The green eukaryote alga Chlamydomonas reinhardtii is a unicellular model to study control of metabolism in photosynthetic organism. We here present method improvements for metabolite profiling based on GC-TOF mass spectrometry focusing three parameters: quenching and cell disruption, extract solvent composition annotation. These facilitate using smaller numbers hence, culture volumes which enable faster more precise sampling techniques that eventually lead higher number samples can be...

Alternative materials obtained from natural resources have recently garnered considerable attention as an innovative solution to bring unprecedented advances in various energy storage systems. Here, we present a new class of heterolayered nanomat-based hierarchical/asymmetric porous membrane with synergistically coupled chemical activity nanocellulose-mediated green material strategy develop smart battery separator membranes far beyond their current state-of-the-art counterparts. This...

Thermoplastic blends are applied for three-dimensional (3D) printing to obtain improved functionality. While thermal, chemical, and mechanical properties of 3D-printed typically examined, biodegradability the plastics has rarely been focus research. In this study, we evaluated biodegradation behavior prototypes fabricated from various blends, including biodegradable polylactic acid (PLA), poly(3-hydroxybutyrate) (PHB), non-biodegradable high-density polyethylene (HDPE), polypropylene (PP)....

Triple-negative breast cancer (TNBC) cells do not contain various receptors for targeted treatment, a reason behind the poor prognosis of this disease. In study, biocompatible theranostic erythrocyte-derived nanoparticles (EDNs) were developed and evaluated effective early diagnosis treatment TNBC. The anti-cancer drug, doxorubicin (DOX), was encapsulated into EDNs diagnostic quantum dots (QDs) incorporated lipid bilayers tumor bio-imaging. Then, anti-epidermal growth factor receptor (EGFR)...

mRNA vaccines have emerged as a pivotal tool in combating COVID-19, offering an advanced approach to immunization. A key challenge with these is their need for extremely-low-temperature storage, which affects stability and shelf life. Our research addresses this issue by enhancing the of through novel cationic lipid, O,O′-dimyristyl-N-lysyl aspartate (DMKD). DMKD effectively binds mRNA, improving vaccine stability. We also integrated phosphatidylserine (PS) into formulation boost immune...

Lycii Radicis Cortex (LRC) is a traditional medicine in East Asia with various beneficial effects, including antioxidant, anti-inflammatory, anti-tumor, anti-diabetic, and anti-depressant properties. However, its potential effects on skeletal muscle atrophy have not been studied. In this study, the protective of LRC extract (LRCE) dexamethasone (DEX)-induced were investigated C2C12 myotubes mice. We evaluated effect LRCE improving using variety methods, immunofluorescence staining,...

Skeletal muscle atrophy occurs when protein degradation exceeds synthesis and is associated with increased circulating glucocorticoid levels. Salvia plebeia R.Br. (SPR) has been used as herbal remedy for a variety of inflammatory diseases various biological actions such antioxidant anti-inflammatory activities. However, there are no reports on the effects SPR its bioactive components atrophy. Herein, we investigated anti-atrophic effect rosmarinic acid (RosA), major compound SPR,...

The electrical properties of thermally grown and atomic-layer-deposition (ALD) oxides, followed by nitridation treatment, on 4H-SiC substrate were compared. treatment was performed with post oxidation annealing in NO atmosphere (NO POA). best characteristics the ALD oxides observed at 120 180 min POA, respectively. POA treated oxide showed extremely low interface trap density (Dit), less than 1011 eV−1 cm−1. A metal-oxide-semiconductor field-effect-transistor high field effect mobility,...

Saururus chinensis has been used in folk medicine Korea for the treatment of edema, jaundice, gonorrhea, and several inflammatory diseases. Saururi extracts (SCE) have demonstrated anti-inflammatory anti-oxidant activities, as well anti-asthmatic, antihypertensive, anti-angiogenic, therapeutic activities atopic dermatitis. However, inhibitory activity SCE on melanogenesis signaling pathway is not completely understood. This study examined effects activated by α-melanocyte-stimulating hormone...

