A5083355160- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Genomics and Phylogenetic Studies
- Metabolism and Genetic Disorders
- Nerve injury and regeneration
- Cancer, Stress, Anesthesia, and Immune Response
- Chromosomal and Genetic Variations
- Bacterial Genetics and Biotechnology
- Protein Kinase Regulation and GTPase Signaling
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- 14-3-3 protein interactions
- Mitochondrial Function and Pathology
- Animal Genetics and Reproduction
- Neuroscience and Neuropharmacology Research
- Neurogenesis and neuroplasticity mechanisms
- Bacteriophages and microbial interactions
- Heat shock proteins research
- Signaling Pathways in Disease
- Plant Molecular Biology Research
- Lysosomal Storage Disorders Research
- PI3K/AKT/mTOR signaling in cancer
- Axon Guidance and Neuronal Signaling
- Cancer Cells and Metastasis
- Bacillus and Francisella bacterial research
University of Miami
2006-2023
Sylvester Comprehensive Cancer Center
2023
University of Miami Hospital
2021-2023
Neurological Surgery
2010-2022
Dr. John T. Macdonald Foundation
2007
Foundation Center
2007
California Institute of Technology
1992-1996
Lawrence Livermore National Laboratory
1994
A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC (for artificial chromosome) is based on Escherichia coli its single-copy plasmid F factor. It capable maintaining human genomic DNA fragments greater than 300 kilobase pairs. Individual clones appear to be maintained with a high degree structural stability in the host, even after 100 generations serial growth. Because efficiency, easy manipulation cloned DNA, stable maintenance inserted may...
The mammalian target of rapamycin (mTOR) positively regulates axon growth in the central nervous system (CNS). Although regeneration and functional recovery from CNS injuries are typically limited, knockdown or deletion PTEN, a negative regulator mTOR, increases mTOR activity induces robust regeneration. It has been suggested that inhibition S6 kinase 1 (S6K1, gene symbol: RPS6KB1), prominent target, would blunt mTOR's positive effect on growth. In contrast to this expectation, we...
Acetyltransferase p300 is essential for cardiac development and thought to be involved in myocyte growth through MEF2- GATA4-dependent transcription. However, the importance of modulation vivo unknown.Pressure overload induced by transverse aortic coarctation, postnatal physiological growth, human heart failure were associated with large increases p300. Minimal transgenic overexpression (1.5- 3.5-fold) striking hypertrophy. Both mortality mass directly related protein dosage. Heterozygous...
Nitric oxide (NO) induces apoptosis in cardiac myocytes through an oxidant-sensitive mechanism. However, additional factors appear to modulate the exact timing and rate of NO-dependent apoptosis. In this study, we investigated role mitogen-activated protein kinases (MAPKs) (extracellular signal-regulated kinase [ERK] 1/2, c-Jun N-terminal [JNK] p38MAPK) NO-mediated apoptotic signaling. The NO donor S:-nitrosoglutathione (GSNO) induced caspase-dependent neonatal rat myocytes, preceded by a...
In a neuronal overexpression screen focused on kinases and phosphatases, one "hit" was the dual specificity tyrosine phosphorylation-regulated kinase (Dyrk4), which increased number of dendritic branches in hippocampal neurons. Overexpression various Dyrk family members primary neurons significantly changed morphology. Dyrk1A decreased axon growth, Dyrk3 Dyrk4 branching, Dyrk2 both dendrite growth branching. Kinase-deficient mutants revealed that most these effects depend activity. Because...
Certain Bacillus licheniformis strains isolated from oil wells have been shown to produce a very effective biosurfactant, lichenysin A, which is structurally similar another less active lipopeptide, surfactin. Surfactin, like many small peptides in prokaryotes and lower eukaryotes, synthesized non-ribosomally by multi-enzyme peptide synthetase complex. Analysis of several synthetases bacterial fungal origin has revealed high degree sequence conservation. Two 35-mer oligonucleotides derived...
CD97, an adhesion G-protein coupled receptor highly expressed in glioblastoma (GBM), consists of two noncovalently bound domains: the N-terminal fragment (NTF) and C-terminal fragment. The contains a GPCR domain that couples to Gα12/13, while NTF interacts with extracellular matrix components other receptors. We investigated effects changing CD97 levels its function on primary patient-derived GBM stem cells (pdGSCs) vitro vivo. created functional mutants: constitutively active ΔNTF...
Abstract Background The median survival of Glioblastoma multiforme (GBM) patients is 14+ months due to poor responses surgery and chemoradiation. Means counteract radiation resistance are therefore highly desirable. We demonstrate the membrane bound matrix metalloproteinase MT1-MMP promotes GBM radiation, that using a selective brain permeable inhibitor, (R)-ND336, improved tumor control can be achieved in preclinical studies. Methods Public microarray RNA-sequencing data were used determine...
INTRODUCTION: Increased levels of adhesion G Protein-Coupled Receptor (GPCR) CD97 have been detected in multiple metastatic cancers. Glioblastoma (GBM), a Grade-IV Glioma, also overexpress this receptor, and its positively correlate with more aggressive GBM invasion. METHODS: consists two distinct domains: the large extracellular fragment interacting ECM other cell surface receptors GPCR core that activates heterotrimeric Proteins G12/13. To investigate mechanism action CD97, we generated...
To understand the phosphorylation events underlying axon/dendrite growth, we screened a cDNA library of kinases and phosphatases by overexpression in primary hippocampal neurons. One hits was d ual specificity tyrosine ( Y ) phosphorylation‐ r egulated k inase, Dyrk4. Overexpression Dyrk4 increased both number neurites dendritic branches. A close relative Dyrk4, Dyrk1A, branching greatly affected morphology growth cones. Moreover, several members Dyrk family (Dyrk1A, Dyrk2 Dyrk3) perturbed...
Abstract The main obstacles for effective treatment of Glioblastoma (GBM) are high invasiveness and heterogeneity. Each GBM tumor is composed multiple genetically distinct subpopulations Glioma Stem Cells (GSCs), exhibiting cell plasticity affecting their invasive proliferative properties. Treatment interventions changes in microenvironment force GSCs to adapt through gene expression that supports therapeutic resistance. Expression level the surface receptor CD97/ADRGE5 positively correlates...