A5070353456- Cancer Genomics and Diagnostics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Hepatitis B Virus Studies
- Molecular Biology Techniques and Applications
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Hepatocellular Carcinoma Treatment and Prognosis
- Renal cell carcinoma treatment
- Metal complexes synthesis and properties
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Advanced biosensing and bioanalysis techniques
- Pancreatic and Hepatic Oncology Research
- Cellular Mechanics and Interactions
- Genomics and Phylogenetic Studies
- Chromosomal and Genetic Variations
- Magnetism in coordination complexes
- Infectious Diseases and Tuberculosis
- Cancer-related molecular mechanisms research
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- Hepatitis C virus research
Government Medical College and Hospital
2022-2024
Central Council for Research in Homoeopathy
2020-2024
Northwestern University
2021-2024
University of Illinois Urbana-Champaign
2016-2024
Sri Ramachandra Institute of Higher Education and Research
2024
Sana Biotechnology (United States)
2023
Ambedkar University Delhi
2022
All India Institute of Medical Sciences Bhopal
2022
Healing Institute
2022
JBS Science (United States)
2014-2021
Abstract Genetic engineering of allogeneic cell therapeutics that fully prevents rejection by a recipient’s immune system would abolish the requirement for immunosuppressive drugs or encapsulation and support large-scale manufacturing off-the-shelf products. Previously, we generated mouse human hypoimmune pluripotent (HIP) stem cells depleting HLA class I II molecules overexpressing CD47 ( B2M −/− CIITA + ). To determine whether this strategy is successful in non-human primates, engineered...
Abstract Genome editing critically relies on selective recognition of target sites. However, despite recent progress, the underlying search mechanism genome-editing proteins is not fully understood in context cellular chromatin environments. Here, we use single-molecule imaging live cells to directly study behavior CRISPR/Cas9 and TALEN. Our reveals that Cas9 less efficient heterochromatin than TALEN because becomes encumbered by local searches non-specific sites these regions. We find up a...
nano-ChIA combines multiple nanoimaging modalities to uncover 4D chromatin organization and its relationship molecular function.
B-type lamins are critical nuclear envelope proteins that interact with the three-dimensional genomic architecture. However, identifying direct roles of B-lamins on dynamic genome organization has been challenging as their joint depletion severely impacts cell viability. To overcome this, we engineered mammalian cells to rapidly and completely degrade endogenous using Auxin-inducible degron technology.
Palladium supported on amorphous silica, mercapto-functionalized amine functionalized and zeolite Y has been studied as a catalyst in the Heck reaction of iodobenzene with butyl acrylate presence triethylamine base dimethylformamide solvent. Trapping soluble Pd poly(4-vinylpyridine), hot filtration tests during batchwise reaction, effluents from fixed bed continuous reactor support conclusion that leached is active phase for all catalysts tested. Two different paths leaching depend chemical...
Hepatitis B virus (HBV) is a hepatotropic causing hepatitis, cirrhosis and hepatocellular carcinoma (HCC). The methylation status of the HBV DNA in its different forms can potentially provide insight into pathogenesis HBV-related liver diseases, including HCC, however this unclear. goal study to obtain comprehensive profiles three putative CpG islands infected livers, with respect disease progression. extent these was first assessed using bisulfite PCR sequencing small set tissue samples,...
Hepatoma Research is an open access journal and focuses on all topics related to hepatoma. The following articles are especially welcome: pathogenesis, clinical examination early diagnosis of hepatoma, complications their preventions treatments, etc.
Endothelial cell (EC) activation plays a key role in vascular inflammation, thrombosis, and angiogenesis. Allograft inflammatory factor-1 (AIF-1) is cytoplasmic, calcium-binding, inflammation-responsive scaffold protein that has been implicated the regulation of inflammation. The expression function AIF-1 EC uncharacterized, purpose this study was to characterize ECs. colocalized with CD31-positive ECs neointima inflamed human arteries but not normal arteries. detected at low levels...
