A5101937573- Virus-based gene therapy research
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Plant Stress Responses and Tolerance
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Plant Micronutrient Interactions and Effects
- Animal Disease Management and Epidemiology
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 detection and testing
- Ferroelectric and Piezoelectric Materials
- Mycobacterium research and diagnosis
- Parkinson's Disease Mechanisms and Treatments
- Ion channel regulation and function
- Dielectric materials and actuators
- Medical Research and Treatments
- Animal Virus Infections Studies
- Mosquito-borne diseases and control
- Virology and Viral Diseases
- Neurological Disease Mechanisms and Treatments
- Pain Mechanisms and Treatments
- Sinusitis and nasal conditions
- Aquaculture disease management and microbiota
- Neurobiology and Insect Physiology Research
First Affiliated Hospital of Guangzhou Medical University
2018-2023
State Key Laboratory of Respiratory Disease
2016-2023
Guangzhou Medical University
2016-2023
Nanjing Agricultural University
2007-2023
Guangzhou Experimental Station
2023
Guangdong University of Technology
2023
Jishou University
2019
Nanjing University of Chinese Medicine
2019
Pingdingshan University
2013
Xian Yang Central Hospital
2013
Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by lack of an animal model permissive virus. Here, we report in vitro and vivo HAdV infections tree shrews, closest relative primates. HAdV-3, -7, -14, -55 efficiently replicated primary cell cultures. After intranasal inoculation shrews with HAdV-55, viral replication oropharyngeal region remained high until day 5 post-infection was still...
New SARS-CoV-2 variants continue to prevail worldwide, and effective vaccines are needed prevent an epidemic. mRNA gradually being applied the prevention control of infectious diseases with significant safety effectiveness. The spike (S) protein is main target vaccine design, but impact signal peptide (SP), transmembrane region (TM), cytoplasmic tail (CT) on remains unclear. In this study, we constructed three forms related S protein: full-length, deletion TM CT, simultaneous SP, compared...
Manganese (Mn) is demonstrated to be essential for plants. Ion homeostasis maintained in plant cells by specialized transporters. PbMTP8.1, which encodes a putative Mn-CDF transporter Pyrus bretschneideri Rehd, was expressed mainly leaves and complemented the Mn hypersensitivity of Mn-sensitive yeast mutant △pmr1 previous research conducted our laboratory. In present study, we report that expression PbMTP8.1 can enhance tolerance accumulation Saccharomyces cerevisiae. Subcellular...
Grass carp reovirus (GCRV) of the genus Aquareovirus and family Reoviridae causes a severe hemorrhagic disease in grass fingerlings China. GCRV genome is composed 11 double-stranded RNA segments, which segment 8 encodes major core capsid protein VP6. In this study, VP6 gene following an RT-PCR-amplification from 104 strain was cloned into expression vector pET-32a to obtain pET-32(a)-VP6. The expressed Escherichia coli BL21 as fusion 64 kDa. After purification with HisLink Spin Protein...
In the case of dielectric energy storage devices, excessive pursuit giant electric fields means greater exposure to high temperatures and insulation damage risk. Ferroelectric thin film devices offer opportunities for needs under finite due their intrinsically large polarization advantage small size. Herein, we designed capacitor's layer by doping barium titanate Ba(1-x)CexTiO3 (BCTO). The addition highly charged ions Ce3+ induce cell shrinkage thus further optimizing ferroelectric...
The ongoing coronavirus disease 2019 (COVID-19) pandemic has a significant global social and economic impact, the emergence of new more destructive mutant strains highlights need for accurate virus detection. Here, 90 monoclonal antibodies (MAbs) that exclusively reacted with severe acute respiratory syndrome 2 (SARS-CoV-2) nucleocapsid protein (NP) were generated. These MAbs did not cross-react NPs common human coronaviruses (HCoVs, i.e., 229E, OC43, HKU1, NL63) Middle East Respiratory...
Objective To construct a recombinant human adenovirus type 3 (HAd3) vector expressing one major epitope of dengue virus 1. Methods The gene encoding the envelope protein (304-314 aa) 1 was inserted into hypervariable region (HVR1) HAd3 hexon by using overlap PCR. The cloned shuttle plasmid, then linearized with AsisⅠrestriction enzyme and co-transformed Escherichia coli BJ5183 strains digested backbone plasmid for homologous recombination. pBRAdΔE3GFP-DENV1 transfected AD293 cells to...
The ongoing coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has millions of cases infections, leading to a global health emergency. SARS-CoV-2 spike (S) protein plays the most important role in viral infection, and S1 subunit its receptor-binding domain (RBD) are widely considered attractive vaccine targets. RBD is highly immunogenic linear epitopes for development therapy, but on have rarely been reported. In this study, 151 mouse...
Objective To prepare human adenovirus type 4 (Ad4) vector expressing enhanced green fluorescence protein (EGFP). Methods This study used a previously prepared plasmid pBRAd4 containing the whole genome DNA of Ad4-GZ01 strain. The Ad4 E3 region was deleted and replaced with EGFP expression frame by conventional molecular cloning method. Then recombinant transfected into AD293 cells to rescue virus which identified sequencing, SDS-PAGE ELISA. purified virions were injected mice induced...
Objective To summarize the epidemiological data of hand, foot and mouth disease (HFMD) in China from 2013 to 2017 analyze its molecular characteristics order provide scientific basis for improving prevention control measures. Methods China National Knowledge Infrastructure, Wanfang Database China, Chinese VIP Journal Net Pubmed were used search HFMD published recent years. notification surveillance mainland obtained Health Family Planning Commission World Organization. Basic statistic...