A5093432622- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- Chemical Synthesis and Analysis
- Cancer Genomics and Diagnostics
- Chromatin Remodeling and Cancer
- Single-cell and spatial transcriptomics
Northwestern University
2025
University at Albany, State University of New York
2023-2024
In single cells, variably sized nanoscale chromatin structures are observed, but it is unknown whether these form a cohesive framework that regulates RNA transcription. Here, we demonstrate the human genome an emergent, self-assembling, reinforcement learning system. Conformationally defined heterogeneous, nanoscopic packing domains by interplay of transcription, nucleosome remodeling, and loop extrusion. We show not topologically associated domains. Instead, exist across structure-function...
A central question in biology is how RNA sequence changes influence dynamic conformational during cotranscriptional folding. Here we investigated this through the study of transcriptional fluoride riboswitches, non-coding RNAs that sense anion coordinated folding and rearrangement a pseudoknotted aptamer domain downstream intrinsic terminator expression platform. Using combination Escherichia coli polymerase vitro transcription cellular gene assays, characterized function mesophilic...
Cancer cells exhibit a remarkable resilience to cytotoxic stress, often adapting through transcriptional changes linked alterations in chromatin structure. In several types of cancer, these adaptations involve epigenetic modifications and restructuring topologically associating domains (TADs). However, the underlying principles by which architecture facilitates such adaptability across different cancers remain poorly understood. To investigate role this process, we developed physics-based...
ABSTRACT A central question in biology is how RNA sequence changes influence dynamic conformational during cotranscriptional folding. Here we investigated this through the study of transcriptional fluoride riboswitches, non-coding RNAs that sense anion coordinated folding and rearrangement a pseudoknotted aptamer domain downstream intrinsic terminator expression platform. Using combination E. coli polymerase vitro transcription cellular gene assays, characterized function mesophilic...