This study aims to investigate the effects of probiotics and Chinese medicine polysaccharides (CMPs) on growth performance, blood indices, rumen fermentation, bacteria composition in lambs. Forty female lambs were randomly divided into four groups as follows: control, probiotics, CMP, compound (probiotics + CMP) groups. The results showed that treatment increased concentrations glucose (GLU) immunoglobulin G (IgG) enhanced microbial protein contents but declined value pH fluid compared with...

Introduction Niacin is one of the essential vitamins for mammals. It plays important roles in maintaining rumen microecological homeostasis. Our previous study indicated that dietary niacin significantly elevated intramuscular fat content (IMF) castrated finishing steers. Whether affects deposition by regulating microbial composition and functional capacities gastrointestinal microbiome has been unknown yet. Methods In this study, 16 Xiangzhong Black cattle were randomly assigned into either...

The aim of this study was to investigate the effects storage temperature and preservation time on microbial diversity community composition rumen fluid. Rumen fluid samples were collected from six Hu sheep fed a high-forage diet stored at −80 °C −20 for intervals 0, 7, 14, 30, 60, 120, 240 days. DNA extracted each point 16S rRNA gene sequencing evaluate composition. results showed that affected only relative abundance Proteobacteria, with no substantial impact alpha-diversity or other groups...

A rapid lateral flow immunoassay screening method has been developed for the determination of sulfamonomethoxine (SMM) residues in swine urine. For this purpose, a specific monoclonal antibody (mAb), SMM4B9 SMM, was generated and characterized. The mAb showed low cross-reactivity (not larger than 0.3%) to other sulfonamides potentially occurring analytes. Based on competitive principle, strip with applied SMM residues. test is made up sample pad, gold-conjugate reagent blotted membrane...

The present aim was to investigate the effects of traditional Chinese medicine prescriptions (TCM) on body temperature, blood physiological parameters, nutrient apparent digestibility and growth performance beef cattle under heat stress conditions. Twenty-seven were randomly divided into three groups as following; i) high temperature control (HTC), ii) I+high (TCM I) iii) II+high II) (n = 9 per group). results showed that mean declined in TCM II treatment (p<0.05). Serum T3 T4 levels with I...

Minimizing mortality losses due to multiple stress and obtaining maximum performance are the production goals for newly received cattle. In recent years, vaccination metaphylaxis treatment significantly decreased rate of cattle, while growth block induced by is still obvious. Assessment blood metabolites behavior monitoring offer potential early identification morbid animals. Moreover, ruminal microorganisms' homeostasis a guarantee beef steers' health. The most critical period cattle...

The present study investigates the theoretical basis for maintaining normal physiological functions in heat-stressed beef cattle by exploring effects of niacin supplementation on permeability rumen epithelial cell barrier. Herein, 12 Jinjiang bulls with an average weight approximately 400 ± 20.0 kg were randomly divided into three groups, thermoneutral (TN), (HS), and niacin-supplemented (HN) 4 each group. experiment spanned 70 days, plasma concentrations D-lactic acid, diamine oxidase...

To understand the effects of niacin on ruminal microbial ecology cattle under high-concentrate diet condition, Illumina MiSeq sequencing technology was used. Three with rumen cannula were used in a 3 × Latin-square design trial. diets fed to these during periods for days, respectively: high-forage (HF; forage-to-concentrate ratio = 80:20), (HC; 20:80), and HC supplemented 800 mg/kg (HCN). Ruminal pH measured before feeding every 2 h after initiating feeding. fluid sampled at end each period...

This study was conducted to evaluate the dietary use of vegetable oil sources in grass carp (Ctenopharyngodon idella). Fish were fed diets having same lipid level from fish (FO), palm (PO), rapeseed (RO), soybean (SO) or linseed (LO). The results showed that oils significantly decreased feed utilization and a lower growth observed SO group, while no effect on performance. Dietary suppressed intestinal digestion by inhibiting activities digestive enzymes. Vegetable elevated lipase, hepatic...

