A5025852508- Advanced Fluorescence Microscopy Techniques
- Genomics and Chromatin Dynamics
- Advanced Electron Microscopy Techniques and Applications
- Cell Image Analysis Techniques
- Near-Field Optical Microscopy
- Spectroscopy Techniques in Biomedical and Chemical Research
- Dementia and Cognitive Impairment Research
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Advanced Biosensing Techniques and Applications
- Adipose Tissue and Metabolism
- Alzheimer's disease research and treatments
- Machine Learning in Healthcare
- Cardiovascular Function and Risk Factors
- Soil Moisture and Remote Sensing
- Cellular Mechanics and Interactions
- Advanced biosensing and bioanalysis techniques
- Gold and Silver Nanoparticles Synthesis and Applications
- Genetics, Bioinformatics, and Biomedical Research
- Brain Tumor Detection and Classification
- Soil Geostatistics and Mapping
- Advanced Chemical Sensor Technologies
- Synthesis of Tetrazole Derivatives
- AI in cancer detection
- Nanoparticle-Based Drug Delivery
Northwestern University
2016-2023
AbbVie (United States)
2021-2023
University of Illinois Chicago
2013-2016
Diabetes mellitus (DM) is associated with mitochondrial oxidative stress. We have shown that myocardial stress leads to diastolic dysfunction in a hypertensive mouse model. Therefore, we hypothesized diabetes could cause through and mitochondria-targeted antioxidant (MitoTEMPO) prevent diabetic C57BL/6J mice were fed either 60 kcal % fat diet (high-fat [HFD]) or normal chow (control) for 8 weeks without concurrent MitoTEMPO administration, followed by vivo assessment of function ex studies....
nano-ChIA combines multiple nanoimaging modalities to uncover 4D chromatin organization and its relationship molecular function.
Significance Chromatin is one of the most critical structures within cell because it houses genetic information. Its structure well understood at nucleosomal (<20-nm) and chromosomal (>200-nm) levels; however, due to lack quantitative imaging modalities study this organization, little known about higher-order between these length scales in live cells. We present a label-free technique, live-cell partial-wave spectroscopic (PWS) microscopy, with sensitivity 20 200 nm that can quantify...
Results have historically shown a broad plasticity in the origin of tumors and their functions, with significant heterogeneity observed both morphologies functional capabilities. Largely unknown, however, are mechanisms by which these variations occur how events influence tumor formation behavior. Contemporary views on focus mainly role particular sets driver transformations, mutational or epigenetic, occurrence as an accidental byproduct oncogenesis. As such, we present hypothesis that form...
Understanding the relationship between intracellular motion and macromolecular structure remains a challenge in biology. Macromolecular structures are assembled from numerous molecules, some of which cannot be labeled. Most techniques to study require potentially cytotoxic dyes or transfection, can alter cellular behavior susceptible photobleaching. Here we present multimodal label-free imaging platform for measuring dynamics living cells with sensitivity as small 20 nm millisecond temporal...
The progression of Alzheimer's disease (AD) is a continuous process in cognitive and biomarker changes, with only fraction mild impairment (MCI) patients eventually advancing to AD. There no definite signature determine this eventual progress. discovery development prognostic signatures for AD essential address the challenges drug development. deep learning (DL) technique recent breakthrough data science that enables researchers discover previously unknown features comprised by complicated...
Abstract Prior to the development of a localized cancerous tumor, diffuse molecular, and structural alterations occur throughout an organ due genetic, environmental, lifestyle factors. This process is known as field carcinogenesis. In this study, we used partial wave spectroscopic ( PWS ) microscopy explore progression carcinogenesis by measuring samples collected from 190 patients with range colonic history (no history, low‐risk high‐risk history) current colon health (healthy,...
Transformation in chromatin organization is one of the most universal markers carcinogenesis. Microscale alterations have been a staple histopathological diagnosis neoplasia, and nanoscale emerged as promising marker for cancer prognostication detection predysplastic changes. While numerous methods developed to detect these alterations, sample preparation remain largely validated via conventional microscopy not examined with sensitive imaging techniques. For techniques become standard care...
Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity biological samples.
Abstract In eukaryotic cells, chromatin structure is linked to transcription processes through the regulation of genome organization. Extending across multiple length-scales - from nucleosome higher-order three-dimensional structures a dynamic system which evolves throughout lifetime cell. However, no individual technique can fully elucidate behavior organization and its relation molecular function at all length- timescales both single-cell cell population level. Herein, we present...
Abstract The organization of chromatin is a regulator molecular processes including transcription, replication, and DNA repair. structures within that regulate these span from the nucleosomal (10nm) to chromosomal (>200nm) levels, with little known about dynamics structure between scales due lack quantitative imaging technique in live cells. Previous work using Partial Wave Spectroscopic (PWS) microscopy, sensitivity macromolecular 20-200nm, has shown transformation at length fundamental...
We demonstrate a multimodal imaging system that combines partial-wave spectroscopic microscopy and super-resolution to study chromatin topology. This instrument allows nanoscale visualization of labeled unlabeled with molecular structural contrast.
Chromatin is one of the most critical structures within cell because it houses genetic information. The higher-order organization chromatin structure crucial to gene regulation, and its dysregulation a hallmark many cellular pathological processes. well understood at nucleosomal level (<20nm) chromosomal (>200nm). However, due lack proper imaging quantitative modalities study this organization, particularly in live cells, little known about between these length scales. Currently, only...
Background Heart failure with preserved ejection fraction accounts for approximately half of all heart cases and is associated similar morbidity mortality. Although abnormalities diastolic function are felt to play an important role, no specific treatments have been identified this common condition, primarily because poor understanding its pathophysiology. Despite development several murine models disease, accurate non-invasive assessment has challenging. Echocardiographic measurements...
Abstract We present a multimodal label-free interferometric imaging platform for measuring intracellular nanoscale structure and macromolecular dynamics in living cells with sensitivity to macromolecules as small 20nm millisecond temporal resolution. validate this system by pairing experimental measurements of nanosphere phantoms novel theory. Applying vitro , we explore changes higher-order chromatin that occur due cellular fixation, stem cell differentiation, ultraviolet (UV) light...
One of the major challenges in population screening is identifying patients at risk cancer. For instance, colorectal cancer, while entire age 50 should be subject to screening, lifetime CRC only ~5%. Indeed, average colonoscopy can detect significant polyps <10% time. Screening for low prevalence lesions leads increase costs, unnecessary morbidity and most particularly false positives. Thus, stratification identify likely benefit from tests such as critical.
Abstract Proper regulation of higher-order chromatin structure is essential for normal gene and cellular function. We have previously found that the nanoscale significantly altered in early field carcinogenesis using novel spectroscopic methods parallel with biological assays (Backman Roy, J Cancer, 2013, 3:251-261; Subramanian et al, Cancer Res, 2009, 13:5357-63; Stypula-Cyrus PLoS One, 5:e64600). This was done fixed human animal model samples, suggesting genetic/epigenetic alterations can...
Abstract Background Synaptic vesicle glycoprotein 2A (SV2A) is a transmembrane synaptic protein that regulates exocytosis and neurotransmitter release. Positron emission tomography (PET) studies reveal the levels of SV2A are decreased in brains patients with Alzheimer’s disease (AD) other brain disorders. Whether changes PET signal caused by loss synapses or unclear an impediment to developing novel treatments for neurodegenerative diseases. The goal this study determine biological...
We demonstrate a multimodal imaging methodology to probe the nanoscale environment of cells. The system combines partial-wave spectroscopic (PWS) microscopy and photon localization (SPLM). PWS quantifies nanoarchitecture cells with sensitivity structures between 20 200 nm. SPLM is newly developed super-resolution technique based upon principles single-molecule spectroscopy. In addition allowing imaging, provides inherent molecular-specific information targeted visualized when dyes are used....