A5024632021- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Plant Reproductive Biology
- Plant Pathogenic Bacteria Studies
- SARS-CoV-2 and COVID-19 Research
- Plant Stress Responses and Tolerance
- Viral gastroenteritis research and epidemiology
- Legume Nitrogen Fixing Symbiosis
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Parasitism and Resistance
- Plant nutrient uptake and metabolism
- Plant and animal studies
- Birth, Development, and Health
- Sexual Differentiation and Disorders
- Photosynthetic Processes and Mechanisms
- Testicular diseases and treatments
- Gestational Diabetes Research and Management
- Biochemical and Molecular Research
- 14-3-3 protein interactions
- Adipose Tissue and Metabolism
- Aluminum toxicity and tolerance in plants and animals
- Gut microbiota and health
- Endoplasmic Reticulum Stress and Disease
- Epigenetics and DNA Methylation
- Sperm and Testicular Function
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2018-2024
Guangxi University
2017-2024
Hudson Institute
2024
Monash University
2020-2024
Hudson Institute of Medical Research
2020-2024
Ministry of Education of the People's Republic of China
2018-2019
Zhejiang University
2018-2019
Tsinghua University
2011-2016
Lanzhou University
2015
Baker Heart and Diabetes Institute
2009
The COVID-19 RNA-synthesizing machine Many in the scientific community have mobilized to understand virus that is causing global coronavirus disease 2019 (COVID-19) pandemic. Gao et al. focused on a complex plays key role replication and transcription cycle of virus. They used cryo–electron microscopy determine 2.9-angstrom-resolution structure RNA-dependent RNA polymerase nsp12, which catalyzes synthesis viral RNA, with two cofactors, nsp7 nsp8. nsp12 target for nucleotide analog antiviral...
Abstract To date, an effective therapeutic treatment that confers strong attenuation toward coronaviruses (CoVs) remains elusive. Of all the potential drug targets, helicase of CoVs is considered to be one most important. Here, we first present structure full-length Nsp13 SARS-CoV (SARS-Nsp13) and investigate structural coordination its five domains how these contribute translocation unwinding activity. A model proposed for Upf1-like members according three different conditions in solution...
The lethally pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV) and the severe acute (SARS-CoV) pose serious threats to humans. Endoribonuclease Nsp15 encoded by plays an important role in viral infection pathogenesis. This study determines structure of MERS-CoV demonstrates how catalytic activity this protein is potentially mediated, thereby providing structural functional evidence for developing antiviral drugs. We also hypothesize that primase-like Nsp8 Nsp7/Nsp8 complex...
Abstract Plants have evolved a lignin-based Casparian strip (CS) in roots that restricts passive diffusion of mineral elements from the soil to stele. However, molecular mechanisms underlying CS formation rice (Oryza sativa), which contains at both exodermis and endodermis, are poorly understood. Here, we demonstrate endodermis is redundantly regulated by three MYELOBLASTOSIS (MYB) transcription factors, OsMYB36a, OsMYB36b, OsMYB36c, highly expressed root tips. Knockout all genes resulted...
Abstract A novel coronavirus (2019-nCoV) outbreak has caused a global pandemic resulting in tens of thousands infections and deaths worldwide. The RNA-dependent RNA polymerase (RdRp, also named nsp12), which catalyzes the synthesis viral RNA, is key component coronaviral replication/transcription machinery appears to be primary target for antiviral drug, remdesivir. Here we report cryo-EM structure 2019-nCoV full-length nsp12 complex with cofactors nsp7 nsp8 at resolution 2.9-Å. Additional...
Nicotinamide phosphoribosyltransferase (NAMPT) plays an important role in the biosynthesis of nicotinamide adenine dinucleotide (NAD
The existing reports about intergenerational or transgenerational effects of intrauterine hyperglycemia have included both and postnatal metabolic exposure factors, while the impact per se has not been assessed alone. A number studies suggest DNA methylation reprogramming gametes plays a crucial role in inheritance, but it is unclear when how patterns are altered exposed to hyperglycemia. In this study, we selected nondiabetic F1- F2-gestational diabetes mellitus (GDM) male mice as founders...
Objective This study aimed to determine the effects of diet‐induced paternal obesity on cognitive function in mice offspring. Methods Male (F0) were randomized receive either a control diet (10 kcal% fat) or high‐fat (HFD; 60 for 10 weeks before being mated with normal females generate F1 offspring F2 Behavioral tests used assess functions and Reduced representation bisulfite sequencing was explore mechanisms epigenetic inheritance. Results HFD‐induced resulted impairments offspring,...