The experiment was conducted to investigate the effects of dietary supplementation with Puerarin on meat quality, muscle antioxidant ability, and fiber characteristics beef cattle under a hot environment in summer. Thirty-two 15 ± 1.5-month-old Jinjiang bulls (291.65 8.84 kg) were randomly divided into four groups at 0 (control), 200 (Pue200), 400 (Pue400), 800 (Pue800) mg/kg feed concentrate ( n = 8). feeding trial lasted for 60 days after 10-day adaptation period (July 1–September 8);...

An experiment was conducted to determine the effects of supplementing diet Jinjiang bulls with guanidinoacetic acid (GAA) on their feed efficiency [feed were evaluated feedlot average daily gain (ADG), intake (ADFI), and feed-to-gain ratio (F:G)], blood measures, meat quality. Forty-five (24 ± 3 months old 350.15 30.39 kg by weight) randomly distributed among five experimental groups (each n = 9) each group fed one diets (concentrate: roughage 60:40): (1) control; (2) 0.05% GAA; (3) 0.1% (4)...

Agricultural straws, such as rice straw, wheat and corn are produced abundantly every year but not utilized efficiently in China. An experiment was conducted to determine the effects of recombinant xylanase on ruminal fermentation microbial community structure vitro incubation these straws. The from Lentinula edodes (rLeXyn11A) Pichia pastoris. optimal temperature pH for rLeXyn11A were 40°C 4.0, respectively. featured resistance high showed broad adaptability (>50% maximum activity at...

Rape straw cannot be efficiently degraded and utilized by ruminants due to its severe lignification complex cross-linked structure between fiber lignin. The laccases can catalyze the inter-unit bond cleavage in lignin substrates. Therefore, this study investigated recombinant laccase from Lentinula edodes (LeLac) application degrading rape straw. LeLac was expressed using Pichia pastoris. It had maximum activity at 60 °C pH 3.0 ABTS as substrate 50 4.0 o-tolidine substrate. exhibited...

The aim of this study was to compare the differences dietary tea leaves (TL) and residue (TR) inclusion on rumen fermentation characteristics explore whether TR could be an alternative feedstuff ruminants. For these purposes, seven treatments consisted two types (TL vs. TR) three levels (g/g dry matter basis) 10% (TL10/TR10), 20% (TL20/TR20), 30% (TL30/TR30) in each type, plus control group with 0% (CON) were designed, four replicates group, conduct vitro ruminal test. Results showed that...

This study evaluated the effect of glutamate supplementation on physiological parameters heat-stressed Hu sheep. Forty-eight male sheep with an average initial body weight 17.74 ± 0.17 kg were randomly divided into two groups: The control group (CON) was fed a basal diet and treatment (GLU) + 3 g/head/day L-glutamate. There six replications in each four replication for 90 days feeding test. Growth performance, serum biochemistry, hormones measured during phase 1 (1-30 days), 2 (31-60 (61-90...

The use of rape straw in ruminant production is limited by its high lignin content and low ruminal degradability.White rot fungi are the most efficient known degraders lignin.Four white were investigated for their potential to degrade improve rumen fermentation straw.Solid state was carried out 0 30 days determine changes chemical composition vitro fermentation.Results showed that Phanerochaete chrysosporium Lentinula edodes degraded about 45% enhanced organic matter digestibility (IVOMD)...

This study aims to evaluate the effect of puerarin on performance, meat quality, and serum indexes beef cattle under hot environment. Thirty-two bulls were divided into four groups fed diet supplemented with at 0, 200, 400, or 800 mg/kg. Results showed that heat stress was employed for 54 out 60 days, 400 mg/kg group declined cortisol (COR) contents, all treatments increased contents total cholesterol, high density lipoprotein superoxide dismutase activity; in addition, glutathione...