 3. A. Analysis of migration with scratch assay. B. Result transwell assay measur-ing cell migratory capacity. Cells which migrated down through the membrane lower chamber plate were determined MTS C. WT or 4T1 tu-mor injected subcutaneously mammary fat pad BALB/c mice. The mice sacrificed at day 28 and lung metastasis was by 6-thioguanine Representative images metastatic colonies culture shown....