A5088672406- Bacteriophages and microbial interactions
- Proteins in Food Systems
- Mycotoxins in Agriculture and Food
- Enzyme Production and Characterization
- Biopolymer Synthesis and Applications
- Bacterial biofilms and quorum sensing
- Protein Hydrolysis and Bioactive Peptides
- Food composition and properties
- Fungal Biology and Applications
- Probiotics and Fermented Foods
- Protein purification and stability
- Carbon and Quantum Dots Applications
- Polyamine Metabolism and Applications
- Phytochemicals and Antioxidant Activities
- Essential Oils and Antimicrobial Activity
- Electrochemical sensors and biosensors
- Antimicrobial agents and applications
- Antimicrobial Peptides and Activities
- Blood properties and coagulation
- Biofuel production and bioconversion
- Electrochemical Analysis and Applications
- Microbial infections and disease research
- Bee Products Chemical Analysis
- Magnetic and Electromagnetic Effects
- Bacterial Genetics and Biotechnology
Yangzhou University
2016-2025
Jiangnan University
2009-2023
State Key Laboratory of Food Science and Technology
2009-2023
Kyiv City Clinical Oncology Center
2019
State Council of the People's Republic of China
2018
In this study, the thermostability of an acid-resistant GH11 xylanase (xynA) from Aspergillus niger AG11 was enhanced through systematic modification its four highly flexible regions (HFRs) predicted using MD simulations. Among them, HFR I (residues 92-100) and II 121-130) were modified by iterative saturation mutagenesis (ISM), yielding mutants G92F/G97S/G100K T121V/A124P/I126V/T129L/A130N, respectively. For III, N-(residues 1-37) C-termini 179-188) were, respectively, substituted with...
Background: More than one-fifth of the world’s population consumes Chinese cuisines regularly, but no evidence-based healthy diets fitting food culture are available for implementation. Methods: A multicenter, patient- and outcome assessor–blind, randomized feeding trial was conducted among 265 participants with 130 to 159 mm Hg baseline systolic blood pressure (SBP) 4 major (Shangdong, Huaiyang, Cantonese, Szechuan). After a 7-day run-in period on control diet matching usual local diets,...
Streptomyces mobaraenesis transglutaminase can catalyze the cross-linking of proteins, which has been widely used in food processing. In this study, we rationally modified flexible regions to further improve thermostability FRAPD-TGm2 (S2P-S23V-Y24N-E28T-S199A-A265P-A287P-K294L), a stable mutant constructed our previous study. First, five were identified by molecular dynamics simulations at 330 and 360 K. Second, script based on Rosetta Cartesian_ddg was developed for virtual saturation...
Streptomyces mobaraenesis transglutaminase has been widely used in food processing. We here significantly improved the catalytic properties of S2P-S23V-Y24N-S199A-K294L (TGm1), a highly stabilized variant transglutaminase. First, virtual proline scan was performed based on folding free energy changes to obtain TGm1 variants with enhanced thermostability. Second, residues within 15 Å Cys64 enzyme-substrate complex were subjected saturation mutagenesis generate reduced binding and increased...
Brocade orange peels (BOPs), typically treated as discardable residue, contain large amounts of bioactive phenols that can serve ingredients for value-added products with health-promoting effects. Given this, this study developed a response surface model to optimize the conditions ultrasound-assisted extraction (UAE). The total phenol and flavonoid contents (TPC, TFC), profiles, antioxidant activities peel extracts free, esterified, glycosylated-bound (FPs, EBPs, GBPs) served metrics...
The present study investigated the effects of ultrasonic treatment combined with insoluble dietary fiber (IDF) from oat on gelation and structural characteristics myofibrillar protein (MP). Ultrasonic IDF promoted deployment MP chain significantly increased (P < 0.05) solubility MP. samples treated 120 W power (U120 + IDF) formed a more continuous dense gel microstructure, resulting water-holding capacity (WHC) reached maximun values 0.88 g/g. Moreover, compared U80 U120 groups, by 80 (U80...