A5103269428- Sphingolipid Metabolism and Signaling
- Plant tissue culture and regeneration
- Pancreatic function and diabetes
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Transgenic Plants and Applications
- Adenosine and Purinergic Signaling
- Cholesterol and Lipid Metabolism
- Pancreatitis Pathology and Treatment
- Plant Molecular Biology Research
- Nicotinic Acetylcholine Receptors Study
- Antimicrobial Peptides and Activities
- Plant-Microbe Interactions and Immunity
- Plant Virus Research Studies
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Metabolism, Diabetes, and Cancer
- Cancer-related molecular mechanisms research
- Phagocytosis and Immune Regulation
- Lipid metabolism and disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Infectious Diseases and Tuberculosis
- Sirtuins and Resveratrol in Medicine
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Plant Gene Expression Analysis
Second Affiliated Hospital of Nanjing Medical University
2010-2024
Hangzhou Xixi hospital
2023
Yueyang Hospital
2023
Shanghai University of Traditional Chinese Medicine
2023
Nanjing Medical University
2008-2018
Guilin Medical University
2012-2016
West China Medical Center of Sichuan University
2015
Jiangsu Province Hospital
2008
Salk Institute for Biological Studies
1993-2008
DuPont (United States)
2002
<i>Background:</i> Recent studies suggest the involvement of adenosine monophosphate-activated serine/threonine protein kinase (AMPK) pathway in pathogenesis diabetic nephropathy (DN). Resveratrol, an agent that activates AMPK, may have potential to protect against development DN. This study was designed investigate therapeutic effects resveratrol on renal hypertrophy early-stage diabetes and underlying mechanisms. <i>Method:</i> Molecular structural changes involved...
Preoperative peripheral blood neutrophil-to-lymphocyte ratio (NLR) has been proposed to predict prognosis of hepatocellular carcinoma (HCC). However, the cutoff value NLR in several studies is not consistent. This study aims investigate correlation preoperative with clinicopathologic features and patients who have undergone resection for HCC.Clinical data 256 HCC underwent radical hepatectomy were retrospectively analyzed. The divided into low-NLR group (NLR ≤ 2.31) high-NLR > 2.31). A...
Diabetic nephropathy (DN) is an important microvascular complication of diabetes, and one the leading causes end‑stage kidney disease. However, mechanism DN pathogenic process remains unclear. Recently, long non‑coding (lnc)RNA dysregulation has been regarded to cause occurrence development various human diseases, although functions lncRNAs in are poorly understood. The authors' previous study using microarray analysis identified hundreds dysregulated DN, these were not demonstrated. Out...
Summary A 1.5 kb promoter fragment from the rice ( Oryza sativa L.) RCH10 gene, which encodes a basic endochitinase inducible by wounding and fungal elicitor, was translationally fused to β‐glucuronidase (GUS) reporter gene transferred tobacco Agrobacterium tumefaciens ‐mediated leaf disc transformation. Wounding of leaves induced GUS activity low basal levels, addition elicitor wounded tissue caused further marked activation fusion. During vegetative development high levels were observed in...
It has been reported that the effect of inflammatory cytokines on β‐cell destruction in type 1 diabetes is concentration‐dependent. However, underlying mechanisms remain unclear. In present study, we found a high concentration promoted apoptosis rat line INS ‐1, whereas low had no effect. We also at stimulated neutral ceramidase ( NCD ase) release via exosomes from ‐1 cells, inhibited ase release. Furthermore, results showed ase‐containing isolated culture medium cells treated with induced...
Aims: To investigate the effects of neutral ceramidase (NCDase) packaged in exosomes that are secreted from β-cells on free fatty acid (FFA)-induced apoptosis and its role regulation sphingolipid-mediated signaling pathway. Methods: HPLC Western blotting were performed to determine NCDase activity expression. Annexin V-fluorescein-isothiocyanate/propidium iodide flow cytometry was used assess apoptosis. Electrospray ionization tandem mass spectrometry for ceramide (Cer),...
Free fatty acids (FFA) can have deleterious effects on β-cells and promote type 2 diabetes, a process known as lipotoxicity. Recently, the induction of endoplasmic reticulum (ER) stress is one mechanism proposed to contribute detrimental FFA β-cells. Tauroursodeoxycholic acid (TUDCA) has been reported show cytoprotective by alleviating ER induced some cytotoxic stimuli. The aim this study was investigate TUDCA (palmitate)-induced apoptosis in rat islet pancreatic β-cell line INS-1 cultured...
