A5011296941- biodegradable polymer synthesis and properties
- Biofuel production and bioconversion
- Diet, Metabolism, and Disease
- Enzyme Production and Characterization
- Carbon dioxide utilization in catalysis
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Microbial Metabolic Engineering and Bioproduction
- Proteins in Food Systems
- Food composition and properties
- Bacterial biofilms and quorum sensing
- Pancreatic function and diabetes
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Microplastics and Plastic Pollution
- Electrospun Nanofibers in Biomedical Applications
- Polymer crystallization and properties
- Nanocomposite Films for Food Packaging
- Polymer Foaming and Composites
- Mycotoxins in Agriculture and Food
- Peptidase Inhibition and Analysis
- Graphene and Nanomaterials Applications
- Phytase and its Applications
- Pickering emulsions and particle stabilization
- Chemical and Physical Properties in Aqueous Solutions
Sichuan Normal University
2024
Beijing Institute of Technology
2024
Changchun Institute of Biological Products
2019-2023
National Engineering Research Center for Wheat
2020
Institute of Microbiology
2018
Chinese Academy of Sciences
2018
Shanxi Medical University
2017
Jilin University
1998-1999
In this present study, we proposed a smart biomineralization method for creating hybrid organic–inorganic nanoflowers using Co2+-dependent enzyme (D-psicose 3-epimerase; DPEase) as the organic component and cobalt phosphate inorganic component. The prepared have many separated petals that nanometer size. Under optimum conditions (60 °C pH of 8.5), nanoflower can display its maximum activity (36.2 U/mg), which is about 7.2-fold higher than free DPEase. Furthermore, immobilized DPEase presents...
Lignocellulosic feedstocks have attracted much attention as a potential carbon source for lactic acid (LA) production because of their ready availability, sustainability, and renewability. However, there are at least two major technical challenges to producing LA from lignocellulose. Inhibitors derived lignocellulose pretreatment negative impact on the growth cells LA. Furthermore, pentose sugars produced difficultly utilized by most producers, which is known catabolite repression (CCR)...
Synonymous mutation of the N-terminal coding sequence (NCS) has been used to regulate gene expression. We here developed a statistical model predict effect NCSs on protein expression in Bacillus subtilis WB600. First, synonymous was performed within first 10 residues superfolder green fluorescent generate library 172 NCS mutants with different levels. A prediction then developed, which adopted G/C frequency at third position each codon and minimum free energy mRNA as independent variables,...
In this study, a series of super-toughened poly(lactic acid) (PLA)/cross-linked polyurethane (CPU) blends were prepared via one-step dynamic vulcanization PLA, polyethylene glycol (PEG) and isophorone diisocyanate trimer (IPDIT).FTIR gel tests displayed that the CPU was successfully generated during reaction.The scanning electron microscopy (SEM) images cryo-fractured impacted surfaces showed PLA/CPU had no obvious phase separation, which indicated good compatibility between PLA component...
Low expression levels and inflexible induction initiation have been the main obstacles to produce proteins using bacterial quorum sensing (QS). The typical QS system in Bacillus subtilis, ComQXPA, activates promoter PsrfA ComX ComA as an auto-inducer a activator, respectively. Here, we developed series of flexible autoinduction systems B. subtilis WB600 based on ComQXPA super-folder green fluorescent protein reporter. −35 region was replaced with corresponding conserved sequences...
The scarcity, richness, and other important physiological functions of D-psicose make it crucial to increase the yield D-psicose. production can be accomplished by 3-epimerase (DPEase) from Clostridium bolteae (CbDPEase) catalyzing substrate D-fructose. Although catalytic efficiency CbDPEase has been raised via using site-directed mutagenesis (Y68I/G109P) technique, structure-activity relationship in wild-type Y68I/G109P mutant is currently poorly understood. In our study, a battery...
Compatibilized poly (lactic acid)/poly (3-hydroxybutyrate-4-hydroxybutyrate) (PLA/P (3HB-co-4HB)) blends with (ethylene-butylacrylate-glycidyl methacrylate) (PTW) as compatibilizer were prepared by one-step melt blending in a twin-screw extruder. The mechanical properties, miscibility, phase morphology, thermal behavior, rheological behavior and degradation of the investigated. reactive epoxy groups PTW carboxyl group or hydroxyl PLA/P (3HB-co-4HB) promotes compatibility. introduction...
Computational design of pH-activity profiles for enzymes is great importance in industrial applications.
The present work simultaneously addresses the structural instability and poor diffusion kinetics of NH 4 V O 10 cathodes using Ag + intercalation, providing a new perspective on transition metal ion intercalation towards high-performance for AZIBs.
In this report, poly(lactic acid) (PLA) and Poly(butylene adipate-co-terephthalate) (PBAT) with three kinds of compatibilizers were melt blended under intensive shear flow. A self-made parallel three-screw extruder was developed to generate such flow during the process. Mechanical properties, chemical reactions among PLA, PBAT compatibilizers, rheological behavior morphology investigated. The mechanical tests showed that notched impact strength super-tough composite 10 wt% EGMA is about 20...
Abstract In this study, ionic liquid-based sugaring-out extraction was developed to separate lactic acid from the synthetic solution and actual lignocellulosic fermentation broth. Except for [E OH mim]BF 4 , ILs with BF − OTF anion can form aqueous two-phase system (ATPS) aid of saccharides. With same kind saccharides, ATPS formation ability could be promoted by increasing side-chain length in order [Hmim]BF ≈ [Bmim]BF ˃ [Emim]BF due decrease ILs’ kosmotropicity. On other hand, type ILs, an...
Abstract Blending high content of polyhydroxyalkanoates (PHAs ≥ 30 wt.%) with polylactide (PLA) provides an effective strategy to significantly improve heat resistance PLA fibers. However, it has proven challenging maintain good spinnability the PLA/PHAs blends PHAs. In this study, a series poly(L‐lactide) (PLLA)/poly[(R)‐3‐hydroxybutyrate‐ co ‐4‐hydroxybutyrate] (P34HB) blend fibers low P34HB (≤ 8 is successfully fabricated excellent spinnability. The incorporation contributes substantially...