A5100329957- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Aluminum toxicity and tolerance in plants and animals
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Heavy metals in environment
- Environmental Toxicology and Ecotoxicology
- Plant responses to water stress
- Trace Elements in Health
- Regional Economic and Spatial Analysis
- Reproductive biology and impacts on aquatic species
- Viral gastroenteritis research and epidemiology
- Arsenic contamination and mitigation
- Endoplasmic Reticulum Stress and Disease
- Polysaccharides and Plant Cell Walls
- Agricultural Engineering and Mechanization
- Cancer-related molecular mechanisms research
- Plant-Microbe Interactions and Immunity
- Marine Bivalve and Aquaculture Studies
- Plant Molecular Biology Research
- Physiological and biochemical adaptations
- Animal Virus Infections Studies
- Plant Gene Expression Analysis
- Soil Mechanics and Vehicle Dynamics
- Corrosion Behavior and Inhibition
Chinese Academy of Fishery Sciences
2021-2024
Nanjing Agricultural University
2015-2024
Case Western Reserve University
2024
Nanjing Tech University
2021-2023
Ministry of Agriculture and Rural Affairs
2023
Huazhong Agricultural University
2023
Shanghai University
2023
Virginia Tech
2022
Tianjin Medical University
2022
Wellcome Sanger Institute
2022
Metal homeostasis is critical for plant growth, development and adaptation to environmental stresses largely governed by a variety of metal transporters. The ZIP (Zn-regulated transporter, Iron-regulated transporter-like Protein) family proteins belong the integral membrane transporters responsible uptake allocation essential non-essential metals. However, whether members mediate efflux its regulatory mechanism remains unknown.In this report, we provided evidence that OsZIP1 metal-detoxified...
Abstract Nitrogen (N) and carbon (C) are essential elements for plant growth crop yield. Thus, improved N C utilisation contributes to agricultural productivity reduces the need fertilisation. In present study, we find that overexpression of a single rice gene, Oryza sativa plasma membrane (PM) H + -ATPase 1 ( OSA1 ), facilitates ammonium absorption assimilation in roots enhanced light-induced stomatal opening with higher photosynthesis rate leaves. As result, plants causes 33% increase...
The preference of paddy rice for NH(4)(+) rather than NO(3)(-) is associated with its tolerance to low pH since a rhizosphere acidification occurs during absorption. However, the adaptation root has not been fully elucidated. This study investigated acclimation plasma membrane H(+)-ATPase pH. Rice seedlings were grown either or NO(3)(-). For both nitrogen forms, value nutrient solutions was gradually adjusted 6.5 3.0. After 4 d cultivation, hydrolytic activity, V(max), K(m), H(+)-pumping...
To gain a better understanding of salt stress responses in plants, we used proteomic approach to investigate changes rice (Oryza sativa) root plasma-membrane-associated proteins following treatment with 150 mmol/L NaCl. With or without 48 h treatment, plasma membrane fractions from tip cells salt-sensitive cultivar, Wuyunjing 8, were purified by PEG aqueous two-phase partitioning, and separated IEF/SDS-PAGE using an optimized rehydration buffer. Comparative analysis three independent...
Abstract Chloroplast biogenesis has been well documented in higher plants, yet the complex methods used to regulate chloroplast activity under fluctuating environmental conditions are not understood. In rice (Oryza sativa), CYTOKININ-RESPONSIVE GATA TRANSCRIPTION FACTOR1 (Cga1) shows increased expression following light, nitrogen, and cytokinin treatments, while darkness gibberellin reduce expression. Strong overexpression of Cga1 produces dark green, semidwarf plants with reduced tillering,...
To cope with manganese (Mn) deficiency, plants have evolved an efficient transport system to uptake and redistribute Mn. However, the underlying molecular mechanisms remain be demonstrated. We carried out a forward genetic screen in root high-affinity Mn transporter nramp1 mutant background Arabidopsis thaliana identified uncharacterized NRAMP2. investigated effect of nramp2 mutation on growth reactive oxygen species (ROS) accumulation we also examined NRAMP2 expression pattern, subcellular...