Application of growth factor mixtures has been used for wound healing and anti-wrinkles agents. The aim this study was to evaluate the effect recombinant (RGFM) on expression cell cycle regulatory proteins, type I collagen, processes acute animal models. results showed that RGFM induced increased rates proliferation migration human skin fibroblasts (HSF). In addition, cyclin D1, E, cyclin-dependent kinase (Cdk)4, Cdk2 proteins markedly with a treatment in fibroblasts. Expression collagen...

Many different types of nanoparticles have been suggested for tumor-targeted theranosis. However, most systems were prepared through a series complicated processes and could not even overcome the blood–immune barriers. For accurate diagnosis effective treatment cancers, herein we lipid micellar structure capturing quantum dot (QD) cancer The QD/lipid micelles (QDMs) using simple self-assembly procedure then conjugated with anti-epidermal growth factor receptor (EGFR) antibodies tumor...

was grown by dry thermal oxidation (at 1175, 1300, or ) on n-type 4H-SiC substrates. The samples were prepared subsequently exposing the film to postoxidation annealing (POA) treatment using nitric oxide (NO) gas. interfaces characterized high frequency capacitance–voltage measurements, X-ray photoelectron spectroscopy (XPS), and ellipsometry. interface trap density of at much lower than others. At a higher temperature , electrical physical properties not improved compared those oxides . XPS...

Abstract Oxidative stress has been implicated in the pathogenesis of neuronal degenerative diseases. It is also widely known that oxidative induces mitogen-activated protein kinase (MAPK) signaling cascades. In this study, we used proteomic analysis to investigate role MAPK pathway stress-induced cell death. The results demonstrated several proteins, including eukaryotic translation elongation factor 2 (eEF2) and enolase I, showed a differential expression pattern during death process, was...

Understanding animals’ genetic potential for carcass traits is the key to improvements in any beef cattle. In this study, we investigated merits of using Hanwoo males raised a 30-month production system (PROD30). We achieved dataset comprising 6092 born between 2005 and 2017 measures four (carcass weight, CWT; eye muscle area, EMA; backfat thickness, BFT; marbling score, MS). Genetic parameters were estimated multiple-trait animal model through AIREMLF90 program. According model, h2 CWT,...

In this letter, we have reported electrical and physical properties of rapid thermal annealed (RTA) Al2O3 stacking dielectric on n-type 4H-SiC. The effects SiN-reaction barrier layer (RBL) between thermal-nitrided SiO2 SiC-based metal-oxide-semiconductor characteristics been investigated compared. A significant reduction in oxide-semiconductor interface-trap density (Dit), improvement breakdown field reliability has observed after the SiN-RBL introduced SiO2. High-resolution transmission...

The mechanisms of the thermal decomposition -triglyme, and -tetraglyme, where is bis(dipivaloylmethanato)strontium, were examined using thermogravimetric analysis, mass spectrometry, Fourier transform infrared spectroscopy (FTIR). chemical bonds in Sr compounds are sequentially decomposed as heated. That is, glyme adducts at temperatures below 200°C, dissociated higher temperatures, whereas remain stable up to 400°C. Glyme weaken bond between central atom dpm ligand, and, a result, -glymes...

Avoiding phagocytic cells and reducing off-target toxicity are the primary hurdles in clinical application of nanoparticles containing therapeutics. For overcoming these errors, this study, expressing CD47 proteins inhibiting attack immune were prepared then evaluated as an anti-cancer drug delivery vehicle.The CD47+ cell-derived (CDNs) from plasma membranes human embryonic kidney transfected with a plasmid encoding CD47. And doxorubicin (DOX) was loaded into CDNs, anti-EGF receptor (EGFR)...

This study examined whether treatment with <i>Phyllostachyos Caulis in Taeniam</i> aqueous extract improves longitudinal bone growth adolescent male rats. Three-week-old Sprague-Dawley rats were divided into three groups: a control group, group (200 mg/kg, p. o.), and recombinant human hormone (20 µg/kg, s. c.). The total tibial length the height of each plate zone evaluated by radiography histomorphometry. number proliferative cells 5-bromo-2′-deoxyuridine-positive counted after...