A5024505007- Ion channel regulation and function
- Venomous Animal Envenomation and Studies
- Nicotinic Acetylcholine Receptors Study
- Reconstructive Facial Surgery Techniques
- Antimicrobial Peptides and Activities
- CRISPR and Genetic Engineering
- Microbial Metabolic Engineering and Bioproduction
- Bacterial Genetics and Biotechnology
- Insect and Pesticide Research
- MicroRNA in disease regulation
- Reconstructive Surgery and Microvascular Techniques
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Cleft Lip and Palate Research
- RNA modifications and cancer
- Congenital Ear and Nasal Anomalies
- Genomics and Chromatin Dynamics
- Biomedical and Engineering Education
- Metabolomics and Mass Spectrometry Studies
- Developmental Biology and Gene Regulation
- Fungal and yeast genetics research
- Lipid Membrane Structure and Behavior
- Cancer Mechanisms and Therapy
- Cancer-related molecular mechanisms research
- Advanced Chemical Sensor Technologies
Jilin University
2024
Hunan University of Science and Technology
2019-2023
Beijing Jiaotong University
2023
Heilongjiang University of Chinese Medicine
2021-2022
National University of Defense Technology
2011-2021
Zhejiang University
2020-2021
China Southern Power Grid (China)
2020
Lanzhou University
2019
Capital Normal University
2018
Beijing Institute of Water
2018
Although ultraviolet (UV) light illumination has been widely used to increase the sensitivity of semiconductor gas sensors, its underlying mechanism is still blurred and controversial. In this work, influence UV on ZnO nanofilm sensors explored experimentally simulated based a modified Wolkenstein's model. The influential factors are determined respectively: surface band bending Fermi level measured by Kelvin probe force microscopy, binding energy extrinsic state calculated density...
Beijing is severely affected by land subsidence, and rapid urbanisation building construction might accelerate the subsidence process. Based on 39 Envisat Advanced Synthetic Aperture Radar (ASAR) images acquired between 2003–2010, 55 TerraSAR-X 2010–2016, urban information, we analysed relationship buildings at regional, block, scales. The results show that surface displacement rate in area ranged from −109 mm/year to +13 −151 +19 2010–2016; two bowls were mainly distributed eastern part of...
Abstract Natural composite biomaterials are good structural supports for bone cells to regenerate lost bone. Here, we report that a chitosan-collagen film accelerated osteoblast proliferation, differentiation and matrix mineralization in MC3T3-E1 cells. Intriguingly, observed the enhanced phosphorylation of Erk1/2. We showed increased transcriptional activity Runx2, which is an important factor regulating downstream phosphorylated Consistent with this observation, found expression...
Formation of the gastrula organizer requires suppression ventralizing signals and, in fish and frog, need to counteract effect ubiquitously present maternal factors that activate expression Bmps. How balance between dorsalizing is shifted towards establishment at late blastula stages not well understood. Mutations zebrafish bozozok (boz) cause severe defects axial mesoderm anterior neurectoderm affect formation. The boz gene encodes homeodomain protein Bozozok/Dharma its region activated...
The molecular basis for the embryonic and perinatal clinical forms of biliary atresia is largely undefined. In this study, we aimed to: 1) determine if can be differentiated at transcriptional level, 2) search mechanisms underlying phenotypic differences. To end, generated biotinylated cRNA probes from livers age-matched infants with (n = 5) 6) time diagnosis hybridized them against Affymetrix human HG-U133 A B microarrays containing 44,760 gene products. Data filtering two-way cluster...
Let-7 miRNAs are reported to play an inhibitory role in carcinogenesis, tumor progression, recurrence, and pluripotency of cancer. However, few studies have the relationship between let-7 drug sensitivity, especially for let-7a (a subtype let-7). This study aimed investigate function regulating sensitivity hepatocellular carcinoma (HCC) cell lines cetuximab.The cytotoxicity cetuximab on HCC (Huh7, Hep3B, HepG2, SNU449, SNU387) was evaluated using a viability assay (the Cell Counting Kit-8...
Microgravity is a major environmental factor of space flight that triggers dysregulation the immune system and increases clinical risks for deep-space-exploration crews. However, systematic studies molecular mechanisms adverse effects microgravity on in animal models are limited. Here, we establish ground-based zebrafish disease model research immunology. RNA sequencing analysis demonstrates retinoic-acid-inducible gene (RIG)-I-like receptor (RLR) Toll-like (TLR) signaling pathways...
The Drosophila morphogenetic protein Bicoid (Bcd) is a homeodomain-containing activator that stimulates the expression of target genes during early embryonic development. We demonstrate small domain Bcd located immediately N-terminally homeodomain represses its own activity in cells. This domain, referred to as self-inhibitory works an independent module does not rely on any other sequences and can repress heterologous activators. further show this affect properties DNA binding or...
Abstract Here, an efficient cloning strategy for large DNA fragments and simultaneous assembly of multiple is presented. This named OEPR (based on O verlap E xtension P CR R ecombination in vivo ). a seamless, restriction- ligation-independent method. The method takes advantage both homologous recombination enzymes E. coli overlap PCR. Using cloning, long fragment (1–6 kb) or (2–4 fragments) can be easily constructed simultaneously assembled into target vector.
Tea plants that have a large leaf area mainly suffer from heavy metal accumulation in the above-ground parts through foliar uptake. With world rapid industrialization, this pollution tea is considered crucial challenge due to its potential health risks. The present study proposes an innovative approach based on visible and near-infrared (Vis-NIR) spectroscopy coupled with chemometrics for characterization of chemical indicators under airborne lead stress, which can be performed fast situ....
Recently, there are significant advancements in learning-based image compression methods surpassing traditional coding standards. Most of them prioritize achieving the best rate-distortion performance for a particular rate, which limits their flexibility and adaptability various applications with complex varying constraints. In this work, we explore potential resolution fields scalable propose reciprocal pyramid network (RPN) that fulfills need more adaptable versatile compression....
The coding sequence of huwentoxin-I, a neurotoxic peptide isolated from the venom Chinese spider Ornithoctonus huwena, was amplified by PCR using cDNA library constructed glands. cloned fragment inserted into expression vector pET-40b and transformed E. coli strain BL21 (DE3). soluble fusion protein, disulfide interchange protein (DsbC)-huwentoxin-I, auto-induced in periplasm absence IPTG. After partial purification Ni-NTA column, expressed digested enterokinase to release heteroexpressed...
Abstract For years, prokaryotic hosts have been widely applied in bio-engineering. However, the confined vivo enzyme clustering of heterologous metabolic pathways these organisms often results low local concentrations enzymes and substrates, leading to a productive efficacy. We developed new method accelerate system by integrating transcription activator-like effector (TALE)-based scaffold into an Escherichia coli chassis. The binding abilities TALEs artificial DNA were measured through...
Hepatocellular carcinoma (HCC) is one of the most widespread malignant human tumors worldwide. Treatment options include radiotherapy, surgical intervention and chemotherapy; however, drug resistance an ongoing treatment concern. In present study, effects a microRNA (miR/miRNA), miR-9, on sensitivity HCC cell lines to epidermal growth factor receptor inhibitor, cetuximab, were examined. miR-9 has been proposed serve role in tumorigenesis tumor progression. bioinformatics analyses identified...