The role of beta-adrenoceptors in heart disease remains controversial. Although beta-blockers ameliorate the progression disease, mechanism undefined. We investigated effect on cardiac hypertrophic growth using beta(1)- and beta(2)-adrenoreceptor knockout wild-type (WT) mice.Mice were subjected to aortic banding or sham surgery, their function was determined by echocardiography micromanometry.At 4 12 weeks after banding, left ventricle:body mass ratio increased 80-87% mice, but only 15%...
Accumulating evidence indicates that early and essential events for receptor-like kinase (RLK) function involve both autophosphorylation substrate phosphorylation. However, the structural biochemical basis these is largely unclear. Here, we used RLK FERONIA (FER) as a model crystallized its core domain (FER-KD) two FER-KD mutants (K565R, S525A) in complexes with ATP/ADP Mg2+ unphosphorylated state. Unphosphorylated was found to adopt an unexpected active conformation crystal structure....
The receptor-like kinase FLAGELLIN-SENSITIVE 2 (FLS2) functions as a bacterial flagellin receptor localized on the cell membrane of plants. In Arabidopsis, co-receptor BRI1-ASSOCIATED RECEPTOR KINASE 1 (BAK1) cooperates with FLS2 to detect epitope flg22, resulting in formation signaling complex that triggers plant defense responses. However, responsible for recognizing and flg22 rice remains undetermined, precise structural mechanism underlying FLS2-mediated signal activation transduction...
Nrat1 is a member of the natural resistance-associated macrophage protein (Nramp) family metal ion transporters in all organisms. Different from other Nramp members capable transporting divalent metals, specifically transports trivalent aluminum (Al) ion. However, molecular mechanism underlying Al transport selectivity remains unknown. Here, we performed structure-function analyses and to gain insights into determinants selectivity. A phylogenetic analysis showed that plant could be divided...
All known riboswitches use their aptamer to senese one metabolite signal and expression platform regulate gene expression. Here, we characterize a SAM-I riboswitch (SAM-IXcc) from the Xanthomonas campestris that regulates methionine synthesis via met operon. In vitro in vivo experiments show SAM-IXcc controls operon primarily at translational level response cellular S-adenosylmethionine (SAM) levels. Biochemical genetic data demonstrate not only can repress SAM binding but also sense bind...
The zinc uptake regulator (Zur) is a member of the Fur (ferric regulator) family transcriptional regulators that plays important roles in homeostasis and virulence bacteria. Upon perception, Zur binds to promoters responsive genes controls their transcription. However, mechanism underlying zinc-mediated activation remains unclear. Here we report 2.2-Å crystal structure apo from phytopathogen Xanthomonas campestris pv. (XcZur), which reveals molecular XcZur exists closed inactive state before...
Abstract Background The N-terminal regulatory element (NRE) of Receptor-like kinases (RLKs), consisting the juxtamembrane segment in receptor (RKs) and extension RLCKs, is a crucial component that regulates activities these proteins. However, features functions NRE have remained largely unexplored. Herein, we comprehensively analyze 510,233 sequences RLKs from 528 plant species, using information theory data mining techniques to unravel their common characteristics diversity. We also use...
Structured Abstract Objective To investigate the role of a potential SOX9 target gene, Tyro3 , along with its family members, Axl and Mertk (TAM family) in mouse testis development. Design Experimental laboratory study. Setting Research institute units. Subject(s) Embryonic day (E)11.5 Swiss gonads for ex vivo gonad culture; knockout embryos. Intervention(s) E11.5 were cultured hanging droplets 30 µL DMEM medium supplemented 10% FBS 1% antibiotic-antimycotic. A pair treated 20 μM BMS-777607...
Summary The RNA chaperone, Hfq, is known to play extensive roles in bacterial growth and development. More recently, it has been shown be required for virulence many human animal pathogens. Despite these studies little about the role Hfq plays phytopathogenic bacteria. In this study, we show full of crucifer black rot pathogen Xanthomonas campestris pv. ( Xcc ). We demonstrate that an hfq deletion strain highly attenuated Chinese radish shows a severe defect production factors including...
Abstract In plants, clade A type 2C protein phosphatases (PP2CAs) have emerged as major players in abscisic acid (ABA)‐regulated stress responses by inhibiting kinase activity. However, how different internal and external environmental signals modulate the activity of PP2CAs are not well known. The transmembrane (TMK) 4 (TMK4), one member a previously identified receptor subfamily on plasma membrane that plays vital roles plant cell growth, directly interacts with (ABA‐Insensitive 2, ABI2)....
Brown adipose tissue (BAT) is an exclusive of nonshivering thermogenesis. It fueled by lipids and glucose involved in energy metabolic homeostasis. Intrauterine exposure to hyperglycemia during gestational diabetes mellitus may result abnormal fetal development phenotypes adulthood. However, whether intrauterine influences the BAT unknown. In this study, mouse embryos were exposed environment injecting streptozocin into pregnant mice at 1 d post coitum (dpc). The structure was examined...