Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation and is overexpressed in various human malignancies. However, its role head neck squamous cell carcinoma (HNSCC) remains unclear. Here we demonstrate that C1GALT1 expression upregulated HNSCC tumors associated with adverse clinicopathologic features. Moreover, high predicts poor disease-free overall survivals. overexpression enhances viability, migration, invasion, which can be reversed by...

Abstract C1GALT1 controls the crucial step of GalNAc-type O-glycosylation and is associated with both physiologic pathologic conditions, including cancers. EPH receptors comprise largest family receptor tyrosine kinases (RTKs) modulate a diverse range developmental processes human diseases. However, role in signaling remains largely overlooked. Here, we showed that high expression gastric adenocarcinomas correlated adverse clinicopathologic features an independent prognostic factor for poor...

Mucins are heavily glycosylated proteins that play critical roles in the pathogenesis of tumour malignancies. Pancreatic ductal adenocarcinoma (PDAC) is characterised by aberrant expression mucins. However, role mucin (MUC) 20 PDAC remains unclear. usually surrounded a dense fibrotic stroma consisting an extracellular matrix and pancreatic stellate cells (PSCs). The creates nutrient-deprived, hypoxic, acidic microenvironment, promotes malignant behaviours cells. In this study,...

Abstract Pancreatic adenocarcinoma (PDAC) is a leading cause of cancer-related death. Altered glycosylation contributes to tumor progression and chemoresistance in many cancers. C1GALT1 the key enzyme controlling elongation GalNAc-type O-glycosylation. Here we showed that was overexpressed 85% (107/126) PDAC tumors compared with adjacent non-tumor tissues. High expression associated poor disease-free overall survival ( n = 99). knockdown using siRNA suppressed cell viability, migration,...

Hyperglycemia and hyperglycosuria, two primary characteristics of diabetes mellitus, may increase the risk cancer initiation, particularly for bladder cancer. The effectiveness metformin, a common antidiabetic agent, is determined by its ability to induce GDF15. However, mechanism GDF15 in relation glucose, which influences tumor microenvironment human bladder, not fully understood. This study explores potential roles response glucose bladder. High treatment (30 mM) enhanced phosphorylation...

Ovarian cancer is the most lethal of gynecologic malignancies. N-acetylgalactosaminyltransferase 6 (GALNT6), an enzyme that mediates initial step mucin type-O glycosylation, has been reported to regulate mammary carcinogenesis. However, expression and role GALNT6 in ovarian are still unclear. Here we showed high correlates with increased recurrence, lymph node metastasis, chemoresistance endometrioid clear cell carcinomas; higher levels significantly associated poorer patient survivals....

Abstract The glycoprotein CD44 is a key regulator of malignant behaviors in breast cancer cells. To date, hyaluronic acid (HA)-CD44 signaling pathway has been widely documented the context metastatic bone diseases. Core 1 β1,3-galactosyltransferase (C1GALT1) critical enzyme responsible for elongation O-glycosylation. Aberrant O-glycans recognized as hallmark cancers. However, effects C1GALT1 on and metastasis remain unclear. In this study, IHC analysis indicated that expression positively...

In endemic area, nasopharyngeal carcinoma (NPC) tumor cells harbor EBV latent infection and expresses viral antigens such as EBNA1, LMP1 LMP2. this study, we established a NPC-mimicry animal model assessed the therapeutic potential of vaccine.Animal models were by injection LMP1-expressing TC-1 in C57BL6/J mice subcutaneously or through tail veins. pcDNA3.1 empty vector LMP1/pcDNA3.1 vaccine was delivered helium-driven gene gun. Effectiveness evaluated measuring size numbers metastatic lung...

Functions of metallothionein 2A (MT2A) in bladder cancer have not been extensively explored even though metallothioneins are regarded as modulators several biological regulations including oxidation and cancerous development. We evaluated MT2A carcinoma cells terms the mechanisms regulation underlying functions. overexpression only downregulated endogenous ROS but also blocked induced by H2O2. used annexin V-FITC apoptosis assay to determine modulation H2O2-induced cell expression. Results...