We demonstrated previously that urine contains low-molecular-weight (LMW) (<300 bp), circulation-derived DNA can be used to detect cancer-specific mutations if a tumor is present. The goal of this study was develop an assay the colorectal cancer (CRC)–associated, circulation-derived, epigenetic marker hypermethylated vimentin gene (mVIM) in patients with CRC. An artificial 18-nucleotide sequence tagged at 5′ end primers first PCR cycle increase amplicon size, which then integrated into...
Objective— To characterize the expression and function of interleukin (IL) 19, a recently described T-helper 2 anti-inflammatory IL, on endothelial cell (EC) pathophysiological features. Methods Results— The effects ILs EC activation development angiogenesis are uncharacterized. We demonstrate by immunohistochemistry immunoblot that IL-19 is expressed in inflamed, but not normal, human coronary endothelium can be induced cultured ECs serum basic fibroblast growth factor. mitogenic...
Hypermethylation of the glutathione S-transferase π 1 (GSTP1) gene promoter region has been reported to be a potential biomarker distinguish hepatocellular carcinoma (HCC) from other liver diseases. However, reports regarding how specific marker it is have ranged 100% 0%. We hypothesized that, large extent, variation specificity depends on location CpG sites analyzed. To test this hypothesis, we compared methylation status GSTP1 DNA isolated HCC, cirrhosis, hepatitis, and normal tissues by...
The concerted action of multiple genes in a time-dependent manner controls complex cellular phenotypes, yet the temporal regulation gene expressions is restricted on single-gene level, which limits our ability to control higher-order networks and understand consequences multiplex genetic perturbations. Here we developed system for genes. This combines simplicity CRISPR/Cas9 activators orthogonal targeting orthogonality chemically induced dimerizing (CID) proteins activator function. In human...
In the current study, four novel mononuclear Cu(<sc>ii</sc>) complexes with terpyridine (<bold>L</bold>) and different co-ligands (phen, bipy, imd) were synthesized characterized in detail, where<bold>L</bold>is 4′-(3,4,5-trimethoxyphenyl)-2,2′:6′,2′′-terpyridine.
As imaging techniques rapidly evolve to probe nanoscale genome organization at higher resolution, it is critical consider how the reagents and procedures involved in sample preparation affect chromatin relevant length scales. Here, we investigate effects of fluorescent labeling DNA sequences within using gold standard technique three-dimensional fluorescence situ hybridization (3D FISH). The chemical 3D FISH protocol, specifically formamide, cause significant alterations sub-200 nm (sub-Mbp)...
Possible modes of deactivation Jacobsen's Co-salen catalyst during the hydrolytic kinetic resolution (HKR) epichlorohydrin were explored by UV-vis spectroscopy, X-ray absorption and electrospray ionization mass spectrometry, combined with recycling studies. Although an active Co(III)-salen deactivated substantially after multiple cycles without regeneration, maintained its +3 oxidation state throughout runs. Thus, HKR was not result Co reduction. The spectrum a material showed that...
Hypermethylation of the promoter tumor suppressor gene, adenomatous polyposis coli (APC), occurs in various malignancies, including hepatocellular carcinoma (HCC). However, reports on specificity methylation APC gene for HCC have varied. To gain insight into how these variations occur, bisulfite PCR sequencing was performed to analyze status both sense and antisense strands samples tissue, matched adjacent non-HCC liver hepatitis, cirrhosis, normal tissues. DNA derived from fetal 12...
Aberrant methylation of the promoter, P2, and first exon, E1, regions tumor suppressor gene RASSF1A, have been associated with hepatocellular carcinoma (HCC), albeit poor specificity. This study analyzed profiles P1, P2 E1 to identify region which most specifically corresponds HCC evaluate potential this methylated as a biomarker in urine for screening.Bisulfite DNA sequencing quantitative methylation-specific polymerase chain reaction assays were performed compare 56 CpG sites isolated from...