Abstract Niemann-Pick disease type C protein 1 (NPC1), classically associated with cholesterol transport and viral entry, has an emerging role in cancer biology. Here, we demonstrate that knockout of Npc1 hepatocytes attenuates hepatocellular carcinoma (HCC) progression both DEN (diethylnitrosamine)-CCl 4 induced MYC-driven HCC mouse models. Mechanistically, NPC1 significantly promotes by modulating the TGF-β pathway, independent its traditional transport. We identify 692-854 amino acid...

In this study, novel saltiness/saltiness enhancing peptides VESQTNGIIR, NQITKPNDVY, and DEDTQAMP were identified from the hen egg proteins via virtual enzymatic, molecular docking, electronic tongue analysis. Their saltiness enhancement effect was analyzed by e-tongue Saltiness rates of peptide VESQTNGIIR 47.24 %, 95.28 % 105.94 those NQITKPNDVY 32.40 70.16 71.25 at salt reduction concentrations 25 35 45 respectively. 5.83 11.24 concentrations, Molecular docking demonstrated that Glu286,...

Hypercytokines cause acute respiratory distress syndrome (ARDS) in coronavirus disease 2019 (COVID-19) patients, which is the main reason for intensive care unit treatment and leading of death COVID-19 patients. Cytokine storm a critical factor development ARDS. This study evaluated efficacy safety Oxiris filter Five patients with who received continuous renal replacement therapy (CRRT) Henan provincial people's hospital between January 23, March 28, 2020, were enrolled this study. Heart...

Sorafenib is the first‑line treatment for advanced hepatocellular carcinoma (HCC). Since many HCC patients experience drug resistance, there an urgent need to discover more effective therapeutic strategies overcome resistance. Long non‑coding RNAs (lncRNAs) play important role in tumor However, research on of lncRNA H19 sorafenib resistance quite limited. In present study, CCK‑8 assay, RT‑qPCR, EdU staining, immunofluorescence and western blot analysis were used detect effect cells....

Background Sepsis-associated acute kidney injury (S-AKI) is a critical illness and often associated with high morbidity mortality rates. The soluble urokinase-type plasminogen activator receptor (suPAR) an important immune mediator involved in injury. However, its diagnostic value S-AKI patients remains unclear. Therefore, we assessed the early predictive of suPAR for patients.

Background: Acute kidney injury (AKI) is a common postoperative event. Previous research suggests that intraoperative hypotension (IOH) associated with AKI. This connection, however, has not been studied in patients undergoing pancreaticoduodenectomy. Methods: Based on retrospective cohort study, we analyzed 844 adult who had pancreaticoduodenectomy between December 2016 and June 2020 Henan Provincial People's Hospital. We graphically modeled the associations lowest systolic diastolic...

Abstract BACKGROUND This study aimed to reduce salt intake without compromising food sensory properties. Novel salt‐enhancing peptides were identified from crocodile hemoglobin via virtual screening and evaluated for their salt‐reducing effects using molecular docking, electronic tongue analysis, dynamics simulations. RESULTS A total of 24 water‐soluble non‐toxic obtained by enzymolysis. The protein structure human transmembrane channel‐like 4 (TMC4), a novel taste receptor, was constructed...

Background: Pulmonary arterial hypertension (PAH) represents a substantial global public health burden, particularly among women of reproductive age, who face significant risks during pregnancy if affected by the condition. This study seeks to provide thorough evaluation disease burden and trends PAH in age (WPAH), aiming guide development targeted prevention control strategies. Methods: Data on incidence, prevalence, disability-adjusted life years (DALYs), deaths WPAH were extracted from...

Let-7 miRNAs are reported to play an inhibitory role in carcinogenesis, tumor progression, recurrence, and pluripotency of cancer. However, few studies have the relationship between let-7 drug sensitivity, especially for let-7a (a subtype let-7). This study aimed investigate function regulating sensitivity hepatocellular carcinoma (HCC) cell lines cetuximab.The cytotoxicity cetuximab on HCC (Huh7, Hep3B, HepG2, SNU449, SNU387) was evaluated using a viability assay (the Cell Counting Kit-8...