GalNAc‐type O‐glycosylation and its initiating GalNAc transferases (GALNTs) play crucial roles in a wide range of cellular behaviors. Among 20 GALNT members, GALNT2 is consistently associated with poor survival patients colorectal cancer public databases. However, clinicopathological significance remains unclear. In this study, immunohistochemistry showed that was overexpressed tumors compared the adjacent nontumor tissues. overexpression patients. Forced expression promoted migration...

Neuroblastoma (NB) is a childhood tumor derived from the sympathoadrenal lineage of neural crest progenitor cells. Core 1 β1,3-galactosyltransferase (C1GALT1) controls crucial step GalNAc-type O-glycosylation, and its altered expression affects cancer behaviors. However, role C1GALT1 in NB tumors remains unclear. Our data showed that was significantly associated with differentiated histology, correlated TrkA expression, predicted good prognosis independently NB. Downregulation promotes...

The WNT1 inducible signaling pathway protein 1 (WISP1), a member of the connective tissue growth factor family, plays crucial role in several important cellular functions highly tissue-specific manner. Results RT-qPCR indicated that WISP1 expressed only cells human prostate fibroblasts, HPrF and WPMY-1, but not carcinoma vitro. Two major isoforms (WISP1v1 WISP1v2) were identified determined by RT-PCR immunoblot assays. knock-down blocked cell proliferation contraction, while treating...

The functions of activating transcription factor 3 (ATF3) within the human bladder remain unexplored. This study delves into expressions, functions, and regulatory mechanisms ATF3 in cancer. Gene expressions were determined by immunoblot, RT-qPCR, reporter assays. Assays Ki67, colony formation, Matrigel invasion, xenograft animal used to assess cell proliferation, tumorigenesis vitro vivo. Silico analysis from TCGA database examined correlations between GDF15 clinicopathologic features,...

Glucose can activate the carbohydrate response element binding protein (ChREBP) transcription factor to control gene expressions in metabolic pathways. The way of ChREBP involvement human prostate cancer development remains undetermined. This study examined interactions between fibroblasts and cells under influences ChREBP. Results showed that high glucose (30 mM) increased phosphorylation AKT at S473 AMP-activated kinase (AMPK) S485 fibroblast (HPrF) PC-3 cells. High enhanced expression...

The androgen-dependent or -independent pathways are regarded as primary therapeutic targets for the neoplasm of prostate. Mucosa-associated lymphoid tissue 1 (MALT1) acting a paracaspase in regulation nuclear factor κB (NF-κB) signal transduction plays central role inflammation and oncogenesis cancers. This study confirmed potential linkages between androgen NF-κB activation by inducing MALT1 receptor-full length (ARFL)-positive LNCaP 22Rv1 prostate cancer cells. Although did not stimulate...

<div>Abstract<p>The glycoprotein CD44 is a key regulator of malignant behaviors in breast cancer cells. To date, hyaluronic acid (HA)-CD44 signaling pathway has been widely documented the context metastatic bone diseases. Core 1 β1,3-galactosyltransferase (C1GALT1) critical enzyme responsible for elongation O-glycosylation. Aberrant O-glycans are recognized as hallmark cancers. However, effects C1GALT1 on and metastasis remain unclear. In this study, immunohistochemical analysis...

<p>C1GALT1 knockdown in breast cancer cells did not cause significant changes CD44 mRNA levels.</p>

<p>Effects of CD44 O-glycans on tumor sphere formation in breast cancer cells.</p>

<p>Construction of multiple mutations at O-glycosites in the stem region CD44.</p>

<p>Effects of CD44 O-glycans on tumor sphere formation in breast cancer cells.</p>

<p>C1GALT1 knockdown in breast cancer cells did not cause significant changes CD44 mRNA levels.</p>