A5102750923- Peroxisome Proliferator-Activated Receptors
- Lipid metabolism and biosynthesis
- Liver Disease Diagnosis and Treatment
- Cholesterol and Lipid Metabolism
- Adipose Tissue and Metabolism
- Cancer, Lipids, and Metabolism
- Pancreatic function and diabetes
- Lipid metabolism and disorders
- Animal Nutrition and Physiology
- Diabetes and associated disorders
- Glycosylation and Glycoproteins Research
- Cardiovascular Disease and Adiposity
- Biochemical effects in animals
- Genomics and Chromatin Dynamics
- Liver physiology and pathology
- Gallbladder and Bile Duct Disorders
- Congenital heart defects research
- Sphingolipid Metabolism and Signaling
- Pancreatitis Pathology and Treatment
- Animal Genetics and Reproduction
- Hormonal Regulation and Hypertension
- Estrogen and related hormone effects
- Drug Transport and Resistance Mechanisms
- Bone Tissue Engineering Materials
- Sirtuins and Resveratrol in Medicine
Guilin Medical University
2021-2022
Sichuan Agricultural University
2009-2020
Soochow University
2019
Cornell University
2014
Dongguan People’s Hospital
2009
The molecular underpinnings of β-cell dysfunction and death leading to diabetes are not fully elucidated. objective the current study was investigate role endoplasmic reticulum-associated degradation (ERAD) in pancreatic β-cells. Chemically induced ERAD deficiency rat insulinoma cell line INS-1 markedly reduced glucose-stimulated insulin secretion (GSIS). mechanistic basis for this effect studied cells mice lacking as a consequence genetic ablation core protein SEL1L. Targeted disruption...
Abstract The effect of glucosylceramide (GlcCer) reprogramming on liver cancer metastasis remains poorly understood. In this study, we demonstrated that the protein expression GBA1, which catalyses conversion GlcCer to ceramide, was downregulated in tissue. A clinical relevance analysis revealed low GBA1 associated with metastatic potential cells. Furthermore, loss- and gain-of-function studies confirmed promoted both vitro vivo. Mechanistic indicated enhanced ability by promoting...
SUMMARY In order to obtain some information on how fatty liver arises in geese, we investigated the role of insulin and glucose triglyceride (TG) accumulation goose primary hepatocytes. Goose hepatocytes were isolated treated with glucose. Compared control group, 100 150 nmol l–1 increased TG accumulation, acetyl-CoA carboxylase-α (ACCα) acid synthase (FAS)activity, mRNA levels sterol regulatory element-binding protein-1(SREBP-1), FAS ACCα genes. Insulin at 200 had an inhibiting effect...
High concentrations of free fatty acids (FFAs) or lipopolysaccharide (LPS) could lead to β-cell apoptosis and dysfunction, while low-grade elevation FFAs LPS, which are more common in people with type 2 diabetes mellitus (T2DM) obesity, have no obvious toxic effect on β-cells. Palmitate is a component closely related metabolic disorders FFAs. Recent studies found that palmitate LPS synergistically affects the sphingolipid signaling pathway by activating Toll-like receptor 4 (TLR4) further...
The overaccumulation of triglycerides in hepatocytes induces hepatic steatosis; however, little is known about the mechanism goose steatosis. aim this study was to define an experimental model hepatocellular steatosis with TG and minimal cytotoxicity, using a mixture various proportions oleate palmitate free fatty acids (FFAs) induce fat-overloading, then suppressive subtractive hybridization quantitative PCR approach identify genes higher or lower expression levels after treatment cells FFA...
In this study, we investigated the role of liver X receptor (LXR) activation in hepatic assembly and secretion very low density lipoprotein-triglycerides goose primary hepatocytes. Goose hepatocytes were isolated treated with LXR agonist T0901317. Total triglyceride accumulation, intracellular extracellular concentrations, lipoprotein concentration, gene expression levels LXRα, microsomal transfer protein, acyl coenzyme A:diacylglycerol acyltransferase (DGAT) 1, DGAT2 measured We found a...
The objective of the current research was to investigate pattern subcutaneous adipose tissue growth during Peking duck (Anas platyrhynchos) early development and determine reasons for regional differences. morphological characteristics in 5 regions tissue, including neck area (NSF), chest (CSF), lower abdomen (ASF), back (BSF), leg (LSF), were analyzed by comparing morphology sections, adipocyte volume number, lipid content from wk 1 8. Moreover, mRNA expression several molecular marker...
Liquid crystals (LCs) are appealing biomaterials for applications in bone regenerative medicine due to their tunable physical properties and anisotropic viscoelastic behavior. This study reports a novel composite poly (L-lactide) (PLLA) scaffold that is manufactured by simple electrospinning biomineralization technique precisely controls the fibrous structure liquid LC phase. The enriched-LC composites have superior mineralization ability than neat PLLA; furthermore BMSC cells were...
The aim of our study was to evaluate the effect overfeeding on mRNA expression levels genes involved in lipogenesis, order understand mechanism hepatic steatosis goose. Using Landes geese (Anser anser) and Sichuan White cygnoides) as experimental animals, we quantified lipogenic genes, acetyl-CoA carboxylase-α (ACCα) fatty acid synthase (FAS), two transcription factors, sterol regulatory element-binding proteins-1 (SREBP-1) carbohydrate responsive protein (ChREBP) by real-time polymerase...
The study was to research the gene expression of lipoprotein lipase (LPL) in abdomen adipose tissue, subcutaneous pectoral muscle, skeletal muscles, liver, brain and spleen geese, effect insulin glucose on LPL measured primary hepatocytes Sichuan white geese (Anser cygnoides) Landes anser). We quantified by competitive reverse transcriptase PCR (RT-PCR). results indicated: First, 0-150nM could increase mRNA level a dose-dependent pattern, 200nM had an inhibiting effect. Second, there...
In this study, the effect of oleic oil on cytoactive, triglycerides (TG) accumulation, lipids drop and mRNA expression peroxisome proliferator-activated receptor alpha (PPARalpha) gamma ( PPARgamma ) was measured in primary goose hepatocytes. Results were as follows: 1.5 mmol/L had an inhibiting cell viability; could induce hepatocytic steatosis increase TG content lipid droplet. Oleic all level PPARalpha; 0.5 PPARgamma, but 1.0 no evident effect. Conclusion: model induced steatosis, change...
Our laboratory has previously found insulin resistance and obesity in mice overexpressing Se‐dependent glutathione peroxidase‐1 (GPX1‐OE). The present study was conducted to determine if how hepatic lipid metabolism related these metabolic phenotypes the GPX1‐OE mice. Liver samples were collected from male wild‐type at ages of 3 wk 2, 6, 9, 12 mo (n = 5‐10, fed an Se‐adequate Torula yeast‐sucrose diet) profiles mRNA protein levels factors involved triglyceride synthesis, deposition,...
Angiopoietin-like protein 3 and -4 (ANGPTL-3 -4) are generally related to lipid metabolism as well angiogenesis in animals, however very less was known about their mRNA expression characterizations tissue development. In this study, the expressions of ANGPTL-3 ANGPTL-4 distribution Peking duck (Anas platyrhynchos) 1 8 weeks age were analyzed using quantitative real-time PCR methods. It observed that mRNAs broadly expressed liver adipose tissues most abundant intra-abdominal (AD). had...