Abstract KIF1A is a brain-specific anterograde motor protein that transports cargoes towards the plus-ends of microtubules. Many variants gene have been associated with neurodegenerative diseases and developmental delay. Homozygous mutations identified in recessive subtype hereditary spastic paraplegia (HSP), SPG30. In addition, found pure HSP autosomal dominant inheritance. Here we report first case familial complicated mutation transmitted A heterozygous p.T258M was Korean family through...

ABSTRACT A pressure collar, assembled around 25cm sections of 4‐year‐old willow twigs, was used to examine cavitation events under field conditions. When the air inside collar raised between 1–8 and 2–8MPa, ultrasound acoustic emission signals were triggered which indicated breaking water columns in xylem. The hydraulic conductivity twig portion chamber decreased markedly. As a result, potentials conductances leaves at end decreased. Similar changes induced comparable pressures detached...

We developed a hybrid microfluidic device that utilized acoustic waves to drive functionalized microparticles inside continuous flow microchannel and separate particle-conjugated target proteins from complex fluid. The acoustofluidic is composed of an interdigitated transducer produces high-frequency surface (SAW) polydimethylsiloxane (PDMS) channel. SAW interacted with the sample fluid deflected particles their original streamlines achieve separation. Streptavidin-functionalized polystyrene...

Cloned animals developed from somatic cell nuclear transfer (SCNT) embryos are useful resources for agricultural and medical applications. However, the birth rate in cloned is very low, that have survived show various developmental defects. In this report, we present morphology differentially regulated proteins extraembryonic tissue SCNT to understand molecular nature of tissue. We examined 26-day-old porcine at which sonogram can first detect pregnancy. The was abnormally small compared...

Solvent-based synthetic methods of fullerene nano/microstructures are known to enhance and utilize unique optical electrical properties structures. Here, we report the systematic synthesis characterization various-shaped microcrystals using alcohols as antisolvents in drowning-out crystallization. The formed one-, two-, three-dimensional structures depending on alcohol type, size shape also varied by C60 concentration volume ratio solvents. X-ray diffraction patterns demonstrate that...

We propose an acoustofluidic method for the triseparation of proteins conjugated with aptamer-coated microparticles inside a microchannel. Traveling surface acoustic waves (TSAWs) produced from slanted-finger interdigital transducer (SFIT) are used to separate protein-loaded different sizes via TSAW-driven radiation force (ARF). The device consists SFIT deposited onto piezoelectric lithium niobate substrate and polydimethylsiloxane (PDMS) microfluidic channel on top substrate. TSAWs...

Cdc25 phosphatases have been considered as attractive drug targets for anticancer therapy because of the correlation their overexpression with a wide variety cancers. We able to identify five novel phosphatase inhibitors micromolar activity by means computer-aided design protocol involving homology modeling Cdc25A and virtual screening automated AutoDock program implementing effects ligand solvation in scoring function. Because newly discovered are structurally diverse reveal significant...

Several natural substances including protein produce sweet taste. Brazzein, derived from the plant Pentadipladra brazzeana, is one of proteins that bind to taste receptor with stronger sweetness than sugar. Mutations this affect its flavour, yielding higher in D29K and lower R43A. To elucidate mechanism receptor, we determined structures two variants, R43A, a resolution 1.5 Å 1.3 Å, respectively. Structures brazzein exhibit α-helix three β-sheets connected by four disulfide bonds...

Mitogen-activated protein kinases (MAPKs) mediate a variety of cell signals regulating growth, differentiation, transcription, and metabolism. MAPKs are fully activated by dual phosphorylation on tyrosine threonine in their activation loops. This process is thought to be counteracted family proteins termed dual-specificity phosphatases (DSPs). Inadequate production or action DSPs has been associated with diverse human disorders, including cancer, diabetes, autoimmune diseases.1-4 subclass...

Oxidative stress is one of the major causes neuronal cell death in disorders such as perinatal hypoxia and ischemia. Protein phosphorylation most significant PTM proteins plays an important role stress-induced signal transduction. Thus, analysis alternative protein states which occur during oxidative could provide valuable information regarding death. In this study, a reference phosphoproteome map mouse hippocampal line HT22 was constructed based on 125 spots that were identified by...

