A5082756080- Genomics and Chromatin Dynamics
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Biology and Fertility
- Reproductive Health and Technologies
- Maternal and fetal healthcare
- RNA Research and Splicing
- Uterine Myomas and Treatments
- Ovarian function and disorders
- Prenatal Screening and Diagnostics
- Gene expression and cancer classification
- Endometriosis Research and Treatment
- Maternal and Perinatal Health Interventions
- Cancer Genomics and Diagnostics
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Plant Molecular Biology Research
- Advanced biosensing and bioanalysis techniques
- Systemic Lupus Erythematosus Research
- Chromosomal and Genetic Variations
- Single-cell and spatial transcriptomics
- Gynecological conditions and treatments
- Genomic variations and chromosomal abnormalities
- Advanced Biosensing Techniques and Applications
- Polymer composites and self-healing
- Renal Diseases and Glomerulopathies
Northwestern University
2020-2025
McCormick (United States)
2021
Massachusetts General Hospital
2019
Harvard University
2019
Texas A&M University
2018
University of the West Indies System
1996-2017
University of the West Indies
1992-2012
Genetics and IVF Institute
2010
Weatherford College
2008
Laguna Research
2007
Preimplantation genetic screening (PGS) has been used in an attempt to determine embryonic aneuploidy. Techniques that use new molecular methods the karyotype of embryo are expanding scope PGS.We introduce a method for PGS, termed 'parental support', which leverages microarray measurements from parental DNA 'clean' single-cell on cells and explicitly computes confidence each copy number call. The distinguishes mitotic meiotic errors determines source aneuploidy.Validation with 459 single...
nano-ChIA combines multiple nanoimaging modalities to uncover 4D chromatin organization and its relationship molecular function.
Chromatin organization over multiple length scales plays a critical role in the regulation of transcription. Deciphering interplay these processes requires high-resolution, three-dimensional, quantitative imaging chromatin structure vitro. Herein, we introduce ChromSTEM, method that utilizes high-angle annular dark-field and tomography scanning transmission electron microscopy combined with DNA-specific staining for microscopy. We utilized ChromSTEM an in-depth quantification 3D conformation...
To assess the comparative efficacy of perivascular vasopressin and tourniquet in minimizing bleeding its sequelae at myomectomy. Between March 1994 February 1995, 52 women with symptomatic uterine leiomyomas scheduled for myomectomy were entered into a randomized trial comparing (26 patients) hemostasis. Myomectomy was performed after either injection 20 U diluted to mL normal saline or use Foley catheter around both vessels. The each method measured by differences pre- postoperative...
To assess the efficacy of intramyometrial vasopressin for minimising bleeding and its sequelae at myomectomy.A randomised placebo controlled trial.University Hospital West Indies, Kingston, Jamaica.Twenty women with symptomatic uterine fibroids scheduled myomectomy who satisfied entry criteria: 10 to group control group.Myomectomy was performed after injection either 20 units diluted ml in normal saline or (20 saline).The measured by comparing pre- post-operative haemoglobin levels...
ABSTRACT Objective To determine if misoprostol (exogenous prostaglandin E 1 PGE ) used vaginally was of value in improving the Bishop score, leading to an early safe vaginal delivery women whom cervix is unripe and indicated. Design A double‐blind clinical trial. Setting Antenatal labour wards University Hospital West Indies, Jamaica. Subjects Forty‐five third trimester with various obstetric indications for induction no contra‐indications prostaglandins. Interventions The were randomly...
SignificanceSpectroscopic single-molecule localization microscopy (sSMLM) takes advantage of nanoscopy and spectroscopy, enabling sub-10 nm resolution as well simultaneous multicolor imaging multi-labeled samples. Reconstruction raw sSMLM data using deep learning is a promising approach for visualizing the subcellular structures at nanoscale.AimDevelop novel computational leveraging to reconstruct both label-free fluorescence-labeled data.ApproachWe developed two-network-model based...
The CPMC model demonstrates the regulatory role of chromatin’s physical structure on transcription and phenotypic plasticity.
In single cells, variably sized nanoscale chromatin structures are observed, but it is unknown whether these form a cohesive framework that regulates RNA transcription. Here, we demonstrate the human genome an emergent, self-assembling, reinforcement learning system. Conformationally defined heterogeneous, nanoscopic packing domains by interplay of transcription, nucleosome remodeling, and loop extrusion. We show not topologically associated domains. Instead, exist across structure-function...