We conducted a case-control study to investigate the genetic variants Interleukin-1β(IL-1β) +3953 C/T (rs1143634), IL-6 -174G/C (rs1800795), IL-8 -251T/A (rs4073), and IL-10 -1082A/G (rs1800896) -819C/T (rs1800871) in development of coronary artery disease (CAD). A total 410 individuals with CAD were enrolled between January 2012 December 2014. Genotyping five gene polymorphisms was performed using polymerase chain reaction combined restriction fragment length polymorphism methodology. By...

Immunotherapy has revolutionized the treatment of clear cell renal carcinoma (ccRCC). However, therapy is constrained by drug resistance. Therefore, further characterization immune infiltration in ccRCC needed to improve its efficacy.Here, we adopted CIBERSORT method analyze level 22 cells, and analyzed correlation cells clinical parameters The Cancer Genome Atlas. We used consensus clustering cluster identified differently expressed genes (DEGs) between hot cold tumors using "Limma"...

Hepatocellular carcinoma (HCC) is one of the most widespread malignant human tumors worldwide. Treatment options include radiotherapy, surgical intervention and chemotherapy; however, drug resistance an ongoing treatment concern. In present study, effects a microRNA (miR/miRNA), miR-9, on sensitivity HCC cell lines to epidermal growth factor receptor inhibitor, cetuximab, were examined. miR-9 has been proposed serve role in tumorigenesis tumor progression. bioinformatics analyses identified...

The characteristics of mesenchymal stem cells (MSCs) in the repair acute kidney injury (AKI) have been extensively studied. However, some potential molecular mechanisms remain indistinct. aim this study was to combine published microRNA (miRNA) transcriptional profiling with quantitative proteomic analyses reveal specific miRNAs or genes for MSC-based therapy AKI.Transcriptome data containing significantly changed renal tissue from AKI mice treated and without MSCs were downloaded....

The aim of this study was to explore the effect atorvastatin intervention on plasma N-terminal pro-B-type natriuretic peptide (NT-proBNP) and inflammatory cytokine levels in patients with heart failure (HF). One hundred twenty-three HF were selected from our hospital randomly divided into control (N = 61) observation 62) groups; former received conventional treatment, while latter given treatment combined atorvastatin. Plasma NT-proBNP, cytokines [high-sensitive C-reactive protein (hs-CRP),...

Aristolochic acid (AA) exposure is a severe public health concern worldwide. AAs damage the kidney with an inevitable acute phase that similar to injury (AKI). Gasdermin E (GSDME) abundant in kidney; thus; it-mediated pyroptosis might be essential connecting cell death and inflammation promoting AAs-AKI. However, role exact mechanism of AAs-AKI have not been investigated. In this study, aristolochic I (AAI) was used establish AKI models. The expression translocation results showed...

Objective: To estimate the prevalence and associated factors of adult female urinary incontinence in Hebei province. Methods: Stratified multistage sampling method was used, between January 2016 to May 2016, investigate target population While, logistic regression used analyse datas. Results: A population-based survey conducted 2 450 women province, there were 408 effective questionnaires after deleting 48 invalid questionnaires. According results, average age subjects (56±15) years old,...

Abstract Macrophages play an important role in the pathogenesis of systemic lupus erythematosus–associated diffuse alveolar hemorrhage (DAH). The immunomodulation macrophage responses might be a potential approach for prevention and treatment DAH. Erythropoietin (EPO) could regulate bioactivities by binding to EPO receptor expressing on macrophages. This study assessed effects DAH protection using immune-mediated murine model with macrophages as major contributor. A was established female...

Cisplatin is widely used as a chemotherapeutic drug to treat various solid tumors. However, it often induces severe side effects, including nephrotoxicity, which limits its application in clinical settings. Furthermore, the underlying mechanisms of action are unclear. Here, we applied whole-transcriptome RNA sequencing cisplatin-induced acute kidney injury (CP-AKI) mouse model evaluate competing endogenous (ceRNA) networks. We found 4460 mRNAs, 1851 long non-coding RNAs, 101 circular and 102...