A5000911849- Genomics and Chromatin Dynamics
- Gene expression and cancer classification
- RNA Research and Splicing
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Cancer, Lipids, and Metabolism
- Plant Molecular Biology Research
- Cancer Genomics and Diagnostics
- RNA modifications and cancer
- Enzyme Production and Characterization
- Enzyme Structure and Function
- CRISPR and Genetic Engineering
- Chromatin Remodeling and Cancer
- Estrogen and related hormone effects
- Advanced biosensing and bioanalysis techniques
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Steroid Chemistry and Biochemistry
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
Northwestern University
2017-2023
Western Illinois University
2016-2020
nano-ChIA combines multiple nanoimaging modalities to uncover 4D chromatin organization and its relationship molecular function.
The CPMC model demonstrates the regulatory role of chromatin’s physical structure on transcription and phenotypic plasticity.
Abstract Improved understanding of local breast biology that favors the development estrogen receptor negative (ER−) cancer (BC) would foster better prevention strategies. We have previously shown overexpression specific lipid metabolism genes is associated with ER− BC. now report results exposure MCF-10A and MCF-12A cells, mammary organoids to representative medium- long-chain polyunsaturated fatty acids. This caused a dynamic profound change in gene expression, accompanied by changes...
Nocardia cholesterolicum NRRL 5767 is well-known for its ability to convert oleic acid 10-hydroxystearic (~88%, w/w) and 10-ketostearic (~11%, w/w). Conversion of then has been proposed be catalyzed by oleate hydratase secondary alcohol dehydrogenase, respectively. Hydroxy fatty acids are value-added with many industrial applications. The objective this study was improve the NRRL5767 strain CRISPR/Cas9 genome editing technology knockout dehydrogenase gene, thus blocking conversion acid....
Abstract In eukaryotic cells, chromatin structure is linked to transcription processes through the regulation of genome organization. Extending across multiple length-scales - from nucleosome higher-order three-dimensional structures a dynamic system which evolves throughout lifetime cell. However, no individual technique can fully elucidate behavior organization and its relation molecular function at all length- timescales both single-cell cell population level. Herein, we present...
Cancer cells exhibit a remarkable resilience to cytotoxic stress, often adapting through transcriptional changes linked alterations in chromatin structure. In several types of cancer, these adaptations involve epigenetic modifications and restructuring topologically associating domains (TADs). However, the underlying principles by which architecture facilitates such adaptability across different cancers remain poorly understood. To investigate role this process, we developed physics-based...
The production of household cooking oils in an industrial setting yields soapstock as a byproduct during the refining process. Soapstock contains significant amount oleic acid and due to soapstocks very low price, it serves cheap source acid. Oleate hydratase (Ohase) converts industrially useful product 10(R)‐hydroxystearic (10‐HSA), regioselective manner. Classically, 10‐HSA is utilized thicken grease, but has potential be used plastics, lubricants other various relevant materials. Barring...
Abstract Background: There are no targeted pharmacologic interventions currently available for the prevention of hormone receptor negative breast cancer. Primary with endocrine agents decreases risk ER positive disease effect against (ER-) disease. Thus, there is a compelling need to identify women at high ER-negative cancer and uncover molecular mechanisms involved in its genesis. Our recent observation that set lipid metabolism (LiMe) genes over-expressed contralateral unaffected breasts...
Norcardia cholesterolicum NRRL 5767 ( N. 5767) is a well‐known bacterium capable of transforming 95% added oleic acid to more valuable product, 10‐hydroxystearic (10‐HSA) (Appl Microbiol Biotechnol 32, 299–304, 1989). This reaction catalyzed by oleate hydratase. However, small amount the 10‐HSA subsequently converted 10‐ketostearic (10‐KSA) secondary alcohol dehydrogenase (2º‐ADH). Since than 10‐KSA in industry; considered side product which complicates downstream separation and purification...
Nocardia Cholesterolicum NRRL 5767 is well‐known for the ability to transform about 95% of added oleic acid value‐added product, namely 10‐hydroxystearic (10‐HSA). Oleate hydratase enzyme catalyzes this conversion. The objective research was clone, overexpress, and characterize oleate hydratase. genome contains two annotated Here, we report cloning an gene from N. cholestericum genomic DNA by PCR. inserted into pET28a. Expression functional recombinant achieved IPTG induction. produce large...