Chromatin organization regulates transcription to influence cellular plasticity and cell fate. We explored whether chromatin nanoscale packing domains are involved in stemness response chemotherapy. Using an optical spectroscopic nanosensing technology we show that ovarian cancer-derived cancer stem cells (CSCs) display upregulation of compared non-CSCs. Cleavage under targets tagmentation (CUT&Tag) sequencing with antibodies for repressive H3K27me3 active H3K4me3 H3K27ac marks mapped...
Abstract Super-resolution microscopy has revolutionized our ability to visualize structures below the diffraction limit of conventional optical and is particularly useful for investigating complex biological targets like chromatin. Chromatin exhibits a hierarchical organization with structural compartments domains at different length scales, from nanometers micrometers. Single molecule localization (SMLM) methods, such as STORM, are essential studying chromatin supra-nucleosome level due...
Objective To determine whether hyoscine butylbromide shortens the first stage of labour, without an increase in maternal or neonatal complications. Design Randomised, double‐blinded, controlled trial. Setting The Antenatal clinics and Labour Delivery ward University Hospital West Indies, Kingston, Jamaica. Population Women spontaneous labour at term. Methods Either drug placebo was given intravenously once women entered active labour. Main outcome measures duration Secondary outcomes...
As imaging techniques rapidly evolve to probe nanoscale genome organization at higher resolution, it is critical consider how the reagents and procedures involved in sample preparation affect chromatin relevant length scales. Here, we investigate effects of fluorescent labeling DNA sequences within using gold standard technique three-dimensional fluorescence situ hybridization (3D FISH). The chemical 3D FISH protocol, specifically formamide, cause significant alterations sub-200 nm (sub-Mbp)...
Super-resolution microscopy has revolutionized our ability to visualize structures below the diffraction limit of conventional optical and is particularly useful for investigating complex biological targets like chromatin. Chromatin exhibits a hierarchical organization with structural compartments domains at different length scales, from nanometers micrometers. Single molecule localization (SMLM) methods, such as STORM, are essential studying chromatin supra-nucleosome level due their target...
This prospective study was designed to evaluate the operative morbidity and reproductive outcome in patients who had secondary myomectomy for recurrent symptomatic uterine fibroids.A total of 58 women were subjected a via abdominal route. The such as blood loss, presence adhesions febrile index estimated pregnancy over 2-4 year period follow-up.The mean age standard deviation (+/- SD) 35 2.4) years. Nineteen (33%) postoperative temperature vertical line 100 degrees F loss ranged from...
The metabolic plasticity of cancer cells is considered a highly advantageous phenotype that crucial for disease progression and acquisition treatment resistance. A better understanding metabolism its adaptability after treatments vital to develop more effective therapies. To screen novel therapies combination regimens, three-dimensional (3D) culture models cancers are attractive platforms as they recapitulate key features cancer. By applying non-perturbative intensity-based redox imaging...
ABSTRACT Current vascular aneurysm treatments often require either highly invasive strategy to surgically occlude an or endovascular occlusion via metal coils. While coils are safer, they have limited efficacy. Endovascular that integrated with shape memory polymer (SMP) foams the potential improve and reduce coil risks; however, mechanical performance homogeneity of SMP can hinder their effective use. To address this issue, synthesized using monomer diethanolamine (DEA) in place...
Blood samples from 50~women who had recurrent spontaneous abortions and 135 healthy multiparous women were investigated for anticardiolipin (aCL) antibodies anti-β2 Glycoprotein 1 (anti-β2 GP1) dependent aCL by enzyme-linked immuno
To determine the prevalence of genital Chlamydia trachomatis infection and risk factors in women attending family planning, gynaecology, sexually transmitted disease (STD) clinics Jamaica.Endocervical specimens from 645 including 238 170 237 STD clinic attendees were examined for C using a direct fluorescence assay (DFA) culture. Investigations carried out presence other pathogens demographic, behavioural, historical, clinical data recorded each participant.The was 35%, 47%, 55% clients,...
Blood samples from 50~women who had recurrent spontaneous abortions and 135 healthy multiparous women were investigated for anticardiolipin (aCL) antibodies anti-β₂ Glycoprotein 1 (anti-β₂ GP1) dependent aCL by enzyme-linked immunosorbent assays (ELISA), lupus anticoagulant activity was measured activated partial thromboplastin time, antinuclear antibodies, rheumatoid factors thyroid using standard techniques. Serological tests syphilis performed on all sera function evaluated. There no...
The transcriptional plasticity of cancer cells promotes intercellular heterogeneity in response to anticancer drugs and facilitates the generation subpopulation surviving cells. Characterizing single-cell after drug treatments can provide mechanistic insights into efficacy. Here, we used RNA-seq examine transcriptomic profiles treated with paclitaxel, celecoxib combination two drugs. By normalizing expression endogenous genes spike-in molecules, found that cellular mRNA abundance shows...