Natural resistance-associated macrophage proteins (NRAMPs) have been shown to transport a wide range of divalent metal ions, such as manganese (Mn), cadmium (Cd), and Iron (Fe). is an essential micronutrient for plants Fe deficiency can lead chlorosis or decreased biomass. AtNRAMP6 has demonstrated the capability Cd, but its physiological function currently unclear. This study demonstrates that localized Golgi/trans-Golgi network plays important role in intracellular homeostasis flowering...
Protein homeostasis (proteostasis) deficiency is an important contributing factor to neurological and metabolic diseases. However, how the proteostasis network orchestrates folding assembly of multi-subunit membrane proteins poorly understood. Previous proteomics studies identified Hsp47 (Gene: SERPINH1 ), a heat shock protein in endoplasmic reticulum lumen, as most enriched interacting chaperone for gamma-aminobutyric acid type A (GABA ) receptors. Here, we show that enhances functional...
Abstract Vacuolar storage of iron (Fe) is important for Fe homeostasis in plants. When sufficient, excess could be stored vacuoles remobilization the case deficiency. Although mechanism from critical crop development under low stress, transporters that mediate vacuolar translocation into cytosol rice remains unknown. Here, we showed high Fe2+ concentrations, Δccc1 yeast mutant transformed with natural resistance-associated macrophage protein 2 gene (OsNRAMP2) became more sensitive to...
Porcine reproductive and respiratory syndrome, caused by porcine syndrome virus (PRRSV), is a panzootic disease that one of the most economically costly diseases to swine industry. A key aspect PRRSV virulence suppresses innate immune response induces persistent infection, although underlying mechanisms are not well understood. The dendritic cell (DC) marker CD83 belongs immunoglobulin superfamily associated with DC activation immunosuppression T proliferation when expressed as soluble...
The extensive use of carbamate pesticides has led to a range environmental and health problems, such as surface groundwater contamination, endocrine disorders in organisms. In this study, we focused on examining the effects toxic exposure pesticide methomyl hatching, morphology, immunity developmental gene expression levels zebrafish embryos. Four concentrations (0, 2, 20, 200 μg/L) were administered embryos for period 96 h. study found that accelerated hatching process embryos, with...
Nrat1 is a member of the natural resistance-associated macrophage protein (Nramp) family metal ion transporters in all organisms. Different from other Nramp members capable transporting divalent metals, specifically transports trivalent aluminum (Al) ion. However, molecular mechanism underlying Al transport selectivity remains unknown. Here, we performed structure-function analyses and to gain insights into determinants selectivity. A phylogenetic analysis showed that plant could be divided...
In plants, abscisic acid-, stress-, and ripening-induced (ASR) proteins have been shown to impart tolerance multiple abiotic stresses such as drought salinity. However, their roles in metal stress are poorly understood. To screen plant Cd-tolerance genes, the yeast-based gene hunting method which aimed colonies from maize leaf cDNA library hosted yeast was carried out. Here, ZmASR1 identified be putative Cd-tolerant through this survival screening strategy. silico analysis of functional...
It has been known that the process of leaf senescence is accompanied by programmed cell death (PCD) and previous study indicated dark-induced in detached leaves from rice led to release cytochrome f (Cyt f) chloroplast into cytoplasm. In this study, effects Cyt on PCD were studied both vitro vivo. a cell-free system, purified activated caspase-3-like protease endonuclease OsNuc37 induced DNA fragmentation. Furthermore, f-induced activity could be inhibited MG132, which suggests was...
Arabidopsis thaliana natural resistance-associated macrophage protein 3 (AtNRAMP3) is involved in the transport of cadmium (Cd), iron (Fe), and manganese (Mn). Here, we present a structure-function analysis AtNRAMP3 based on site-directed mutagenesis metal toxicity growth assays involving yeast mutants, combined with three-dimensional (3D) structure modeling crystal Eremococcus coleocola NRAMP family transporter, EcoDMT. We demonstrated that two conservative sites, D72 N75, are essential for...