Double antibody radioimmunoassays have been developed to measure gonadotropinreleasing hormone (Gn-RH). Thyrotropin-releasing hormone, melanocyte-stimulating inhibiting factor, various Gn-RH analogs and the components of serum per se did not appear influence assay. Serum containing endogenous or plus exogenous Gn-RH, crude hypothalamic extracts, analogues all produced inhibition curves which were parallel those obtained with synthetic standard. Exogenous could be quantitatively recovered...

Levels of luteinizing hormone (LH), estradiol-17 beta and progesterone were determined by specific radioimmunoassays in sera obtained from Beagle bitches during proestrus, estrus diestrus. Concentrations LH (expressed as NIH-LH-SI equivalents) 2.8 plus or minus 0.1 ng/ml 35.5 10.0 early 2.2 Peak levels estradiol-17beta (68.9 11.0 ng/ml) detected 24 hr prior to the peak, declined rapidly reached basal (17.8 6.3 five days following peak. 1.7 0.3 3.5 23.3 on day 5 after peak . Progesterone...

<title>Abstract</title> <bold>Introduction:</bold> Injections are frequently used in developing countries as a perceived potent medical treatment. However, unsafe injection practices, including the reuse of syringes without proper sterilization, can transmit infectious diseases like hepatitis B, C, and HIV. In Pakistan, average rate is 8.5 per person annually, with significant proportion deemed unnecessary. Cultural beliefs mutual reinforcement between healthcare providers patients...

Acute leukoencephalopathy with restricted diffusion (ALERD) is a distinct, severe clinical-radiological syndrome characterised by encephalopathy, seizures and in the subcortical white matter. ALERD one of infection-associated encephalopathy syndromes seen childhood having higher incidence neurological sequelae. We report three cases ALERD, all following prodrome fever gastroenteritis, progressing to varying outcomes. Case 1 was diagnosed as complicated dengue multiple organ dysfunction...

Blood samples were collected at 4-min intervals for 4 hr from 2 ewes on the day of estrus and castrated 90 120 days previously. Serum levels luteinizing hormone (LH) gonadotropin-releasing (Gn-RH) determined by double antibody radioimmunoassays. Samples during a portion preovulatory LH surge in each ewe with ranging <5 ng/ml to=300 ng/ml. Gn-RH ranged 15 to 96 pg/ml not significantly elevated when high. 2.7 13.3 while 119 368 4-hr sampling period. There was no apparent correlation between...

Indwelling cannulas were placed into a branch of the uterine vein and utero-ovarian 10 nonpregnant ewes only eight pregnant ewes. Blood samples collected chilled, heparinized tubes at 3-hr intervals from days 11 to 14 (three five cycling ewes) or 17 (five after estrus. Levels prostaglandin (PG) F2α determined by radioimmunoassay. No significant difference was noted in mean levels PGF2α between (9.1 ± .6 ng/ml, n=458) (8.1 .1 n=386) However, there more variation (P<.01) than This reflected...

A Doppler ultrasonic blood flow procedure was evaluated to determine if it could be adapted for use on vessels as small (I.D. ≅ 0.5 mm) the ovarian artery in sheep. When pumped through silastic tubing = 0.38 and 0.57 values calculated using were highly correlated (P<0.01) with known rates. second experiment conducted compare measuring ovaries of ewes uptake radioactive microspheres. Although not possible measure directly (ml/min) microsphere method, when cpm/g tissue compared ml/min...

A specific radioimmunoassay for bovine follicle-stimulating hormone (bFSH) has been developed using rabbit antiserum to ovine FSH and radioiodinated human FSH. Employing this radioimmunoassay, as little 3 ng of can be quantified. Inhibition curves obtained with serum, crude pituitary extract other partially purified preparations containing were parallel those the bFSH standard preparation. estimates by in varying amounts FSH, LH, TSH, GH prolactin good agreement values bioassay. Exogenous...

Metabolic clearance rates (MCR) were determined in 5 ewes by injection of a priming dose and constant infusion luteinizing hormone (LH)-125I, follicle-stimulating (FSH)-131I prolactin-125I for 120 min during the midluteal follicular phases estrous cycle on day estrus. Blood samples collected at 10-min intervals period to quantify serum levels gonadotropins. MCR (ml/min) was calculated as ratio immunoprecipitable radioiodinated infused (counts infused/min) (cpm/ml) after equilibrium obtained....