Cyclophilins belong to a family of proteins that bind the immunosuppressive drug cyclosporin A (CsA). Several members this protein catalyze cis–trans isomerization peptide bonds preceding prolyl residues. The present study describes biochemical and structural characteristics cytosolic cyclophilin (TaCypA-1) cloned from wheat (Triticum aestivum L.). Purified TaCypA-1 expressed in Escherichia coli showed peptidyl-prolyl isomerase activity, which was inhibited by CsA with an inhibition constant...

Abstract The testis‐ and skeletal‐muscle‐specific dual‐specificity phosphatase (TMDP) is a member of the (DSP) subgroup protein tyrosine phosphatases. TMDP has similar activities toward both threonine phosphorylated substrates, supposed to be involved in spermatogenesis. Here, we report crystal structure human at resolution 2.4 Å. In spite high sequence similarity with other DSPs, shows distinct structural motifs surface properties. TMDP, α1–β1 loop, substrate recognition motif located...

Vegetable and fruit wastes mixed with sawdust were composted in a laboratory scale reactor by controlling the waste feeding rate at 21 kg m(-3) day(-1) aeration 10.6 l min(-1). The effects of initial moisture content on organic matter degradation process performance fed batch composting investigated. absolute amount removal, removal percentage, dry mass obtained substantially different among contents. rapid rise lowest observed achieved 50% condition. yielding largest both curing stage was...

Dual specificity protein tyrosine phosphatases (DSPs) play an important role in controlling various cellular processes, including cell growth, differentiation, transcription, and metabolism by catalyzing the hydrolysis of phosphorylated substrates.1 To meet demand for distinct functions implicated diverse signaling, human genome is estimated to encode 61 DSPs among 107 (PTPs).2 According their structural functional characteristics, can be further divided into seven groups.2 MKPs atypical...

Abstract Western blot is a principal technique for the detection of specific proteins in various biology disciplines. The antibody incubation an imperative step western blot. Antibody by mild shaking on rocker generally used to facilitate mixing antibodies. However, inefficient process remove depletion layer blotting membrane and requires hours time achieve binding target proteins. We propose alternative method cyclic draining replenishing (CDR) solution using rotational chamber. study...

Abstract Oxidative stress has been implicated in the pathogenesis of neuronal degenerative diseases. It is also widely known that oxidative induces mitogen-activated protein kinase (MAPK) signaling cascades. In this study, we used proteomic analysis to investigate role MAPK pathway stress-induced cell death. The results demonstrated several proteins, including eukaryotic translation elongation factor 2 (eEF2) and enolase I, showed a differential expression pattern during death process, was...

Protein tyrosine phosphatases (PTPs) play important roles in cellular signal transduction mediating cell growth, differentiation, transcription, and metabolism.1 PTPs have been regarded as a drug target for human disorders such cancer, diabetes, autoimmune disease according to their distinct mode of actions.1, 2 The PTP family, which consists 107 members, can be divided into three classes Cys-based class Asp-based substrate specificity structural characteristics.2 include 38 classical 61...

Abstract Tardigrades are microscopic animals widely known for their ability to survive in extreme conditions. They the focus of current research fields taxonomy, biogeography, genomics, proteomics, development, space biology, evolution and ecology. Tardigrades, such as Hypsibius exemplaris, being advocated a next-generation model organism genomic developmental studies. The raw culture H. exemplaris usually contains tardigrades themselves, eggs, faeces algal food. Experimentation with often...

Eosinophils act as effectors in the inflammatory reactions of allergic diseases including atopic dermatitis. Atopic dermatitis patients and others with disorders suffer from eosinophilia, an accumulation eosinophils due to increased survival or decreased apoptosis eosinophils. In this study, a differential phosphoproteome analysis AML14.3D10 eosinophil cell line after treatment IL-5 dexamethasone was conducted effort identify phosphoproteins involved proliferation Proteins were separated by...

mCardinal2 is a red fluorescent protein developed through the designs of mKate, mNeptune and mCardinal. Fluorescence spectrums its five mutants (T143C, T143G, C158A, C158D M160E) were measured with their quantum yields. C158A increased brightness slight changes in fluorescence while T143C, T143G M160E decreased blue shift spectrums, which resulted green, cyan green proteins respectively. Crystal structures all six variants analyzed compared together those LSS-mKate1, LSS-mKate2 Around Cα-Cβ...