A5045593572- Mitochondrial Function and Pathology
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Endoplasmic Reticulum Stress and Disease
- Diet and metabolism studies
- Photosynthetic Processes and Mechanisms
- Genetic Neurodegenerative Diseases
- 3D Printing in Biomedical Research
- Cardiovascular Function and Risk Factors
- Genetics, Aging, and Longevity in Model Organisms
- Molecular Biology Techniques and Applications
- Cell Image Analysis Techniques
University of Iowa
2021-2024
Fraternal Order of Eagles
2021
Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria endoplasmic reticulum (ER) are organelles particular interest those investigating metabolic disorders. A straightforward method for quantifying characterizing aspects these would be a useful tool. In this protocol, we outline how accurately assess the morphology important structures using open source software ImageJ,...
This article shows how mitochondria in murine cardiac changes, importantly elucidating age-related changes. It also is the first to show that MICOS complex may play a role outer membrane mitochondrial structure.
Abstract During aging, muscle gradually undergoes sarcopenia, the loss of function associated with mass, strength, endurance, and oxidative capacity. However, 3D structural alterations mitochondria aging in skeletal cardiac tissues are not well described. Although mitochondrial is decreased capacity, genes responsible for morphological changes during poorly characterized. We measured morphology aged murine gastrocnemius, soleus, using serial block‐face scanning electron microscopy...
High-resolution 3D images of organelles are paramount importance in cellular biology. Although light microscopy and transmission electron (TEM) have provided the standard for imaging structures, they cannot provide images. However, recent technological advances such as serial block-face scanning (SBF-SEM) focused ion beam (FIB-SEM) tools to create ultrastructural analysis organelles. Here, we describe a standardized protocol using visualization software, Amira, quantify organelle...
Abstract Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content abundance. The physiological function bioenergetic capacity of mitochondria connected the structure, folding, organization inner‐membrane cristae. During aging process, mitochondrial dysfunction is observed, regulatory balance dynamics often disrupted, leading increased fragmentation in cells. Therefore, it hypothesized that significant...
2 ABSTRACT Transmission electron microscopy (TEM) is a scientific research standard for producing nanometer-resolution ultrastructural images of subcellular components within cells and tissues. Mitochondria, endoplasmic reticulum (ER), lysosomes, autophagosomes are organelles particular interest to those investigating metabolic disorders. However, there no clear consensus amongst regarding the best methods quantifying features in TEM images. In this protocol, we propose standardized approach...
Abstract The physical characteristics of brown adipose tissue (BAT) are defined by the presence multilocular lipid droplets (LDs) within adipocytes and a high abundance iron‐containing mitochondria, which give it its characteristic color. Normal mitochondrial function is, in part, regulated organelle‐to‐organelle contacts. For example, contact sites that mediate mitochondria–LD interactions thought to have various physiological roles, such as synthesis metabolism lipids. Aging is associated...
Abstract The sorting and assembly machinery (SAM) Complex is responsible for assembling β‐barrel proteins in the mitochondrial membrane. Comprising three subunits, Sam35, Sam37, Sam50, SAM complex connects inner outer membranes by interacting with contact site cristae organizing system complex. particular, stabilizes intermembrane space bridging (MIB) complex, which crucial protein transport, respiratory chain assembly, regulation of integrity. While role Sam50 structure metabolism skeletal...
ABSTRACT With sparse treatment options, cardiac disease remains a significant cause of death among humans. As person ages, mitochondria break down and the heart becomes less efficient. Heart failure is linked to many mitochondria-associated processes, including endoplasmic reticulum stress, mitochondrial bioenergetics, insulin signaling, autophagy, oxidative stress. The roles key complexes that dictate ultrastructure, such as contact site cristae organizing system (MICOS), in aging muscle...
Mitochondria are required for energy production and even give brown adipose tissue (BAT) its characteristic color due to their high iron content abundance. The physiological function bioenergetic capacity of mitochondria connected the structure, folding, organization inner-membrane cristae. During aging process, mitochondrial dysfunction is observed, regulatory balance dynamics often disrupted, leading increased fragmentation in cells. Therefore, we hypothesized that significant...
The physical characteristics of brown adipose tissue (BAT) are defined by the presence multilocular lipid droplets (LD) within adipocytes and a high abundance iron-containing mitochondria, which give it its characteristic color. Normal mitochondrial function is, in part, regulated organelle-to-organelle contacts. Particularly, contact sites that mediate mitochondria-LD interactions thought to have various physiological roles, such as synthesis metabolism lipids. Aging is associated with...
ABSTRACT Background During aging, muscle gradually undergoes loss of function including sarcopenia, losing mass, strength, endurance, and oxidative capacity. While mitochondrial aging is associated with decreased capacity, the genes morphological changes in mitochondria during still require further elucidation. Furthermore, it not completely understood how 3D structures are altered skeletal cardiac tissues. Methods We measured morphology complexity murine gastrocnemius, soleus, tissues using...
Aging The image representing article number 2300186 by Amber Crabtree, Zer Vue, Antentor Hinton Jr., and co-workers depicts 3D reconstruction of folds the inner mitochondrial membrane, known as cristae. team elucidate how cristae spatial organization morphology are altered across aging process in brown adipose tissue.
Abstract The Sorting and Assembly Machinery (SAM) Complex is responsible for assembling β-barrel proteins in the mitochondrial membrane. Comprising three subunits, Sam35, Sam37, Sam50, SAM complex connects inner outer membranes by interacting with contact site cristae organizing system (MICOS) complex. particular, stabilizes intermembrane space bridging (MIB) complex, which crucial protein transport, respiratory chain assembly, regulation of integrity. While role Sam50 structure metabolism...
Cover legend: The cover image is based on the Research Article 3D reconstruction of murine mitochondria reveals changes in structure during aging linked to MICOS complex by Zer Vue et al., https://doi.org/10.1111/acel.14009
ABSTRACT Analysis of 3D structures is paramount importance in cellular biology. Although light microscopy and transmission electron (TEM) have remained staples for imaging structures, they lack the ability to image 3D. However, recent technological advances, such as serial block-face scanning (SBF-SEM) focused ion beam (FIB-SEM), allowed researchers observe ultrastructure Here, we propose a standardized protocol using visualization software Amira quantify organelle morphologies 3D; this...