A novel inertial focusing platform creates a single-stream microparticle train in single-focal plane without sheath fluids and external forces, all high-throughput manner. The proposed design consists of low-aspect-ratio straight channel interspersed with series constrictions height arranged orthogonally, making use geometry-induced secondary flows. Focusing efficiency as high 99.77% is demonstrated throughput 36 000 particles s−1 for variety different sized cells.

We present an inertial platform for high-throughput, high-efficiency and sheathless particle blood cell separation in straight channels with microstructures.

Abstract Complex three-dimensional (3D)-shaped particles could play unique roles in biotechnology, structural mechanics and self-assembly. Current methods of fabricating 3D-shaped such as 3D printing, injection moulding or photolithography are limited because low-resolution, low-throughput complicated/expensive procedures. Here, we present a novel method called optofluidic fabrication for the generation complex polymer based on two coupled processes: inertial flow shaping ultraviolet (UV)...

Cell therapy and cellular engineering begin with internalizing synthetic biomolecules functional nanomaterials into primary cells. Conventionally, electroporation, lipofection, or viral transduction has been used; however, these are limited by their cytotoxicity, low scalability, cost, and/or preparation complexity, especially in Thus, a universal intracellular delivery method that outperforms the existing methods must be established. Here, we present versatile platform leverages intrinsic...

Abstract The intrinsic biophysical states of neutrophils are associated with immune dysfunctions in diseases. While advanced image‐based flow cytometers can probe cell deformability at high throughput, it is nontrivial to couple different sensing modalities (e.g., electrical) measure other critical attributes including viability and membrane integrity. Herein, an “optics‐free” impedance‐deformability cytometer for multiparametric single mechanophenotyping reported. microfluidic platform...

This review outlines Chimeric antigen receptor (CAR)-T cell manufacturing, highlights challenges, and explores successful microfluidic approaches related technologies to address them.

Here we present a novel microfluidic technique for on-chip surface enhanced Raman spectroscopy (SERS) based biomolecular detection, exploiting the use of electrokinetically active microwells. Briefly, chip comprises series channels containing embedded microwells that, when electrically actuated, either locally attract or repulse species from solution through combination electrokinetic effects. We demonstrate that approach combines advantages existing homogeneous (solution phase) and...

Fluid inertia has been used to position microparticles in confined channels because it leads precise and predictable particle migration across streamlines a high-throughput manner. To focus particles, typically two inertial effects have employed: of particles combination with geometry-induced secondary flows. Still, the strong scaling fluid velocity or channel flow rate made challenging design focusing systems for single-stream using large-scale microchannels. Use microchannels (≥100 μm)...

Surface enhanced Raman scattering (SERS) is an analytical sensing method that provides label-free detection, molecularly specific information, and extremely high sensitivity. The enhancement makes this attractive mainly attributed to the local amplification of incident electromagnetic field occurs when a surface plasmon mode excited at metallic nanostructure. Here, we present simple, cost effective for creating flexible, large area SERS-active substrates using new technique call shadow mask...

The introduction of nanomaterials into cells is an indispensable process for studies ranging from basic biology to clinical applications. To deliver foreign living cells, traditionally endocytosis, viral and lipid nanocarriers or electroporation are mainly employed; however, they critically suffer toxicity, inconsistent delivery, low throughput time-consuming labor-intensive processes. Here, we present a novel inertial microfluidic cell hydroporator capable delivering wide range various...

Mechanical biomarkers associated with cytoskeletal structures have been reported as powerful label-free cell state identifiers. In order to measure mechanical properties, traditional biophysical (e.g., atomic force microscopy, micropipette aspiration, optical stretchers) and microfluidic approaches were mainly employed; however, they critically suffer from low-throughput, low-sensitivity, and/or time-consuming labor-intensive processes, not allowing techniques be practically used for biology...

A pulsed laser activated cell sorter (PLACS) integrated with 3D sheathless inertial focusing sorts cells high purity at high-throughput using a single layer PDMS channel that can be easily fabricated low cost. Sort of >90% is achieved throughput 10 000 particles s−1 without severe dilution effects from volume sheath flows. As service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed may re-organized for online...

The successful intracellular delivery of exogenous macromolecules is crucial for a variety applications ranging from basic biology to the clinic. However, traditional methods such as those relying on viral/non-viral nanocarriers or physical membrane disruptions suffer low throughput, toxicity, and inconsistent performance are time-consuming and/or labor-intensive. In this study, we developed single-step hydrodynamic cell deformation-induced platform named "hydroporator" without aid vectors...

In recent nanobiotechnology developments, a wide variety of functional nanomaterials and engineered biomolecules have been created, these numerous applications in cell biology. For to fulfill their promises completely, they must be able reach biological targets at the subcellular level with high specificity. Traditionally, either nanocarrier- or membrane disruption-based method has used deliver inside cells; however, methods are suboptimal due toxicity, inconsistent delivery, low throughput,...

Whole-cell-based therapy has been extensively used as an effective disease treatment approach, and it rapidly changed the therapeutic paradigm. To fully accommodate this shift, advances in genome modification cell reprogramming methodologies are critical. Traditionally, molecular tools such viral polymer nanocarriers electroporation have norm for internalizing external biomolecules into cells cellular engineering. However, these approaches not satisfactory considering their cytotoxicity,...

We present a novel nonlinear microfluidic cell stretching (μ-cell stretcher) platform that leverages viscoelastic fluids, i.e. , methylcellulose (MC) solutions, and mechanoporation for highly efficient robust intracellular mRNA delivery.

In this work, a low power and robust electroactive microwell-based implantable drug delivery system, intended for use with autonomous microsystems, is presented. The device comprises of an upper silicon based structure in which the storage sites are defined lower electrically functionalized PDMS (polydimethylsiloxane) backing. ejection mechanism developed here exploits localized electrokinetic effects to control both release time rate chemicals stored independent well sites. It shown how can...

Effective tumor regression has been observed with chimeric antigen receptor (CAR) T cells; however, the development of an affordable, safe, and effective CAR-T cell treatment remains a challenge. One major obstacles is that suboptimal genetic modification cells reduces their yield antitumor activity, necessitating next-generation engineering approach. In this study, we developed nonviral nanoengineering system allows highly efficient delivery diverse functional nanomaterials into primary...

Abstract Probing the kinetic evolution of nanoparticle (NP) growth in liquids is essential for understanding complex nano‐phases and their corresponding functions. Terahertz (THz) sensing, an emerging technology next‐generation laser photonics, has been developed with unique photonic features, including label‐free, non‐destructive, molecular‐specific spectral characteristics. Recently, metasurface‐based sensing platforms have helped trace biomolecules by overcoming low THz absorption...