Oversupply of free fatty acids such as palmitic acid (PA) from the portal vein may cause liver insulin resistance. Production reactive oxygen species plays a pivotal role in PA-induced resistance H4IIEC3 hepatocytes. Recently, we found that exosomes secreted INS-1 cells were transfected with neutral ceramidase (NCDase) plasmids had raised NCDase activity; these NCDase-enriched could inhibit cell apoptosis. Here, showed PA reduced insulin-stimulated tyrosine phosphorylation receptor substrate...
Ceramidase hydrolyzes ceramide and produces sphingosine as a substrate of kinase (SPHK), which transforms to sphingosine-1-phosphate. It has been reported that cytokines elicit SPHK activation in rat beta-cells. As provider, ceramidase should also be activated. In our previous work, we showed the increase mRNA protein levels cytokine-treated INS-1 beta-cells resulted chronic neutral ceramidase. Here found acid (AC) is activated by at an early stage via tyrosine phosphorylation. addition,...
Neutral ceramidase (NCDase) is a class of ceramidases, key enzyme in ceramide degradation. Recently, it was observed that NCDase activity suppressed by saturated fatty acids to increase content rat muscle. However, little known about its changes and roles palmitate (Palm)-induced lipotoxicity pancreatic β cells. Here, we demonstrated Palm treatment significantly down-regulated activity, mRNA protein levels INS-1 In addition, caused significant accumulation ceramide, while SPH level remained...
High concentrations of free fatty acids (FFAs) or lipopolysaccharide (LPS) could lead to β-cell apoptosis and dysfunction, while low-grade elevation FFAs LPS, which are more common in people with type 2 diabetes mellitus (T2DM) obesity, have no obvious toxic effect on β-cells. Palmitate is a component closely related metabolic disorders FFAs. Recent studies found that palmitate LPS synergistically affects the sphingolipid signaling pathway by activating Toll-like receptor 4 (TLR4) further...
We characterized rice cDNA sequences for OsDr1 and OsDrAp1, which encode structural homologs of the eukaryotic general repressors Dr1 DrAp1, respectively. OsDrAp1 are nuclear proteins that interact with each other TATA binding protein/DNA complex. In vitro in vivo functional analyses showed functions as a repressor, unlike its role systems, DrAp1 is corepressor. functioned together much stronger repressor than either one alone. Functional dissections revealed N-terminal histone-fold domains...
Polymorphisms in the nicotinic acetylcholine receptor subunit CHRNA5 gene have been associated with lung cancer positive susceptibility European and American populations. In present hospital-based, case-control study, we determined whether polymorphism rs503464 of is risk Chinese individuals. A single nucleotide rs503464, c.-166T>A (hereafter T>A), was identified using TaqMan-MGB probes sequencing via PCR 600 cases healthy Genotype frequencies for (T>A) were Hardy-Weinberg equilibrium...
Summary Approximately 40% of middle‐aged men exhibit symptoms late‐onset hypogonadism ( LOH ). However, the mechanism androgen deficiency is still currently unclear. As shown in our previous studies, SET protein expressed testicular Leydig cells and ovarian granule cells. This study was designed to investigate effect on production The Ad CMV / AdH1si RNA adenoviruses were individually transduced into a cultured mouse cell line mLTC ‐1) with or without human chorionic gonadotropin HCG )...
Rationale: Endoscopic injection sclerotherapy (EIS) is a common treatment for patients with liver cirrhosis and esophageal varices. It can effectively treat variceal rupture bleeding caused by cirrhosis. However, EIS has many complications, including postoperative bleeding, retrosternal pain, ulcers, stenosis, ectopic embolism. Intramural hematoma of the esophagus (IHE) rare complication that lead to chest tightness, dysphagia. Patient’s concerns: A 55-year-old man developed severe nausea...
Background: Thalassemia is a hereditary blood disease resulting from globin chain synthesis impairment because of α- and/or β-globin gene variants. α-thalassemia characterized by non-deletional and deletional variants in the HBA locus, which rare are difficult to detect conventional polymerase reaction (PCR)-based methods. Case report: We report case one-month-old boy, who his mother had abnormal hematological parameters, while father normal hematology. Conventional PCR-reverse dot blot...