A5009629810- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Enzyme Catalysis and Immobilization
- Aluminum toxicity and tolerance in plants and animals
- Cellular Mechanics and Interactions
- Marine Biology and Environmental Chemistry
- Marine Ecology and Invasive Species
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Plant responses to water stress
- Listeria monocytogenes in Food Safety
- Enzyme Structure and Function
- RNA modifications and cancer
- Silicon Effects in Agriculture
- Advanced Proteomics Techniques and Applications
- Soil Carbon and Nitrogen Dynamics
- Amino Acid Enzymes and Metabolism
- Microbial Inactivation Methods
- Advanced Photocatalysis Techniques
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Heavy metals in environment
- Protein purification and stability
- Food Safety and Hygiene
Institute of Mechanics
2016-2025
Chinese Academy of Sciences
2016-2025
Institute of Soil Science
2013-2025
University of Chinese Academy of Sciences
2020-2025
Nanjing University
2021-2023
Nanjing General Hospital of Nanjing Military Command
2021-2023
Harbin Engineering University
2018-2021
Agency for Science, Technology and Research
2021
First Affiliated Hospital of Henan University
2021
Bioprocessing Technology Institute
2004-2021
Tumour heterogeneity represents a major obstacle to accurate diagnosis and treatment in gastric adenocarcinoma (GA). Here, we report systematic transcriptional atlas delineate molecular cellular GA using single-cell RNA sequencing (scRNA-seq).We performed unbiased transcriptome-wide scRNA-seq analysis on 27 677 cells from 9 tumour 3 non-tumour samples. Analysis results were validated large-scale histological assays bulk transcriptomic datasets.Our integrative of identified five cell...
Abstract Background Ribosomes are essential ribonucleoprotein complexes that engaged in translation and thus indispensable for growth. Arabidopsis thaliana ribosomes composed of 80 distinct ribosomal proteins (RPs), each which is encoded by two to seven highly similar paralogous genes. Little information available on how RP genes respond a shortage mineral nutrients such as phosphate (Pi) or iron (Fe). In the present study, expression differential accumulation RPs upon Pi Fe deficiency roots...
Natural resistance-associated macrophage proteins (NRAMPs) have been shown to transport a wide range of divalent metal ions, such as manganese (Mn), cadmium (Cd), and Iron (Fe). is an essential micronutrient for plants Fe deficiency can lead chlorosis or decreased biomass. AtNRAMP6 has demonstrated the capability Cd, but its physiological function currently unclear. This study demonstrates that localized Golgi/trans-Golgi network plays important role in intracellular homeostasis flowering...
In this present study, we proposed a smart biomineralization method for creating hybrid organic–inorganic nanoflowers using Co2+-dependent enzyme (D-psicose 3-epimerase; DPEase) as the organic component and cobalt phosphate inorganic component. The prepared have many separated petals that nanometer size. Under optimum conditions (60 °C pH of 8.5), nanoflower can display its maximum activity (36.2 U/mg), which is about 7.2-fold higher than free DPEase. Furthermore, immobilized DPEase presents...
Abstract A lack of iron (Fe) inhibits the growth and development plants, leading to reduced agricultural yields quality. In last ten years, numerous studies have focused on induction Fe uptake translocation under deficiency, but regulatory mechanisms governing reutilization within plants are still not well understood. Here, we demonstrated involvement NAM/ATAF1/2/CUC2 (NAC) transcription factor NAC50 in response shortage. The content soluble was greatly nac50 mutants, increased chlorosis...
Stem cell differentiation must be regulated by intricate and complex interactions between cells their surrounding environment, ensuring normal organ tissue morphology such as the liver1. Though it is well acknowledged that microgravity provides necessary mechanical force signals for fate2, how affects growth, differentiation, communication still largely unknown due to lack of real experimental scenarios reproducibility tools. Here, Rotating Flat Chamber (RFC) was used simulate ground-based...
This study assessed the modulating effects of liquiritin against cognitive deficits, oxidative damage, and neuronal apoptosis induced by subsequent bilateral intrahippocampal injections aggregated amyloid-β1-42 (Aβ1-42). also explored molecular mechanisms underlying above phenomena. Liquiritin was orally administered to rats with Aβ1-42-induced deficits for 2 weeks. The protective on learning memory impairment Aβ1-42 were examined in vivo using Morris water maze. then euthanized further...
Background & Aims: Partial hepatectomy (PHx)-induced liver regeneration causes the increase in relative blood flow rate within liver, which dilates hepatic sinusoids and applies mechanical stretch on sinusoidal endothelial cells (LSECs). Heparin-binding EGF-like growth factor (HB-EGF) is a crucial during regeneration. We aimed to investigate whether this dilation-induced promotes HB-EGF secretion LSECs what related molecular mechanism is. Approach Results: In vivo PHx, ex perfusion vitro...
Abstract Background Phosphate (Pi) deficiency severely affects crop growth and productivity, including wheat, therefore it is necessary to develop cultivars with enhanced Pi-deficiency tolerance. However, the underlying mechanism of tolerance in wheat still elusive. Two contrasting cultivars, low-Pi tolerant Kenong199 (KN199) sensitive Chinese Spring (CS) were used reveal adaptations response Pi at morphological, physiological, metabolic, molecular levels. Results KN199 was more than CS...
Arabidopsis thaliana natural resistance-associated macrophage protein 3 (AtNRAMP3) is involved in the transport of cadmium (Cd), iron (Fe), and manganese (Mn). Here, we present a structure-function analysis AtNRAMP3 based on site-directed mutagenesis metal toxicity growth assays involving yeast mutants, combined with three-dimensional (3D) structure modeling crystal Eremococcus coleocola NRAMP family transporter, EcoDMT. We demonstrated that two conservative sites, D72 N75, are essential for...
Translocation of the dense nucleus along a gravity vector initiates mechanical remodeling cell, but underlying mechanisms cytoskeletal network and focal adhesion complex (FAC) reorganization in mammalian cell remain unclear. We quantified an MC3T3-E1 placed upward-, downward-, or edgeon-orientated substrate. Nucleus longitudinal translocation presents high value downward orientation at 24 h edge-on 72 h, which is consistent with orientation-dependent distribution perinuclear actin stress...
A robust monoclonal antibody (mAb) bioprocess requires physiological parameters such as temperature, pH, or dissolved oxygen to be well-controlled even small variations in them could potentially impact the final product quality. For instance, pH substantially affects N-glycosylation, protein aggregation, and charge variant profiles, well mAb productivity. However, relatively less is known about how jointly influences quality titer. In this study, we investigated effect of on culture...
Substrate stiffness dominates and topography assists hepatic differentiation of hESCs, the resulting HLCs exhibit hepatocyte functions repair liver injury.
Abstract An increasing number of men are fathering children at an older age than in the past. While advanced maternal has long been recognized as a risk factor for adverse reproductive outcomes, influence paternal on reproduction is incompletely comprehended. Herein, we found that miR‐125a‐5p was upregulated sperm aging males and related to inferior DNA integrity predictor. Moreover, demonstrated suppressed mitochondrial function increased cellular damage GC2 cells. We also perturbed embryo...
Keratinocyte (KC) migration in re-epithelization is crucial repairing injured skin. But the mechanisms of how mechanical stimuli regulate keratinocytes have been poorly understood.Human immortalized keratinocyte HaCaT cells were co-cultured with skin fibroblasts on PDMS membranes and transferred to static stretch device developed in-house for additional 6 day culture under mimic surface tension To detect expression proteins different position at time points effect β1 integrin...