The success of development depicts economic growth. Nevertheless, it is pertinent to emphasize that a significant increase in growth may not eradicate poverty, unemployment, and health issues. prevalence poverty Palembang City 2021 was considerably elevated, amounting 11.34 percent, while the unemployment rate has exhibited an upward trend, reaching 10.11 percent. Additionally, there decline employment within labor market, accompanied by corresponding rise number individuals actively seeking...

Abstract Marine organic waste can be managed into compost products. Co-composting is a biological process to decompose two or more types of matter stable humus substance and expected overcome the disadvantages composting single matter. This study aims produce with better characteristics than marine singly addition other materials such as cow dung rice straw. The method used Barkley for 7 weeks. Parameters observed were pH, temperature, humidity, C/N, macronutrients, micronutrients, physical...

Abstract It is estimated that marine waste consist of 60% organic material. Therefore, must be handled using a composting process. The main determining factor in the process microorganisms associated with C/N ratio. this research was conducted to observe dynamics microbial populations during decomposition waste. method for calculating (bacteria and fungi) dilution colony counter Pour Platedilution technique (CFU/g). results showed population, both fungi bacteria, fluctuated from week 1 10...

Hypomagnesaemia in children with cystic fibrosis (CF) is under‐recognized. We report a child CF who developed significant hypomagnesaemia following intravenous (i.v.) treatment aminoglycosides for exacerbations of Pseudomonas aeruginosa infection. Three additional cases have also been observed. Investigations two patients revealed excessive renal loss magnesium. It postulated that tubular damage secondary to the cumulative effects repeated courses resulted hypomagnesaemia, and we suggest...

Fasting serum gastrin concentrations were determined by radioimmunoassay for 124 infants and children free of gastrointestinal disease. Values the entire group ranged from 1.1 to 167.9 pg/ml (mean, 32.3 pg/ml). Mean levels who fasted eight or more hours 22.5 ± 23.9 while mean four 42.4 33.9 59.2 49.6 pg/ml. The varied inversely with body surface area among same length time.

The effect of 17β-estradiol on the in vitro conversion pregnenolone-4-14C to progesterone by luteal slices mature ewes days 6 and 13 estrous cycle (first day estrus=day zero cycle) was studied. Luteal were incubated 3 ml Krebs Ringer bicarbonate with 0.3, 1.0 or 10 µg/ml 0.2 µCi for 1.5 hr. at 37 C under an atmosphere 95% O2-5% CO2. converted more labeled pregnenolone than (P<.05). Estradiol (10 µg/ml) inhibited both stages studied (P<.01). Stage estradiol concentration without significant...

Serum LH levels and mean corpus luteum (CL) weights were studied in groups of four ewes following a single injection 750 µg 17β-estradiol into each ewe on days 4 or 11 the estrous cycle (first day estrus = 0 cycle). Mean CL 2 after estradiol treatment compared with removed from same at laparotomy 6 13 previous control cycle. Injection increased weight, whereas dose reduced weight. Differences luteal due to not, however, significant.

A 2.5 year old girl with metachromatic leukodystrophy presented acute respiratory distress and was initially wrongly diagnosed pneumothorax. Barium meal showed bowel loops in the left hemithorax, which prompted surgical intervention; spontaneous rupture of diaphragm at surgery.

The effect of exogenous estradiol on luteal weights in unilaterally hysterectomized ewes was studied two experiments. Ewes were day 5 the estrous cycle (first estrus=day zero cycle). Estradiol (1 mg) injected intramuscularly each days 11 and 12 cycle. All autopsied 14 Injections significantly reduced both intact ewes. In unilaterally-hysterectomized estradiol-treated corpora lutea ovary adjacent to uterine horn smaller size than opposite ovary. These latter data suggest that corpus luteum...

Abstract Fires in peatland have numerous detrimental effects as regards degradation of the environment especially changes soil properties. The purpose this study is to ascertain how drying and rewetting peatlands affects physical chemical characteristics post-fire peat with two depths. This research was carried out Dusun XIII, Perbangunan Village, Sei Kepayang, Asahan Regency. factor re-wetting past fire peatland, namely: unburnt wet conditions (P-nw), post-burnt dry (P-bd), (P-bw) within 2...

Fahr’s syndrome is a rare neurodegenerative disease characterised by symmetric calcifications in basal ganglia, cerebellum and cerebral cortex, secondary to genetic, infectious autoimmune aetiologies. It has wide range of clinical presentations including neurological psychiatric symptoms leading cognitive impairment behavioural changes. In this case report, an adolescent boy with unique sequence different manifestations APS-1 described who eventually developed hypoparathyroidism. The child...